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The map label for this gene is napB [H]

Identifier: 117920346

GI number: 117920346

Start: 2257970

End: 2258455

Strand: Direct

Name: napB [H]

Synonym: Shewana3_1901

Alternate gene names: 117920346

Gene position: 2257970-2258455 (Clockwise)

Preceding gene: 117920345

Following gene: 117920347

Centisome position: 45.41

GC content: 49.79

Gene sequence:

>486_bases
ATGAAAACATTCAAATTAGTCTCAGCATTCAGTGTGCTTGCCTTCTCCACCCTGATGATGACCAGCGTGACTGCGGCGAG
CATTCCTGAGGACAGAATTGATACTTTGCGTCAGGCGCCAATCAACGTTGAGCCAACCCCGCCCGCGATGCAGGCGGTGC
TGAACAAAGATATCAAAGAGATGCGTAACTACCCGATGCAGCCACCAGTGATCCCGCACAAGATCGACGGTTATCAGCTC
GATCTTAAGGTCAACAAGTGTATGTCATGTCATGCCAGACACCGCACGGGGGACTCCCAAGCGCCTATGGTCAGCGTGAC
TCACTATATGGATAGGGATAACAATTTCTTAGCTGAGCTTTCGCCAAGACGTTACTTCTGCACTCAGTGCCATGTGCCGC
AACTGGATGCCAAATTACTGGTCGAGAACGACTTTGTGGATATGGATCATCTGATTAAAGCGAACACTGAGCAGAAAACA
CACTAG

Upstream 100 bases:

>100_bases
CTCATTAACAAAGTCACCTTGGATGCGACAGATCCTCTGTCAAAACAAACCGACTTTAAGAAATGCGCCGTGAAAATCGT
CAAGGTATAAGGAGTACAGG

Downstream 100 bases:

>100_bases
GAGTCAGCATGTTAGATAAACTCAAAAAGATCTGGCAAGTTCTCAATCGTCCGAGTGTGCACTACAGTTTAGGGTTTTTA
ACCTTAGGTGGCTTTGTGGC

Product: periplasmic nitrate reductase subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKTFKLVSAFSVLAFSTLMMTSVTAASIPEDRIDTLRQAPINVEPTPPAMQAVLNKDIKEMRNYPMQPPVIPHKIDGYQL
DLKVNKCMSCHARHRTGDSQAPMVSVTHYMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMDHLIKANTEQKT
H

Sequences:

>Translated_161_residues
MKTFKLVSAFSVLAFSTLMMTSVTAASIPEDRIDTLRQAPINVEPTPPAMQAVLNKDIKEMRNYPMQPPVIPHKIDGYQL
DLKVNKCMSCHARHRTGDSQAPMVSVTHYMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMDHLIKANTEQKT
H
>Mature_161_residues
MKTFKLVSAFSVLAFSTLMMTSVTAASIPEDRIDTLRQAPINVEPTPPAMQAVLNKDIKEMRNYPMQPPVIPHKIDGYQL
DLKVNKCMSCHARHRTGDSQAPMVSVTHYMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMDHLIKANTEQKT
H

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=83, Percent_Identity=49.3975903614458, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18344; Mature: 18344

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFKLVSAFSVLAFSTLMMTSVTAASIPEDRIDTLRQAPINVEPTPPAMQAVLNKDIKE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
MRNYPMQPPVIPHKIDGYQLDLKVNKCMSCHARHRTGDSQAPMVSVTHYMDRDNNFLAEL
HHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEC
SPRRYFCTQCHVPQLDAKLLVENDFVDMDHLIKANTEQKTH
CCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTFKLVSAFSVLAFSTLMMTSVTAASIPEDRIDTLRQAPINVEPTPPAMQAVLNKDIKE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
MRNYPMQPPVIPHKIDGYQLDLKVNKCMSCHARHRTGDSQAPMVSVTHYMDRDNNFLAEL
HHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEC
SPRRYFCTQCHVPQLDAKLLVENDFVDMDHLIKANTEQKTH
CCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]