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The map label for this gene is cwlC [H]

Identifier: 116871531

GI number: 116871531

Start: 111760

End: 112488

Strand: Direct

Name: cwlC [H]

Synonym: lwe0111

Alternate gene names: 116871531

Gene position: 111760-112488 (Clockwise)

Preceding gene: 116871530

Following gene: 116871533

Centisome position: 3.97

GC content: 35.12

Gene sequence:

>729_bases
ATGACAAAAATATGGATTGATGCAGGTCATGGTGGGAAAGATTCTGGCGCAGTTGGAAACGGGCTTGTCGAAAAAGATTG
GGTGCTGACTGTAGCGAAACAAATTCAAAATGAATTGATGAAAGCTGGTTTTGAAGTTGGTATGACACGAACAAATGATA
CTTTTTATGAATTAAGTGATCGTGCTAAAAAAGCAAATAGTTTCAAAGCGGATTTATTTATTTCGATTCACTTTAATGCT
GGTGGGGGAAAAGGTTATGAAGACTATATTTATACGTCTACTCCCGCGAAAACAGTTGAAATACAAAAAATTATCCACAA
AAATGTTATCTCTAAAATTACTAAACATGGAATGAACGACCGAGGAATAAAGAAAGCTAATTTTGCTGTTTTAAGAGAAA
CGGCAATGGATGCAATTTTACTTGAGGCTGGTTTTTGTGATAGTACAGACGCGGCTATTCTTGAAAAACAATCCTATAAA
AATGATTATTGTTCAGGTATTGTTGCAGGCGTTCAGGAGATTTTTGGAGCTATGGTTACAAAATATCGTGCAGGAAAATA
TTTGACTAGCGACGATGCTATTTCTGGCAACAATATCAAAGGTTATTTGGAAGCAGGAACGAAGGTTTTTGTTTATAAAG
AAACAGACAAAACGCTTAATTTAACTACGAAAAAAGGTGTACCAGGAAGTTGGGTTCTAAAAACAGAAGTGAATATTGGA
AAGAGATAA

Upstream 100 bases:

>100_bases
ATTTTGGAAAATTTTGTTCGAATGGGGATGAAAGTCCCGGAAATATTGAAAAATTTAATTTTAATTTTTGATGCAAAAGC
AGGAGATGAGGAGGAAAAGC

Downstream 100 bases:

>100_bases
AAAGAGCGACCAATTTGGTCGCTCCTTTTTAATCAACCCTTTTTACGTAAAGTATAAAGAGCTGTAGTTGTTAAAATAAG
ACCGAATACTGTTGCAAGTC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTKIWIDAGHGGKDSGAVGNGLVEKDWVLTVAKQIQNELMKAGFEVGMTRTNDTFYELSDRAKKANSFKADLFISIHFNA
GGGKGYEDYIYTSTPAKTVEIQKIIHKNVISKITKHGMNDRGIKKANFAVLRETAMDAILLEAGFCDSTDAAILEKQSYK
NDYCSGIVAGVQEIFGAMVTKYRAGKYLTSDDAISGNNIKGYLEAGTKVFVYKETDKTLNLTTKKGVPGSWVLKTEVNIG
KR

Sequences:

>Translated_242_residues
MTKIWIDAGHGGKDSGAVGNGLVEKDWVLTVAKQIQNELMKAGFEVGMTRTNDTFYELSDRAKKANSFKADLFISIHFNA
GGGKGYEDYIYTSTPAKTVEIQKIIHKNVISKITKHGMNDRGIKKANFAVLRETAMDAILLEAGFCDSTDAAILEKQSYK
NDYCSGIVAGVQEIFGAMVTKYRAGKYLTSDDAISGNNIKGYLEAGTKVFVYKETDKTLNLTTKKGVPGSWVLKTEVNIG
KR
>Mature_241_residues
TKIWIDAGHGGKDSGAVGNGLVEKDWVLTVAKQIQNELMKAGFEVGMTRTNDTFYELSDRAKKANSFKADLFISIHFNAG
GGKGYEDYIYTSTPAKTVEIQKIIHKNVISKITKHGMNDRGIKKANFAVLRETAMDAILLEAGFCDSTDAAILEKQSYKN
DYCSGIVAGVQEIFGAMVTKYRAGKYLTSDDAISGNNIKGYLEAGTKVFVYKETDKTLNLTTKKGVPGSWVLKTEVNIGK
R

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze typ

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall. Note=Is associated with the mother cell wall of sporulating cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=231, Percent_Identity=29.4372294372294, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788776, Length=218, Percent_Identity=25.2293577981651, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26530; Mature: 26398

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIWIDAGHGGKDSGAVGNGLVEKDWVLTVAKQIQNELMKAGFEVGMTRTNDTFYELSD
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RAKKANSFKADLFISIHFNAGGGKGYEDYIYTSTPAKTVEIQKIIHKNVISKITKHGMND
HHHHCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
RGIKKANFAVLRETAMDAILLEAGFCDSTDAAILEKQSYKNDYCSGIVAGVQEIFGAMVT
CCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KYRAGKYLTSDDAISGNNIKGYLEAGTKVFVYKETDKTLNLTTKKGVPGSWVLKTEVNIG
HHHCCCEECCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
KR
CC
>Mature Secondary Structure 
TKIWIDAGHGGKDSGAVGNGLVEKDWVLTVAKQIQNELMKAGFEVGMTRTNDTFYELSD
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RAKKANSFKADLFISIHFNAGGGKGYEDYIYTSTPAKTVEIQKIIHKNVISKITKHGMND
HHHHCCCCEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
RGIKKANFAVLRETAMDAILLEAGFCDSTDAAILEKQSYKNDYCSGIVAGVQEIFGAMVT
CCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KYRAGKYLTSDDAISGNNIKGYLEAGTKVFVYKETDKTLNLTTKKGVPGSWVLKTEVNIG
HHHCCCEECCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407798; 9384377 [H]