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The map label for this gene is cspL

Identifier: 116872795

GI number: 116872795

Start: 1365864

End: 1366064

Strand: Direct

Name: cspL

Synonym: lwe1379

Alternate gene names: 116872795

Gene position: 1365864-1366064 (Clockwise)

Preceding gene: 116872794

Following gene: 116872796

Centisome position: 48.54

GC content: 43.28

Gene sequence:

>201_bases
ATGGAACAAGGTACAGTAAAATGGTTTAACGCAGAAAAAGGATTTGGTTTTATCGAACGCGAAAACGGTGACGATGTATT
CGTACATTTCAGCGCTATCCAAGGCGACGGATTCAAATCTTTAGACGAAGGTCAAGCAGTAACTTTCGACGTTGAAGAAG
GCCAACGCGGACCTCAAGCAGCTAACGTTCAAAAAGCGTAA

Upstream 100 bases:

>100_bases
TGTGGGTGAAAGATACGATTGTGAACAACTTTCCATCTCGTGCCGTTAAGCAAGAATAGTAAATAATTAGTGTGCATAAC
ACACGAGGAGGAACATGAAC

Downstream 100 bases:

>100_bases
TTCTATTTTTTGAATAAGAAAAAGCAAATCATTTCGGTGATTTGCTTTTTTATTTGCCTAAAATCATTTTACCTTGTTTG
GTTTAACTGGAATTATTTGC

Product: cold shock protein

Products: NA

Alternate protein names: CspL

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA

Sequences:

>Translated_66_residues
MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA
>Mature_66_residues
MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=68.8524590163934, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=65.5737704918033, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=63.9344262295082, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=64.5161290322581, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=64.5161290322581, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=54.6875, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=60.655737704918, Blast_Score=75, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSPA_LISIN (P0A356)

Other databases:

- EMBL:   AL596168
- EMBL:   U60042
- PIR:   AH1607
- RefSeq:   NP_470737.1
- ProteinModelPortal:   P0A356
- SMR:   P0A356
- GeneID:   1129998
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin1401
- GenoList:   LIN1401
- HOGENOM:   HBG717773
- OMA:   FITKEGG
- ProtClustDB:   CLSK895002
- BioCyc:   LINN272626:LIN1401-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- PRINTS:   PR00050
- SMART:   SM00357

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7266; Mature: 7266

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQA
CCCCCEEEEECCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCC
ANVQKA
CCCCCC
>Mature Secondary Structure
MEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQA
CCCCCEEEEECCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCC
ANVQKA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003