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The map label for this gene is napA [H]

Identifier: 117920345

GI number: 117920345

Start: 2255470

End: 2257959

Strand: Direct

Name: napA [H]

Synonym: Shewana3_1900

Alternate gene names: 117920345

Gene position: 2255470-2257959 (Clockwise)

Preceding gene: 117920344

Following gene: 117920346

Centisome position: 45.36

GC content: 51.04

Gene sequence:

>2490_bases
ATGAATAGACGTGACTTTATCAAAGCCAATGCGGTGATTGCAGCAGCGGGCGCCGCAGGCTTAGCGCTGCCGGCATCGGC
GAGCAATTTAATCACCAGCTCAGAACAAACCAAATTAGAGTGGAACAAAGCCCCATGCCGCTTCTGTGGTACGGGCTGCT
CTGTGATTGTCGCGACCCGCGAGGGCAAAGTAGTTGCTACCCACGGCGATGCAAACAGTGAAGTGAACCGCGGCCTTAAC
TGTATTAAAGGTTATTTCCTGTCGAAAATTATGTACGGCAGCGATCGTTTGACCACGCCAATGCTGAGAATGACCGACGG
CAAATACGACAAGCATGGTGAGTTTACTCCCATCAGCTGGGACAGTGCCTTCGATATCATGGCGCAAAAATGGAAAGAAA
CCTTAAAAGCTAAAGGCCCGACCGCGGTTGGGATGTTTGGCTCGGGTCAGTGGACCGTTTGGGAAGGCTATGCCGCCGTT
AAATTGATGAAGGCCGGTTTTGGCTCTAACAACATCGACCCGAATGCGCGTCACTGTATGGCGTCTGCCGTTGTCGGCTT
TATGCGTACCTTCGGTATCGATGAGCCTATGGGCTGTTACGATGATATGGAAGCGGCCGATGCCTTTGTGCTTTGGGGCT
CCAACATGGCCGAAATGCACCCGATCTTATGGACTCGAGTGACTGACCGTCGTTTAAGCGCATCCCATGTGAAGGTGGCT
GTACTCTCGACGTTTGAGCATCGCTCCTTCGATCTCGCGGATTTACCGATAGTATTTACGCCACAAACCGACTTAGCCAT
CCTAAACTTTATCGCCAACTACATTATTCAAAACGATAAAGTGAACTGGGACTTTGTGAAGAAGCACGTTAACTTCCGCC
AAGGTACCACGGATATCGGCTATGGTTTACGTCCAACCCATCCGCTGGAGCAGAAAGCCAAAAACGTGAAAACGGCAGGG
GATTCGACTCCAATTGATTTCGACACCTTTAAGGCTTTTGTCGCCGAATATACGGTGGAGAAGGTCAGCAAGATGTCGGG
CGTACCTGAAGCTAAACTTATCGAGTTAGCTAAACTCTATGCCGATCCCGAGACTAAAGTCACGTCCTTCTGGACCATGG
GCTTCAACCAACATACCCGTGGTGTATGGTGTAACAACCTGATTTATAACATCCACTTACTGACAGGTAAGATTTCAACG
CCCGGTAACAGCCCGTTCTCGTTAACCGGTCAGCCTTCTGCCTGTGGTACGGCTCGCGAAGTGGGAACCTTCTCACACCG
TTTACCTGCCGACATGGTGGTAACAAACCCTGAGCATAGAAAACACGCCGAAGAAATTTGGAAGATCCCCAGTGGGATTA
TTCCGCCAAAACCCGGTTATCACGCCGTCGAGCAAAACCGCCGCTTAAAAGATGGCGACTTGAACTGCTATTGGGTGCAA
GTGAACAACAATATGCAGGCGGGGCCGAACATCAACGAGGAAGGCTTACCTGGCTATCGTAATCCTGCCAACTTTATTGT
GGTGTCCGATGCGTATCCCACTGTCACGACTCAGGCCGCGGACTTAATTCTGCCAACCGCCATGTGGGTTGAGAAAGAGG
GTGCCTACGGTAATGCCGAGCGTCGTACTCAATTCTGGCATCAAATGGTGCAGGCGCCGGGCGAATCTCACTCGGATCTG
TGGCAATTGATGGAGTTCTCTAAGCGCTTTACGACCGATGAAGTCTGGTCGAAGGAAGTCCTAGCGGCGAATCCACAATT
TAAGGGCAAAACCTTATTTGAAGTGCTGTACAAAAACGGCAATGTCGATAAGTTCCCGCTGGCCGATACCGATCCTAAGT
ATTTGAACAACGAAGCGAAACATTTTGGCTTCTATGTCCAAAAAGGCCTGTTTGAAGAATACGCCACCTTTGGCCGTGGC
CATGGTCACGATTTAGCACCTTTCGATGCCTACCACGAAGCCCACGGTATTCGCTGGCCGGTGGTCGATGGTAAAGAAAC
CAAATGGCGTTTCCGTGAGGGCAGCGATCCTTATGTCAAACCTGGCTCAGGCTTTGAGTTTTACGGTAAACCCGATGGCC
GCGCAGTGATTTATGCCTTGCCCTTCGAGCCGCCCGCAGAGTCGCCAGACACTGAATACGATATGTGGTTATCCACCGGA
CGGGTGCTTGAGCATTGGCATTCAGGTTCGATGACTCAACGCGTGCCAGAGCTTTACCGTGCCTTCCCGGATGCCGTGTG
TTTTATGCACCCAGAAGATGCGAAAAAACGCGGTTTACGCCGTGGGGATGAAGTGCGCGTGGTGTCTCGCCGCGGTGAAA
TCAAAACCCGTGTCGAAACCCGTGGTCGTAACAAACCACCAGTGGGACTCGTGTTCGTACCTTGGTTCGATGCCAGTCAG
CTCATTAACAAAGTCACCTTGGATGCGACAGATCCTCTGTCAAAACAAACCGACTTTAAGAAATGCGCCGTGAAAATCGT
CAAGGTATAA

Upstream 100 bases:

>100_bases
TGATGGACGGTATCGAACAGATTAATGCCATTCCTGGCGTCTTGTCCGCCACTATGGTTTACCACCAGAGTGAAGAGCTC
GAAGAGGGTGAAGAATGAAC

Downstream 100 bases:

>100_bases
GGAGTACAGGATGAAAACATTCAAATTAGTCTCAGCATTCAGTGTGCTTGCCTTCTCCACCCTGATGATGACCAGCGTGA
CTGCGGCGAGCATTCCTGAG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MNRRDFIKANAVIAAAGAAGLALPASASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATREGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFTPISWDSAFDIMAQKWKETLKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWTRVTDRRLSASHVKVA
VLSTFEHRSFDLADLPIVFTPQTDLAILNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFDTFKAFVAEYTVEKVSKMSGVPEAKLIELAKLYADPETKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEEIWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPANFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFSKRFTTDEVWSKEVLAANPQFKGKTLFEVLYKNGNVDKFPLADTDPKYLNNEAKHFGFYVQKGLFEEYATFGRG
HGHDLAPFDAYHEAHGIRWPVVDGKETKWRFREGSDPYVKPGSGFEFYGKPDGRAVIYALPFEPPAESPDTEYDMWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEVRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Sequences:

>Translated_829_residues
MNRRDFIKANAVIAAAGAAGLALPASASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATREGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFTPISWDSAFDIMAQKWKETLKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWTRVTDRRLSASHVKVA
VLSTFEHRSFDLADLPIVFTPQTDLAILNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFDTFKAFVAEYTVEKVSKMSGVPEAKLIELAKLYADPETKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEEIWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPANFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFSKRFTTDEVWSKEVLAANPQFKGKTLFEVLYKNGNVDKFPLADTDPKYLNNEAKHFGFYVQKGLFEEYATFGRG
HGHDLAPFDAYHEAHGIRWPVVDGKETKWRFREGSDPYVKPGSGFEFYGKPDGRAVIYALPFEPPAESPDTEYDMWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEVRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV
>Mature_829_residues
MNRRDFIKANAVIAAAGAAGLALPASASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATREGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFTPISWDSAFDIMAQKWKETLKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWTRVTDRRLSASHVKVA
VLSTFEHRSFDLADLPIVFTPQTDLAILNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTPIDFDTFKAFVAEYTVEKVSKMSGVPEAKLIELAKLYADPETKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKHAEEIWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPANFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFSKRFTTDEVWSKEVLAANPQFKGKTLFEVLYKNGNVDKFPLADTDPKYLNNEAKHFGFYVQKGLFEEYATFGRG
HGHDLAPFDAYHEAHGIRWPVVDGKETKWRFREGSDPYVKPGSGFEFYGKPDGRAVIYALPFEPPAESPDTEYDMWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEVRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=829, Percent_Identity=71.2907117008444, Blast_Score=1268, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=578, Percent_Identity=25.0865051903114, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI3868720, Length=666, Percent_Identity=22.5225225225225, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI3868719, Length=481, Percent_Identity=23.4927234927235, Blast_Score=100, Evalue=7e-22,
Organism=Escherichia coli, GI1787870, Length=884, Percent_Identity=23.4162895927602, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI87081797, Length=892, Percent_Identity=21.8609865470852, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92894; Mature: 92894

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPASASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
EGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFTPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETLKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWTRVTDRRLSASHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHCCCCEEEECCCCCCCCCEEEE
VLSTFEHRSFDLADLPIVFTPQTDLAILNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTPIDFDTFKAFVAEYTVEKVSKMSGVPEAKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPETKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKHAEEIWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPANFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFSKRFTTDEVWSKEVLAANPQFKGKTLFEVLYKNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCHHHHHHHHCC
NVDKFPLADTDPKYLNNEAKHFGFYVQKGLFEEYATFGRGHGHDLAPFDAYHEAHGIRWP
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKPGSGFEFYGKPDGRAVIYALPFEPPAESPDTEYDMWLSTG
CCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEVRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC
>Mature Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPASASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
EGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFTPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETLKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWTRVTDRRLSASHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHCCCCEEEECCCCCCCCCEEEE
VLSTFEHRSFDLADLPIVFTPQTDLAILNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTPIDFDTFKAFVAEYTVEKVSKMSGVPEAKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPETKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKHAEEIWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPANFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFSKRFTTDEVWSKEVLAANPQFKGKTLFEVLYKNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCHHHHHHHHCC
NVDKFPLADTDPKYLNNEAKHFGFYVQKGLFEEYATFGRGHGHDLAPFDAYHEAHGIRWP
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKPGSGFEFYGKPDGRAVIYALPFEPPAESPDTEYDMWLSTG
CCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEVRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA