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The map label for this gene is leuD5 [H]

Identifier: 116696212

GI number: 116696212

Start: 2575918

End: 2576562

Strand: Direct

Name: leuD5 [H]

Synonym: H16_B2276

Alternate gene names: 116696212

Gene position: 2575918-2576562 (Clockwise)

Preceding gene: 116696211

Following gene: 116696213

Centisome position: 88.44

GC content: 66.67

Gene sequence:

>645_bases
ATGCCTGAACCCACCTGCATCGCCGGCAAAGGCGCCGCCATCCGCTTCGAGAACCTCGATACCGACCAGATCATCCCCAA
GCAGTTCCTGCGCGGGATCGACAAGTCGGGGCTGGCCGAGGGCATTCTCTACGACCACCGCTTCGACGCCAGCGGCGCGC
CGCGCCCTGGCTTCGTGCTGAACCGGCCCGAGTACGCCGGCGCCAGCATCCTGGTGAGCGGCGCCAACTTCGGCTGCGGC
TCCAGCCGCGAGCACGCCGTCTGGGGCCTGCAGCAATTCGGCATCCGCGCCGTGATTGCGCCGAGCTTCGGCGAGATCTT
CTATTCGAACGCGATGAACAACCGGCTGCTGCTGGTGATGCTGCCACGCGAGGTGATCGGGCCGTTGATGAACGACGTGG
ATGCGGACGCGGCCAACACCATCAGCATCGATGTCGAGGCGATGCGCGTGCGCACGCCCTCCGGCGACTACGCCTTCGAG
CTGTCGGCACGGCACCGCCGCATGTTTATCGAAGGGCTGGACATGATCGGCCTGTCGCTGCGGCAGCGCGGCGAGATCGA
GCAGTTCATCCAGCAATACCGGAGCGCGTTCCCGTGGACAACGAACGTGGCCAGGCGCACGCGCGACCGCCTGAGCGCGA
CCTGA

Upstream 100 bases:

>100_bases
CAGGGGCGCGGTGCCATCACCCACCTGATGAGCCCGGCCATGGCCGCTGCGGCCGCCGTCACCGGCGTCATCACCGACGT
CCGCAAGCTGGAGATCACCC

Downstream 100 bases:

>100_bases
AGCCTGCCCTGCAAGCGCGTCGCCTCCGCGGCGCGCTTGCCCCGCTGCAAACGACAAGGATCAACACCACCCCACACTTC
GTTCCCGCACCGCCGCCACC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPEPTCIAGKGAAIRFENLDTDQIIPKQFLRGIDKSGLAEGILYDHRFDASGAPRPGFVLNRPEYAGASILVSGANFGCG
SSREHAVWGLQQFGIRAVIAPSFGEIFYSNAMNNRLLLVMLPREVIGPLMNDVDADAANTISIDVEAMRVRTPSGDYAFE
LSARHRRMFIEGLDMIGLSLRQRGEIEQFIQQYRSAFPWTTNVARRTRDRLSAT

Sequences:

>Translated_214_residues
MPEPTCIAGKGAAIRFENLDTDQIIPKQFLRGIDKSGLAEGILYDHRFDASGAPRPGFVLNRPEYAGASILVSGANFGCG
SSREHAVWGLQQFGIRAVIAPSFGEIFYSNAMNNRLLLVMLPREVIGPLMNDVDADAANTISIDVEAMRVRTPSGDYAFE
LSARHRRMFIEGLDMIGLSLRQRGEIEQFIQQYRSAFPWTTNVARRTRDRLSAT
>Mature_213_residues
PEPTCIAGKGAAIRFENLDTDQIIPKQFLRGIDKSGLAEGILYDHRFDASGAPRPGFVLNRPEYAGASILVSGANFGCGS
SREHAVWGLQQFGIRAVIAPSFGEIFYSNAMNNRLLLVMLPREVIGPLMNDVDADAANTISIDVEAMRVRTPSGDYAFEL
SARHRRMFIEGLDMIGLSLRQRGEIEQFIQQYRSAFPWTTNVARRTRDRLSAT

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=174, Percent_Identity=45.4022988505747, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=37.7551020408163, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23738; Mature: 23607

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPTCIAGKGAAIRFENLDTDQIIPKQFLRGIDKSGLAEGILYDHRFDASGAPRPGFVL
CCCCCEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHEECCCCCCCCCCCCCEEE
NRPEYAGASILVSGANFGCGSSREHAVWGLQQFGIRAVIAPSFGEIFYSNAMNNRLLLVM
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHCCCCCEEEEEE
LPREVIGPLMNDVDADAANTISIDVEAMRVRTPSGDYAFELSARHRRMFIEGLDMIGLSL
CCHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHH
RQRGEIEQFIQQYRSAFPWTTNVARRTRDRLSAT
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEPTCIAGKGAAIRFENLDTDQIIPKQFLRGIDKSGLAEGILYDHRFDASGAPRPGFVL
CCCCEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHEECCCCCCCCCCCCCEEE
NRPEYAGASILVSGANFGCGSSREHAVWGLQQFGIRAVIAPSFGEIFYSNAMNNRLLLVM
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHCCCCCEEEEEE
LPREVIGPLMNDVDADAANTISIDVEAMRVRTPSGDYAFELSARHRRMFIEGLDMIGLSL
CCHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHH
RQRGEIEQFIQQYRSAFPWTTNVARRTRDRLSAT
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]