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The map label for this gene is lpsJ [H]

Identifier: 117920113

GI number: 117920113

Start: 1957549

End: 1958205

Strand: Reverse

Name: lpsJ [H]

Synonym: Shewana3_1666

Alternate gene names: 117920113

Gene position: 1958205-1957549 (Counterclockwise)

Preceding gene: 117920114

Following gene: 117920112

Centisome position: 39.38

GC content: 49.32

Gene sequence:

>657_bases
ATGGCATTATCAAGAGAACAACTGGCACAGCGCGTTGCCAAAGAATTACAAGACGGCTACTACGTCAACCTCGGTATTGG
CATTCCCACGCTAGTGGCTAACTATATTCCCGCAGGAATGGAAGTGATGCTGCAATCGGAAAACGGCCTGCTCGGCATGG
GGGAATTTCCGACAGAAGAAACCATAGATCCGGATTTAATTAACGCCGGCAAACAAACCGTTACCGCAGTAAAAGGTGCC
TCGTTTTTCTCCTCCGCCGAAAGCTTTGCGATGATCCGTGGCGGCCATGTGGACTTAACCGTACTCGGCGCCTTTGAGGT
CGATGTGAATGGTTCCATCGCCTCATGGATGATCCCAGGTAAGCTTATCAAGGGCATGGGCGGCGCAATGGATTTAGTGG
CGGGCGCGGAAAATATTATCGTGACTATGATGCACGCCGATAAGTACGGTAACTCTAAGCTGTTACCCGTGTGCGAATTG
CCCCTCACAGGTTACGGCTGTATCAAACGTGTTGTTACCGATTTAGCCTTTATCGAGATTAAAGATGGCGCCTTTCATCT
GTTAGAACGCGCACCGGGTGTCAGCGTTGAAGAAATTGTTAGCAAAACCGCTGGCAAATTAATTGTGCCAGACCATGTAC
CAGAGATGGTGTTTTAA

Upstream 100 bases:

>100_bases
ACGATTAACAACGCAAGCTAAAGCGACAGAGTCTAAGCCCTAAATAAAGACCGAAAAATTGAGGCCAATAGCGACTCTAG
GTAAAGGAAGGATAAAGATC

Downstream 100 bases:

>100_bases
GCGTTCTTTAAAATGCGCAAATAACCTATAGCAATAAGGCTTGAAACTTACGTTAAATTGAAGCTTTAGCCTAAAAAGTG
TTGAGCGTTAACCAATATTT

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MALSREQLAQRVAKELQDGYYVNLGIGIPTLVANYIPAGMEVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCEL
PLTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF

Sequences:

>Translated_218_residues
MALSREQLAQRVAKELQDGYYVNLGIGIPTLVANYIPAGMEVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCEL
PLTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
>Mature_217_residues
ALSREQLAQRVAKELQDGYYVNLGIGIPTLVANYIPAGMEVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGAS
FFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPGKLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELP
LTGYGCIKRVVTDLAFIEIKDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=213, Percent_Identity=51.1737089201878, Blast_Score=216, Evalue=9e-57,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=47.906976744186, Blast_Score=210, Evalue=8e-55,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=47.0873786407767, Blast_Score=201, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=53.2407407407407, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=55.607476635514, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=55.607476635514, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23242; Mature: 23111

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSREQLAQRVAKELQDGYYVNLGIGIPTLVANYIPAGMEVMLQSENGLLGMGEFPTEE
CCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHEEEEECCCEEEEEEEEEEEECCCCEEEEECCH
KLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELPLTGYGCIKRVVTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
ALSREQLAQRVAKELQDGYYVNLGIGIPTLVANYIPAGMEVMLQSENGLLGMGEFPTEE
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVNGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHEEEEECCCEEEEEEEEEEEECCCCEEEEECCH
KLIKGMGGAMDLVAGAENIIVTMMHADKYGNSKLLPVCELPLTGYGCIKRVVTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVSVEEIVSKTAGKLIVPDHVPEMVF
CCCHHHHHHCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]