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The map label for this gene is ribE [H]

Identifier: 117919546

GI number: 117919546

Start: 1291560

End: 1292216

Strand: Direct

Name: ribE [H]

Synonym: Shewana3_1097

Alternate gene names: 117919546

Gene position: 1291560-1292216 (Clockwise)

Preceding gene: 117919545

Following gene: 117919547

Centisome position: 25.98

GC content: 50.38

Gene sequence:

>657_bases
ATGTTTACTGGGATTATTGAGGCCGTAGGCACGCTGCGAAAGCTTGAACGTAAAGGCGATGATATTCGTTTGACGGTCGC
CAGTGGCAAACTGGATTTAAGCGATGTGCGTTTAGGCGACAGTATCGCCACCAATGGTGTGTGTTTGACTGTGGTTCAGC
AATTAGCCGACGGCTATGTGGCGGATGTGTCGGCTGAAACTGTCAGTCTCACAGGCTTTGCTAACTATAAAGTGGGCACT
AAGGTTAATCTTGAAAAAGCCGTTACCCCGACAACTCGCCTCGGCGGGCATATGGTCAGCGGCCATGTGGATGGCATTGC
CACCGTAGAGCAGCGTTTAGCCCGGGGCCAAGCAATCGAGTTTTGGTTAGCGGCGCCAACTGAGCTGGCGCGCTATATCG
CCCATAAAGGTTCTATCACCATCGATGGTGTGAGCCTGACGGTAAATGAAGTCGATGGACACCGTTTTCGTTTAACCATA
GTGCCTCATACTGCGGGTGAAACCACACTGGTGGATTTAAAAGCTGGCGATAAGGTGAATATTGAAGTGGATTTAATCGC
CCGCTATTTAGAGCGTTTAATGCGCTTTGATACTAAAGAAACCCAAGGCGGTGGGGTGACCATGGAAATGTTAGCCCGTG
CTGGCTTTGTGCGTTAG

Upstream 100 bases:

>100_bases
CCGACCCTCAAACTGGTCGATGAGCGTAAAGTGGGCGCGGATATGCGTTTCACCTTGCGGCTCACGTCCAATCCATCTTT
AGCGAATAAGTGAGTTAACC

Downstream 100 bases:

>100_bases
GGCACTGGCACTTAGCTCACTTAGGTATAGAATTTCATAACAACAGTAAAATCATAAAGGTCCTACAATGGCGCTGCACA
GTATAGAAGAGATCATCGAG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MFTGIIEAVGTLRKLERKGDDIRLTVASGKLDLSDVRLGDSIATNGVCLTVVQQLADGYVADVSAETVSLTGFANYKVGT
KVNLEKAVTPTTRLGGHMVSGHVDGIATVEQRLARGQAIEFWLAAPTELARYIAHKGSITIDGVSLTVNEVDGHRFRLTI
VPHTAGETTLVDLKAGDKVNIEVDLIARYLERLMRFDTKETQGGGVTMEMLARAGFVR

Sequences:

>Translated_218_residues
MFTGIIEAVGTLRKLERKGDDIRLTVASGKLDLSDVRLGDSIATNGVCLTVVQQLADGYVADVSAETVSLTGFANYKVGT
KVNLEKAVTPTTRLGGHMVSGHVDGIATVEQRLARGQAIEFWLAAPTELARYIAHKGSITIDGVSLTVNEVDGHRFRLTI
VPHTAGETTLVDLKAGDKVNIEVDLIARYLERLMRFDTKETQGGGVTMEMLARAGFVR
>Mature_218_residues
MFTGIIEAVGTLRKLERKGDDIRLTVASGKLDLSDVRLGDSIATNGVCLTVVQQLADGYVADVSAETVSLTGFANYKVGT
KVNLEKAVTPTTRLGGHMVSGHVDGIATVEQRLARGQAIEFWLAAPTELARYIAHKGSITIDGVSLTVNEVDGHRFRLTI
VPHTAGETTLVDLKAGDKVNIEVDLIARYLERLMRFDTKETQGGGVTMEMLARAGFVR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=36.7567567567568, Blast_Score=113, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=39.9014778325123, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23483; Mature: 23483

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEAVGTLRKLERKGDDIRLTVASGKLDLSDVRLGDSIATNGVCLTVVQQLADGYV
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEHHHEECCCCCCCCCHHHHHHHHHHCCEE
ADVSAETVSLTGFANYKVGTKVNLEKAVTPTTRLGGHMVSGHVDGIATVEQRLARGQAIE
EECCCCEEEEEEEECEECCCEEEHHHCCCCCHHCCCEEEECCCCCHHHHHHHHHCCCEEE
FWLAAPTELARYIAHKGSITIDGVSLTVNEVDGHRFRLTIVPHTAGETTLVDLKAGDKVN
EEEECHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCEEE
IEVDLIARYLERLMRFDTKETQGGGVTMEMLARAGFVR
EEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFTGIIEAVGTLRKLERKGDDIRLTVASGKLDLSDVRLGDSIATNGVCLTVVQQLADGYV
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEHHHEECCCCCCCCCHHHHHHHHHHCCEE
ADVSAETVSLTGFANYKVGTKVNLEKAVTPTTRLGGHMVSGHVDGIATVEQRLARGQAIE
EECCCCEEEEEEEECEECCCEEEHHHCCCCCHHCCCEEEECCCCCHHHHHHHHHCCCEEE
FWLAAPTELARYIAHKGSITIDGVSLTVNEVDGHRFRLTIVPHTAGETTLVDLKAGDKVN
EEEECHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCEEE
IEVDLIARYLERLMRFDTKETQGGGVTMEMLARAGFVR
EEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]