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The map label for this gene is purA [H]

Identifier: 116871468

GI number: 116871468

Start: 45115

End: 46407

Strand: Direct

Name: purA [H]

Synonym: lwe0046

Alternate gene names: 116871468

Gene position: 45115-46407 (Clockwise)

Preceding gene: 116871467

Following gene: 116871469

Centisome position: 1.6

GC content: 37.97

Gene sequence:

>1293_bases
ATGTCTTCAGTTGTTGTAGTAGGAACACAGTGGGGCGATGAAGGAAAAGGGAAGATTACGGATTTTCTTTCCGAGAATGC
AGAAGCGATTGCTAGATATCAAGGTGGGAACAATGCAGGTCATACAATTAAGTTTGATGGCGAAACGTACAAATTGCATT
TAATTCCATCCGGTATCTTTTACAAAGAAAAAATCAGTGTTATTGGTAATGGTATGGTTGTAGATCCAAAGGCTTTAGTG
GAGGAATTAAAATATCTTCATGACAAAGGCGTGGATACTTCTAATTTGCGTATTTCTAACCGCGCACACATTATTCTGCC
GTATCACATTCGTATCGATGAAGCTGATGAAGAACGTAAAGGCGCAAATAAAATCGGTACAACGAAAAAAGGTATCGGCC
CAGCATATATGGATAAAGCGGCTCGTGTCGGTATTCGTATTATTGATTTATTAGATAAAGAAACATTTAAAGAAAAATTA
GAGCATAATCTGGGAGAAAAAAACCGTTTACTTGAACGTTTTTATGAACTAGAAGGCTTTAAATTAGAAGATATTTTAGA
AGAGTATTATGGTTATGGTCAACAATTTAAAGATTATGTTTGTGATACATCGGTTGTGTTGAATGATGCACTTGATGATG
GCAAACGTGTATTATTTGAAGGTGCGCAAGGTGTTATGCTTGATATTGATCAAGGGACATATCCATTTGTAACTTCAAGT
AACCCAATTGCTGGTGGTGTAACTATTGGTAGTGGAGTTGGTCCATCAAAAATCAATCATGTTGTTGGTGTGGCAAAAGC
TTATACAACTCGTGTTGGCGACGGTCCTTTCCCAACAGAATTATTTGATTCTATTGGTGACAATATTCGTGAAGTTGGTC
GTGAATATGGTACAACTACTGGTCGTCCGCGTCGTGTAGGTTGGTTTGATAGTGTTGTTGTCCGTCATGCTCGTCGTGTT
AGTGGCTTAACTGATTTATCATTAACACTACTCGATGTTTTGACAGGAATTGAAACACTTAAAATCTGTGTAGCATATAA
GTTAGATGGAAAAACAATTACTGAATTCCCAGCAAGCTTGAAAGACTTAGCTCGTTGCGAACCAGTCTATGAAGAATTAC
CAGGTTGGACAGAAGACATTACAGAAGTTAAATCATTAGATGATCTTCCAGTAAATTGTCGTCACTATATGGAACGTATT
GCGCAACTTACAGGTGTACAAGTTTCTATGTTCTCTGTTGGTCCTGACCGCGCTCAAACACATGTGGTTAAAAGTGTGTG
GCGTTTAGCTTAA

Upstream 100 bases:

>100_bases
ATTGACTATTTTGACGGCAAACGGTACAATATATAATGGAAAATGAAGAGGTTATGAAAAAAATCATAGCTATCAAAGAC
CAATTAAATGAGGTGTTTAA

Downstream 100 bases:

>100_bases
TAATAAGGTTTAAAGCTTCTTGAGGTATTTTTCTCAAGAAGCTTTTTTTGTTTCACGTGAAACATTTTGATTAAATAATG
GCAGTTTATCTTATGAGGAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGETYKLHLIPSGIFYKEKISVIGNGMVVDPKALV
EELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKL
EHNLGEKNRLLERFYELEGFKLEDILEEYYGYGQQFKDYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDSIGDNIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITEVKSLDDLPVNCRHYMERI
AQLTGVQVSMFSVGPDRAQTHVVKSVWRLA

Sequences:

>Translated_430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGETYKLHLIPSGIFYKEKISVIGNGMVVDPKALV
EELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKL
EHNLGEKNRLLERFYELEGFKLEDILEEYYGYGQQFKDYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDSIGDNIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITEVKSLDDLPVNCRHYMERI
AQLTGVQVSMFSVGPDRAQTHVVKSVWRLA
>Mature_429_residues
SSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGETYKLHLIPSGIFYKEKISVIGNGMVVDPKALVE
ELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLE
HNLGEKNRLLERFYELEGFKLEDILEEYYGYGQQFKDYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDSIGDNIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITEVKSLDDLPVNCRHYMERIA
QLTGVQVSMFSVGPDRAQTHVVKSVWRLA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=45, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=43.1279620853081, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=43.7340153452685, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=48.9411764705882, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=42.7230046948357, Blast_Score=324, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=41.7452830188679, Blast_Score=332, Evalue=5e-92,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=43.4052757793765, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47681; Mature: 47550

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGETYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCE
YKEKISVIGNGMVVDPKALVEELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERK
EHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLEHNLGEKNRLLERFYELEGF
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
KLEDILEEYYGYGQQFKDYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDSIGDNIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHCCHHH
KDLARCEPVYEELPGWTEDITEVKSLDDLPVNCRHYMERIAQLTGVQVSMFSVGPDRAQT
HHHHHCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
HVVKSVWRLA
HHHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGETYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCE
YKEKISVIGNGMVVDPKALVEELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERK
EHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLEHNLGEKNRLLERFYELEGF
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
KLEDILEEYYGYGQQFKDYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDSIGDNIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHCCHHH
KDLARCEPVYEELPGWTEDITEVKSLDDLPVNCRHYMERIAQLTGVQVSMFSVGPDRAQT
HHHHHCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
HVVKSVWRLA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]