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The map label for this gene is pcaR [H]

Identifier: 116696481

GI number: 116696481

Start: 2872687

End: 2873535

Strand: Direct

Name: pcaR [H]

Synonym: H16_B2545

Alternate gene names: 116696481

Gene position: 2872687-2873535 (Clockwise)

Preceding gene: 116696480

Following gene: 116696484

Centisome position: 98.63

GC content: 69.26

Gene sequence:

>849_bases
ATGGACGTCCTCCGCCCCCTCGGTCTTGGCCATGCACAGGCCGACACCGTCCGCGTTGCCCGGCCCCAGGCCGACCTGCT
CGCCAGTTTTGCCGGCGATCCCAACTTCATGCTGTCGCTGGCGCGCGGGCTGACCGTGCTGGAGGCGTTCTCGGAACGCA
AGCGCCCGCTGACGATCTCGCAGGTGGCGCAGCGCACGCAGCTGTCGCGCGCCTCGGTGCGGCGCTGCCTCTATACGCTG
GAACAGCTCGGCTACGTCAGCCAGCAGGACGGCCAGTTCGCGCTGCGGCCGCGCGTGCTGCACCTGGGCCACGCGTATTT
CTCGTCGACCTCGCTGGTGTCTCTGGCGCAGCCGATCCTGGACAACCTGAGCGCGCGCATCCACGAGACCTGCACGCTGG
CGATCCTGGACGGCACCGACATCCTGTACCTGGTGCGCTCCGAAGTGCAGCGGGTGCTGAACTATTCGCTAGGCATGGGC
AGCCGCCTGCCGGCCTACTGCACCTCCAACGGCCGGCTGCTGCTGGCGCACCAGCCCGCCACGGTGCTGGACGGCTTCTT
CGAGCACGCCGAACTGCGCCCGCGCACGCTGCAGACCAAGGTCTCGCGCCAGGAGCTGGAAGCATGCTTCGAGCGCGCCC
GCGAAGTGGACTACGTGATCGTCGATGAGGAGCTGGAACCGGGCCTGCGCGCGATGGCCGTCCCGGTGCGCTCGGCCTCG
GGCATCGTGCTGGCGGGGCTGAGCGTGAGCGTGCGCGCCGCGCGTGTCTCTGAAACAGAGATGATCTCGCGGCTGCTGCC
GCCGATACGCGAAGCCGCGGCGGCAATTGGCCGGCTGATCGGCTCCTGA

Upstream 100 bases:

>100_bases
TGCGGGCGATGCGCGCATGCAGCAAAGGCTGGCCCCTGGCGGCCAGCCGGTTTAAGATTGCGCGATTATCGGACAACAAG
CCCAGACAACACACCTCCCA

Downstream 100 bases:

>100_bases
TTGCCGCTGACTACCCGGGCAGCCGCACGGGATTGAGGTAAGCCGGGTCCGGGTTGCGCGGCCTCGGCACCAGTGCTTCA
GCCCCGGTGCGCCAGTTCCA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDVLRPLGLGHAQADTVRVARPQADLLASFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSLVSLAQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVLNYSLGMG
SRLPAYCTSNGRLLLAHQPATVLDGFFEHAELRPRTLQTKVSRQELEACFERAREVDYVIVDEELEPGLRAMAVPVRSAS
GIVLAGLSVSVRAARVSETEMISRLLPPIREAAAAIGRLIGS

Sequences:

>Translated_282_residues
MDVLRPLGLGHAQADTVRVARPQADLLASFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSLVSLAQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVLNYSLGMG
SRLPAYCTSNGRLLLAHQPATVLDGFFEHAELRPRTLQTKVSRQELEACFERAREVDYVIVDEELEPGLRAMAVPVRSAS
GIVLAGLSVSVRAARVSETEMISRLLPPIREAAAAIGRLIGS
>Mature_282_residues
MDVLRPLGLGHAQADTVRVARPQADLLASFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSLVSLAQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVLNYSLGMG
SRLPAYCTSNGRLLLAHQPATVLDGFFEHAELRPRTLQTKVSRQELEACFERAREVDYVIVDEELEPGLRAMAVPVRSAS
GIVLAGLSVSVRAARVSETEMISRLLPPIREAAAAIGRLIGS

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=30.4878048780488, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788131, Length=255, Percent_Identity=30.5882352941176, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082359, Length=248, Percent_Identity=27.0161290322581, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789999, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786715, Length=228, Percent_Identity=25.8771929824561, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30945; Mature: 30945

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLRPLGLGHAQADTVRVARPQADLLASFAGDPNFMLSLARGLTVLEAFSERKRPLTIS
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVAQRTQLSRASVRRCLYTLEQLGYVSQQDGQFALRPRVLHLGHAYFSSTSLVSLAQPIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
DNLSARIHETCTLAILDGTDILYLVRSEVQRVLNYSLGMGSRLPAYCTSNGRLLLAHQPA
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCH
TVLDGFFEHAELRPRTLQTKVSRQELEACFERAREVDYVIVDEELEPGLRAMAVPVRSAS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHEEEECCCCC
GIVLAGLSVSVRAARVSETEMISRLLPPIREAAAAIGRLIGS
CEEEECCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVLRPLGLGHAQADTVRVARPQADLLASFAGDPNFMLSLARGLTVLEAFSERKRPLTIS
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVAQRTQLSRASVRRCLYTLEQLGYVSQQDGQFALRPRVLHLGHAYFSSTSLVSLAQPIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
DNLSARIHETCTLAILDGTDILYLVRSEVQRVLNYSLGMGSRLPAYCTSNGRLLLAHQPA
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCH
TVLDGFFEHAELRPRTLQTKVSRQELEACFERAREVDYVIVDEELEPGLRAMAVPVRSAS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHEEEECCCCC
GIVLAGLSVSVRAARVSETEMISRLLPPIREAAAAIGRLIGS
CEEEECCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]