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The map label for this gene is ykoW [H]

Identifier: 116871533

GI number: 116871533

Start: 115100

End: 115849

Strand: Direct

Name: ykoW [H]

Synonym: lwe0113

Alternate gene names: 116871533

Gene position: 115100-115849 (Clockwise)

Preceding gene: 116871531

Following gene: 116871535

Centisome position: 4.09

GC content: 36.4

Gene sequence:

>750_bases
ATGGATATCTCGAGCACGGAAATTTGGGATGCGATTAGGAGAAATAGTTATTTACTATATTATCAACCAAAAGTAGATGC
TAAAACAAATAAAGTCATTGGTTTTGAAGGCTTAATCAGGTTGAAAACAGCGACAGCAGTTCTGGCCCCAATGGATTTCT
TTGAGGATGTTGCCCTTTTAAATGCAACTCGGGAAATGCAAGATTTTGTTGCTGAAACGGCGATAGAACAGCTTAATCAG
TTGGGTGGGCGTTTTTCTATTTCGATTAATATTCCAGCACATTATGTAGTAAGCAGTAATTATATGGCTTCTTTGTATGA
TTATGTGAAAGAACATTTAAAGTATCCGGAATGCTTAGAAATAGAAATTATTGAGCGAGGCGAGATTACGGAACTTGCTG
TTGCAGACAAAAACTTAAGAAAAATCAAAAATCTTGGTGTCAAGGTGAGCATGGATGATTTTGGAAAAGGTTATAGTTCG
CTTGCCTATTTGCGCAGTTTGCCAATTGATATTGTCAAAACAGATATGTCGTTTATTGCGCTTTTAAAAACAGATCGTCG
ACAACAAATAATTATTCAAGCAATTGTTAACTTATGCCATGATTTAGGTGGGAAAGTGGTCACGGAAGGCGTAGAAGACA
TTGAACAAGTGAAAAAATTACGAGAAATGAAAGTGGACTACTTTCAAGGCTTTTATTTTAGTCGGCCGCTACCAATGGAA
GAAATCAAACAAAAATATTCCATCGTCTGA

Upstream 100 bases:

>100_bases
GCAATAGGAAATATGGATTTTGTGTAAATGTAATGATAATATTTGGCTATATTTTTATTTCGTTTGAAAAAAGGGTAAAC
TATAGAAAAGGAGGGATAGG

Downstream 100 bases:

>100_bases
ATAGAAAATTGGTTGGTGTGAGAATGGCAATTTACCAAATTCACACCAACCAATTTTATTTTTATTTAATTAAATTATTT
GAAGCATCTTTTACAACAAC

Product: EAL domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKVIGFEGLIRLKTATAVLAPMDFFEDVALLNATREMQDFVAETAIEQLNQ
LGGRFSISINIPAHYVVSSNYMASLYDYVKEHLKYPECLEIEIIERGEITELAVADKNLRKIKNLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRRQQIIIQAIVNLCHDLGGKVVTEGVEDIEQVKKLREMKVDYFQGFYFSRPLPME
EIKQKYSIV

Sequences:

>Translated_249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKVIGFEGLIRLKTATAVLAPMDFFEDVALLNATREMQDFVAETAIEQLNQ
LGGRFSISINIPAHYVVSSNYMASLYDYVKEHLKYPECLEIEIIERGEITELAVADKNLRKIKNLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRRQQIIIQAIVNLCHDLGGKVVTEGVEDIEQVKKLREMKVDYFQGFYFSRPLPME
EIKQKYSIV
>Mature_249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKVIGFEGLIRLKTATAVLAPMDFFEDVALLNATREMQDFVAETAIEQLNQ
LGGRFSISINIPAHYVVSSNYMASLYDYVKEHLKYPECLEIEIIERGEITELAVADKNLRKIKNLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRRQQIIIQAIVNLCHDLGGKVVTEGVEDIEQVKKLREMKVDYFQGFYFSRPLPME
EIKQKYSIV

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790496, Length=243, Percent_Identity=30.8641975308642, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=30.1587301587302, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87081921, Length=247, Percent_Identity=32.7935222672065, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI226510982, Length=248, Percent_Identity=28.2258064516129, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081743, Length=236, Percent_Identity=28.3898305084746, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787541, Length=246, Percent_Identity=26.0162601626016, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081845, Length=232, Percent_Identity=27.1551724137931, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786507, Length=233, Percent_Identity=26.6094420600858, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081980, Length=248, Percent_Identity=28.2258064516129, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082096, Length=237, Percent_Identity=26.5822784810127, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787055, Length=237, Percent_Identity=27.4261603375527, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788849, Length=234, Percent_Identity=24.3589743589744, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787410, Length=218, Percent_Identity=25.2293577981651, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 28511; Mature: 28511

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKVIGFEGLIRLKTATAVLAPMDFFEDVALL
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
NATREMQDFVAETAIEQLNQLGGRFSISINIPAHYVVSSNYMASLYDYVKEHLKYPECLE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEE
IEIIERGEITELAVADKNLRKIKNLGVKVSMDDFGKGYSSLAYLRSLPIDIVKTDMSFIA
EEEECCCCEEHHHHHHHHHHHHHHCCCEEEHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
LLKTDRRQQIIIQAIVNLCHDLGGKVVTEGVEDIEQVKKLREMKVDYFQGFYFSRPLPME
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIKQKYSIV
HHHHHHCCC
>Mature Secondary Structure
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKVIGFEGLIRLKTATAVLAPMDFFEDVALL
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
NATREMQDFVAETAIEQLNQLGGRFSISINIPAHYVVSSNYMASLYDYVKEHLKYPECLE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEE
IEIIERGEITELAVADKNLRKIKNLGVKVSMDDFGKGYSSLAYLRSLPIDIVKTDMSFIA
EEEECCCCEEHHHHHHHHHHHHHHCCCEEEHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
LLKTDRRQQIIIQAIVNLCHDLGGKVVTEGVEDIEQVKKLREMKVDYFQGFYFSRPLPME
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIKQKYSIV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]