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The map label for this gene is hisF [H]

Identifier: 117920293

GI number: 117920293

Start: 2190056

End: 2190829

Strand: Reverse

Name: hisF [H]

Synonym: Shewana3_1848

Alternate gene names: 117920293

Gene position: 2190829-2190056 (Counterclockwise)

Preceding gene: 117920294

Following gene: 117920292

Centisome position: 44.06

GC content: 53.36

Gene sequence:

>774_bases
ATGCTGGCCAAACGAATAGTCCCCTGTTTAGATGTGAAAGATGGCTGCGTGGTCAAGGGCGTACAGTTTCGCAACCATGA
AATTGTTGGCGACATAGTACCTCTCGCGGCGCGTTACGCCGCAGAGGGCGCCGACGAACTGGTGTTTTACGATATCACCG
CCAGTGCCCATGACCGAGTGGTCGATAAGTCTTGGGTGAGCCGAGTGGCGGAGCAAATCGATATCCCCTTCTGCGTTGCC
GGCGGCATTAAAACCATCGGCCAGGCGCGCGAGTTACTGGCCTTTGGCGCGGATAAGATTTCGGTCAACTCACCCGCTCT
TAGCGATCCGAGTTTAATCTCACGCCTGCAGGATGAATTCGGCCGTCAATGCATAGTGATCGGCATAGACTCTTTTTATG
ATGCGGCAAGCGACAGTTATAAGGTGAAGCAATTTACCGGCGATGAAGCCGCCACCAAAGAAACCGCTTGGTACACTCAG
GATTGGGTCGAAGAAGTACAAAAACGCGGCTGCGGCGAAATCGTGCTCAACGTGATGAACCAAGATGGCGTGCGCGGTGG
CTATGATATCAAACAGTTAAGCCTAGTACGCCAGCTGTGCGATGTGCCCTTAATCGCCTCGGGCGGCGCTGGCACTATGA
CACATTTTCGCGATGTGTTTATCGAGGCTAAGGTGGATGCAGCGCTAGCGGCCAGCGTGTTCCATAAAGCCATTATCAAT
ATCGGCGAGTTAAAACAGTATTTAGCGGCCGAAGGCATCGCCATCCGCCAGTAA

Upstream 100 bases:

>100_bases
CCCTTGAGGATGTTGCCGCCGTCAGAGACAGTGGCGCCGCGGGGATCATTATCGGTAAAGCTTTACTTATCAATCAGTTT
AATGTGGCGGAGGCAATCCA

Downstream 100 bases:

>100_bases
CTCAACATCAGTAGCGATTGATAGAAATAATAAGGTATACAGATATGACAACTGGAACCACTTCAAGCTGTTGCCAAGGC
CCTGCAAGCGCAGAACTGGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIVPCLDVKDGCVVKGVQFRNHEIVGDIVPLAARYAAEGADELVFYDITASAHDRVVDKSWVSRVAEQIDIPFCVA
GGIKTIGQARELLAFGADKISVNSPALSDPSLISRLQDEFGRQCIVIGIDSFYDAASDSYKVKQFTGDEAATKETAWYTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRQLCDVPLIASGGAGTMTHFRDVFIEAKVDAALAASVFHKAIIN
IGELKQYLAAEGIAIRQ

Sequences:

>Translated_257_residues
MLAKRIVPCLDVKDGCVVKGVQFRNHEIVGDIVPLAARYAAEGADELVFYDITASAHDRVVDKSWVSRVAEQIDIPFCVA
GGIKTIGQARELLAFGADKISVNSPALSDPSLISRLQDEFGRQCIVIGIDSFYDAASDSYKVKQFTGDEAATKETAWYTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRQLCDVPLIASGGAGTMTHFRDVFIEAKVDAALAASVFHKAIIN
IGELKQYLAAEGIAIRQ
>Mature_257_residues
MLAKRIVPCLDVKDGCVVKGVQFRNHEIVGDIVPLAARYAAEGADELVFYDITASAHDRVVDKSWVSRVAEQIDIPFCVA
GGIKTIGQARELLAFGADKISVNSPALSDPSLISRLQDEFGRQCIVIGIDSFYDAASDSYKVKQFTGDEAATKETAWYTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRQLCDVPLIASGGAGTMTHFRDVFIEAKVDAALAASVFHKAIIN
IGELKQYLAAEGIAIRQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=73.828125, Blast_Score=396, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=28.6624203821656, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27969; Mature: 27969

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDVKDGCVVKGVQFRNHEIVGDIVPLAARYAAEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTIGQARELLAFGADKISVNSPALSDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFYDAASDSYKVKQFTGDEAATKETAWYTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEECCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRQLCDVPLIASGGAGTMTHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKQYLAAEGIAIRQ
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIVPCLDVKDGCVVKGVQFRNHEIVGDIVPLAARYAAEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTIGQARELLAFGADKISVNSPALSDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFYDAASDSYKVKQFTGDEAATKETAWYTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEECCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRQLCDVPLIASGGAGTMTHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKQYLAAEGIAIRQ
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA