Click here to switch to the map view.

The map label for this gene is guaA [H]

Identifier: 116872487

GI number: 116872487

Start: 1091959

End: 1093515

Strand: Direct

Name: guaA [H]

Synonym: lwe1071

Alternate gene names: 116872487

Gene position: 1091959-1093515 (Clockwise)

Preceding gene: 116872486

Following gene: 116872488

Centisome position: 38.8

GC content: 42.52

Gene sequence:

>1557_bases
TTGTTTAAAATTATGAAAGACTTTACCGAGCAAGAGAAAATTATCGTTCTAGATTTTGGTAGTCAGTACAATCAACTAAT
TACGCGCCGCATTCGTGAATTCGGTGTGTATAGTGAATTACATCCGCATACTATTACAGTTGAAGAAATGAAAGCACTAA
ATCCAACGGGGATTATTTTTTCAGGAGGACCTAACAGTGTGTATGACAAAGATGCTTTTCGCGCTGACGAAAGAATCTTT
GACATGGGAATTCCGATTTTAGGAATTTGTTACGGCATGCAATTAATGACAACCCATTTCGATGGTAAAGTAGAACGTGC
AAAAGATCGCGAATACGGGAAAGCGGATATTCATGTGGAAAATCCGAGCCGTTTATTTGCAGGACTTCCAACAGATCAAG
TTGTCTGGATGAGTCACGGCGATTTAGTCGTTGAGGAACCAGCTGGTTTTGAAGTAACAGCAACTAGTCCATCTTGCCCA
ATTGCTGGAATTGCAGACGAAGAACGGTTACTATACGGCGTGCAATTCCACCCAGAAGTACGTCACTCTGTTTATGGAAA
CGAATTACTGAAGAATTTTGCATTAAACGTATGTGGATGTAAAGGCGACTGGACAATGGAAAACTTTAGTGAAGTAGAAA
TCGCTAAAATCCAAGAAATCGTTGGTGATAAAAAAGTCTTGCTTGCACTTTCTGGCGGTGTTGATTCTTCCGTTGTTGGT
GTGTTAATTCATAAAGCAATTGGCGACCAACTAACATGTATTTTCGTAGACCACGGCCTTCTTCGTAAAGGGGAAGCTGA
CCAAGTAATGGCGACGTTACAAGGCGAATTCAACATGAACATCATCAAAGTAGATGCGAAAAAACGTTTCATGGACAAAC
TAGCTGGCGTTTCTGACCCAGAACAAAAACGTAAAATCATCGGAAACGAATTCATTTACGTATTCGACGATGAAGCCAAC
AAACTAGATGGCGTAGAATTCCTTGCACAAGGAACACTTTACACAGACATCATCGAAAGTGGTACAGCAACTGCCCAAAC
AATCAAGTCGCACCACAATGTCGGCGGTTTACCAGAAGATATGCAATTTAAACTAATCGAACCATTAAATACACTCTTCA
AAGATGAAGTTCGAGCACTAGGAACTGAACTTGGCATGCCTGACGCAATCGTTTGGCGCCAACCATTCCCAGGCCCAGGC
CTAGGAATCCGCGTCCTTGGCGAAATCACGGAAGAAAAATTAGAGATTGTCCGCGATTCTGATTACATCCTGCGCGAAGA
AATCAAAAAAGCCGGCCTAGAACGCGAAATCTGGCAATATTTCACAGCACTTCCAAACATCCGTAGCGTTGGTGTTATGG
GTGATGGCAGAACGTATGACCATACTGTGGTTGTTCGTGCGGTGACGAGTATTGACGGTATGACAGCTGATTGGGCACGT
ATTCCTTGGGATGTACTGGAGAAGATTTCAGTGCGGATTGTGAATGAAGTGGATCATGTGAATCGTGTTGTTTATGATAT
TACGAGTAAGCCACCAGCTACGGTTGAGTGGGAATAA

Upstream 100 bases:

>100_bases
AAAATGAAAGAAGATGAAAAGGACAAGTGATTTGTCCTTTTCTTATATTATAGTGAAGTAACCGCAATTTTGCGGTTTCA
TTTTATAGATAAGGGTGGTT

Downstream 100 bases:

>100_bases
AAACAAAGCCCTAAAACCTCAGTATTGATGAAGTTTTAGGGCTTTCCCTATGTTGAGTTATAACATTTTTTATTTATCGT
AGTATTTTTACGCATAATTA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDKDAFRADERIF
DMGIPILGICYGMQLMTTHFDGKVERAKDREYGKADIHVENPSRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTATSPSCP
IAGIADEERLLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKKAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE

Sequences:

>Translated_518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDKDAFRADERIF
DMGIPILGICYGMQLMTTHFDGKVERAKDREYGKADIHVENPSRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTATSPSCP
IAGIADEERLLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKKAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
>Mature_518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDKDAFRADERIF
DMGIPILGICYGMQLMTTHFDGKVERAKDREYGKADIHVENPSRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTATSPSCP
IAGIADEERLLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKKAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=546, Percent_Identity=38.4615384615385, Blast_Score=309, Evalue=5e-84,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=50.8571428571429, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789760, Length=154, Percent_Identity=39.6103896103896, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992717, Length=567, Percent_Identity=37.7425044091711, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI71992710, Length=567, Percent_Identity=37.7425044091711, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI133901714, Length=567, Percent_Identity=37.7425044091711, Blast_Score=330, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6323873, Length=516, Percent_Identity=54.2635658914729, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322638, Length=186, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365319, Length=548, Percent_Identity=36.3138686131387, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 58054; Mature: 58054

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIF
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEE
SGGPNSVYDKDAFRADERIFDMGIPILGICYGMQLMTTHFDGKVERAKDREYGKADIHVE
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCHHHCCCCCCCCEEEEEC
NPSRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTATSPSCPIAGIADEERLLYGVQFHPEV
CCHHHEECCCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCHH
RHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDP
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCCCH
EQKRKIIGNEFIYVFDDEANKLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPED
HHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPGLGIRVLGEITEEKLEIVRDS
CCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DYILREEIKKAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
CHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCCCCCCCHHH
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIF
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEE
SGGPNSVYDKDAFRADERIFDMGIPILGICYGMQLMTTHFDGKVERAKDREYGKADIHVE
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCHHHCCCCCCCCEEEEEC
NPSRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTATSPSCPIAGIADEERLLYGVQFHPEV
CCHHHEECCCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCHH
RHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDP
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCCCCH
EQKRKIIGNEFIYVFDDEANKLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPED
HHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPGLGIRVLGEITEEKLEIVRDS
CCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DYILREEIKKAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
CHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCCCCCCCHHH
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]