Click here to switch to the map view.

The map label for this gene is nirJ [H]

Identifier: 116696219

GI number: 116696219

Start: 2581973

End: 2583136

Strand: Direct

Name: nirJ [H]

Synonym: H16_B2283

Alternate gene names: 116696219

Gene position: 2581973-2583136 (Clockwise)

Preceding gene: 116696218

Following gene: 116696220

Centisome position: 88.65

GC content: 70.45

Gene sequence:

>1164_bases
ATGTTCCGCCTGTCCCGCTTCATGGAAGCCCTGCGCGATGCCGGCCCGGTGCCGCCGCCGCGCAAGCCCGCCGGCCCGGT
GGTGATCTGGAACCTGATCCGCCGCTGCAACCTCAACTGCCGGCACTGCTATGCAACCTCCGCCGACACGGACTTCAAGG
GCGAGCTGGATACCGCCGAGGCGCTGCGCGTGCTCGACCAGTTGCGCGATGCGCGCGTGCCCGCGCTGATCCTGTCCGGG
GGCGAACCGCTGCTGCGGCCGGACCTCTACGAGATTGCCGCACGGGCGCGCGCACTCGGCTTCCACCTGTCGCTGTCGTC
CAACGGCACGCTGCTCGATGCGGGGCATGCCGCGCGGCTAGCGGCAGCGGGCTTCGACTACGTCGGCGTCAGCCTGGACG
GCCTGCCCGCCACGCACGACCGCTTCCGCCGCAGCGACGGCGCCTTTGCGCAGGCGCTGGCGGGCCTGCGCGCCGCGCGT
GCGGCCGGCCTGCGCGTGGGCGTGCGCATGACGCTGACCGAAGCCAACGCGGCGCAACTGCCCGACCTGGTGGCGCTGAC
CGAGCATGAGGGCATCGACAAGTTCTACCTGTCGCACTTCAACTATGCTGGCCGTGCACGCAGCCATCGCGCCGAAGACG
CGCACCATGCGCGCACCCGCGCCGCGCTCGACTGGCTGTTCGACCACGTCTGGCAGCGCACGCAGCAGGGCCATGCGGGC
GACTTCGTCACCGGCAACAACGATGCGGATGGCGTGTACCTGCTGTACTGGATCGCCAGGCGCTTGCCGCATCGCCTGGT
GGATATGCGCCAGCGGCTGGAACACTGGGGCGGCAACGCCACCGGGGTCGGCGTGGCCAATATCGACAACCTCGGCAATG
TCCATCCCGACACGATGTGGTGGCACGTGACCGTGGGCAATGTGCGCGAGCGGCCGTTCGGCGAGATCTGGGCCAGCCGC
GCCGATCCGCTGATGGCGGGCCTGGCCAGCACGCCGCGTCCGCTGCAGGGGCGTTGCGCGGGCTGCGCATATCGCGCCAT
CTGCAACGGCAATACCCGGGTGCGCGCCTTTGCGCTGACAGGCAACGCCTGGGCGGAAGATCCGGGCTGCTACCTGAGCG
ATGCCGAGATCGCGCATGCGCCGGGCACGGAGGTGGCGGCATGA

Upstream 100 bases:

>100_bases
CGCCTGCCGCGCGCACGACGTGCTGTGGTCCACCCGCATCCTCAAGAAAACGGGGCTGCGCCTGCCGCCGCCCGGCCCAG
ACCAATAGGAAACCCCGCCC

Downstream 100 bases:

>100_bases
AGACCGTGCTCCGATGGCTGGTCGGCCTGGCCTTTGCCTGCGCCACGCAAGCCGCCATGGCCGCCGACCCCGCGGCGCTC
TACAGCCAGCACTGCGCCGC

Product: heme d1 biosynthesis protein (NirJ)

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFRLSRFMEALRDAGPVPPPRKPAGPVVIWNLIRRCNLNCRHCYATSADTDFKGELDTAEALRVLDQLRDARVPALILSG
GEPLLRPDLYEIAARARALGFHLSLSSNGTLLDAGHAARLAAAGFDYVGVSLDGLPATHDRFRRSDGAFAQALAGLRAAR
AAGLRVGVRMTLTEANAAQLPDLVALTEHEGIDKFYLSHFNYAGRARSHRAEDAHHARTRAALDWLFDHVWQRTQQGHAG
DFVTGNNDADGVYLLYWIARRLPHRLVDMRQRLEHWGGNATGVGVANIDNLGNVHPDTMWWHVTVGNVRERPFGEIWASR
ADPLMAGLASTPRPLQGRCAGCAYRAICNGNTRVRAFALTGNAWAEDPGCYLSDAEIAHAPGTEVAA

Sequences:

>Translated_387_residues
MFRLSRFMEALRDAGPVPPPRKPAGPVVIWNLIRRCNLNCRHCYATSADTDFKGELDTAEALRVLDQLRDARVPALILSG
GEPLLRPDLYEIAARARALGFHLSLSSNGTLLDAGHAARLAAAGFDYVGVSLDGLPATHDRFRRSDGAFAQALAGLRAAR
AAGLRVGVRMTLTEANAAQLPDLVALTEHEGIDKFYLSHFNYAGRARSHRAEDAHHARTRAALDWLFDHVWQRTQQGHAG
DFVTGNNDADGVYLLYWIARRLPHRLVDMRQRLEHWGGNATGVGVANIDNLGNVHPDTMWWHVTVGNVRERPFGEIWASR
ADPLMAGLASTPRPLQGRCAGCAYRAICNGNTRVRAFALTGNAWAEDPGCYLSDAEIAHAPGTEVAA
>Mature_387_residues
MFRLSRFMEALRDAGPVPPPRKPAGPVVIWNLIRRCNLNCRHCYATSADTDFKGELDTAEALRVLDQLRDARVPALILSG
GEPLLRPDLYEIAARARALGFHLSLSSNGTLLDAGHAARLAAAGFDYVGVSLDGLPATHDRFRRSDGAFAQALAGLRAAR
AAGLRVGVRMTLTEANAAQLPDLVALTEHEGIDKFYLSHFNYAGRARSHRAEDAHHARTRAALDWLFDHVWQRTQQGHAG
DFVTGNNDADGVYLLYWIARRLPHRLVDMRQRLEHWGGNATGVGVANIDNLGNVHPDTMWWHVTVGNVRERPFGEIWASR
ADPLMAGLASTPRPLQGRCAGCAYRAICNGNTRVRAFALTGNAWAEDPGCYLSDAEIAHAPGTEVAA

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42315; Mature: 42315

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLSRFMEALRDAGPVPPPRKPAGPVVIWNLIRRCNLNCRHCYATSADTDFKGELDTAE
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
ALRVLDQLRDARVPALILSGGEPLLRPDLYEIAARARALGFHLSLSSNGTLLDAGHAARL
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHH
AAAGFDYVGVSLDGLPATHDRFRRSDGAFAQALAGLRAARAAGLRVGVRMTLTEANAAQL
HHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCHHHC
PDLVALTEHEGIDKFYLSHFNYAGRARSHRAEDAHHARTRAALDWLFDHVWQRTQQGHAG
CCCEEECCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DFVTGNNDADGVYLLYWIARRLPHRLVDMRQRLEHWGGNATGVGVANIDNLGNVHPDTMW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEE
WHVTVGNVRERPFGEIWASRADPLMAGLASTPRPLQGRCAGCAYRAICNGNTRVRAFALT
EEEEECCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEEEEEE
GNAWAEDPGCYLSDAEIAHAPGTEVAA
CCCCCCCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFRLSRFMEALRDAGPVPPPRKPAGPVVIWNLIRRCNLNCRHCYATSADTDFKGELDTAE
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
ALRVLDQLRDARVPALILSGGEPLLRPDLYEIAARARALGFHLSLSSNGTLLDAGHAARL
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHH
AAAGFDYVGVSLDGLPATHDRFRRSDGAFAQALAGLRAARAAGLRVGVRMTLTEANAAQL
HHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCHHHC
PDLVALTEHEGIDKFYLSHFNYAGRARSHRAEDAHHARTRAALDWLFDHVWQRTQQGHAG
CCCEEECCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DFVTGNNDADGVYLLYWIARRLPHRLVDMRQRLEHWGGNATGVGVANIDNLGNVHPDTMW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEE
WHVTVGNVRERPFGEIWASRADPLMAGLASTPRPLQGRCAGCAYRAICNGNTRVRAFALT
EEEEECCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEEEEEE
GNAWAEDPGCYLSDAEIAHAPGTEVAA
CCCCCCCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11111029 [H]