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The map label for this gene is holB [H]

Identifier: 117920214

GI number: 117920214

Start: 2080896

End: 2081852

Strand: Direct

Name: holB [H]

Synonym: Shewana3_1767

Alternate gene names: 117920214

Gene position: 2080896-2081852 (Clockwise)

Preceding gene: 117920213

Following gene: 117920215

Centisome position: 41.85

GC content: 46.92

Gene sequence:

>957_bases
ATGGTAGCGCAAAGCGGATTTGATGTATTAACCCTGCCTTGGCTCGATACGCCAAGACAGGCGTTTTTAACCCAGTTACA
AACCCAAAAAGTCCCCCACGCGCAACTCGTGGGTATCGATTCCGCCTATGGCGGTGAACTCTTGAGTGTGTTTATGGCGC
GTGCCGCCATGTGTTCACAACCCACTCCTGCGGGCGGATGCGGTTTTTGTAAGTCCTGCCAGCTATTTGATGCGGGTAAT
CACCCTGATTTTTATCAAATTGAGGCCGATGGTCATCAAATTAAAGTCGACCAAATTCGTGAGCTGTGTACTCGCCTGAG
TGCAACCGCCCAGCAGAGTGGTCGGCGTGTCGCCATCATTCACCACAGCGAGCGCTTAAACTCTGCTTCTGCCAATGCGT
TACTCAAGACGCTTGAAGAACCTGGCAAAGACACTTTACTGCTATTACATTCCGATACGCCTGCGCGTTTAATGGCAACG
ATCAGCAGCCGTTGCCAGCGGCTTCCCTTTGTGGCGCCGTCTAAGATGTTGATCAAAAACTGGTTAATTCAGCACTGTCA
GATCCAAGAGGATGTGACTTGGTGTTTGAGTGTGGTCGGCGGCCCATTAAAGTTGGCCGAAAGTTTGCAAACAGATGGAG
CACAACCGAGTCGCTATCAAACTTTGTTAGGTTTTCGCAAAGATTGGGCGCAAAGTTTGTCGTCAGGCTATCTGTGTGCT
AGTTTGTTAACGATAAGTGAACAACAAATCATTGATGCTCTTAAGGTTTTATACTTACTCCTGAGACAAATTTTACTGAA
GTACGGCGACCAAGATGCGTTTGTGCAAGCGCAAATAGGGAACTTGGCGGCGAAAATCATGGGAATGTGTCATAGCTTAA
GTGCAATGCCGAGTGTCAATTATTTAGGCTTGTGTCAAAGTTATGTCTTAGAATATAGAGAAATAACAAATAAGTAA

Upstream 100 bases:

>100_bases
TCAAGCTTGCCAGCGAAGATGAGCGGATTGTTGTCATCGACGCCAGCCAAACCATGGCCGAAGTGCATAAAGATATTCTG
GCCGTGTTACAGGCGATGGC

Downstream 100 bases:

>100_bases
CATGCCATGGTCGATCTTGTCGTCAATTTTGATACCTTACATCAGCTTTACCGCGCCTATATGCCGTTTATTAAACCGGC
AGGGCTGTTTGTCGCCACCG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVAQSGFDVLTLPWLDTPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPAGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGAQPSRYQTLLGFRKDWAQSLSSGYLCA
SLLTISEQQIIDALKVLYLLLRQILLKYGDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK

Sequences:

>Translated_318_residues
MVAQSGFDVLTLPWLDTPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPAGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGAQPSRYQTLLGFRKDWAQSLSSGYLCA
SLLTISEQQIIDALKVLYLLLRQILLKYGDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK
>Mature_318_residues
MVAQSGFDVLTLPWLDTPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPAGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGAQPSRYQTLLGFRKDWAQSLSSGYLCA
SLLTISEQQIIDALKVLYLLLRQILLKYGDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=33.7016574585635, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35073; Mature: 35073

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAQSGFDVLTLPWLDTPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPAGGCGFCKSCQLFDAGNHPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAII
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMATISSRCQRLPFVAPSKMLIKN
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
WLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGAQPSRYQTLLGFRKDWAQSLSSGYLCA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
SLLTISEQQIIDALKVLYLLLRQILLKYGDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCH
YLGLCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAQSGFDVLTLPWLDTPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPAGGCGFCKSCQLFDAGNHPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAII
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMATISSRCQRLPFVAPSKMLIKN
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
WLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGAQPSRYQTLLGFRKDWAQSLSSGYLCA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
SLLTISEQQIIDALKVLYLLLRQILLKYGDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCH
YLGLCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]