Click here to switch to the map view.

The map label for this gene is leuC5 [H]

Identifier: 116696211

GI number: 116696211

Start: 2574507

End: 2575925

Strand: Direct

Name: leuC5 [H]

Synonym: H16_B2275

Alternate gene names: 116696211

Gene position: 2574507-2575925 (Clockwise)

Preceding gene: 116696210

Following gene: 116696212

Centisome position: 88.4

GC content: 68.71

Gene sequence:

>1419_bases
ATGACCCCCAAGACGCTATACCAGAAGCTGGTCGACTCCCATACCGTGGCCAGGCTCGACGACGACAACGTGCTGTTGTA
CTGCGACCTGCACCTGATGAACGAGTACACCAGCCCGCAGGCCTTCGCCGGGCTGCATGAACAGGACCGCGGCGTGCTGG
TGCCCGGACAGAACGTCTCGGTGGTCAGCCATATCATCCCGACGCACCCCACCGCGATCCGCGTGATCGCGGATCCGGCC
TCGGCGCTGCAGGCCACCAACCTGCGTGCCAACTGCGAGCGGCACGCGATCCCGCTGTTCGACACCAACGACACGCTGCA
GGGCATCGAGCATGTGATCGCGCCCGAGCACGGCATGATCCGGCCGGGCATGGTGGTGATCTGCGGCGACAGCCATACCA
CCACCTACGGTGCGCTCGGCGCGCTCGGCTTCGGCATCGGCACCTCCGAGGTCGAGCATGTGCTGGCCACCCAGACGCTG
GTCTACCGGATGGCCAGGACCATGCGCATCCGCGTGGACGGCAAGCTGCCCGCCGGGACCACCGCCAAGGACCTGATCCT
CAGGATCATCGGCGCGATCGGCGCGCAAGGTGCACGCGGCTATGTGGTGGAGTACTGCGGATCGGCGATCCGCGACCTGA
GCATCGAGGCCCGCTTCACGCTGTGCAATATGACTGTGGAGGCCGCGGCGCGCGGCGCGCTGATCGCGCCGGACGCGGTC
GCCACGGACTATGTGCTGCGCCGCGCCGTGGATATCGAAGGCCCGCAGCGCGACGCCGCGCTCAGCTACTGGGCGACTCT
CCAAAGCGATGCGGACGCCGTGTTCGACATGGACTTCGTGTTCGACGCAAGCGAGATCGAGCCCTACGTCACCTGGGGCA
CCAGCCCGGACCAGGTACTGCCGGTTTCCGGCCAGATCCCGTTCCCGGAAGACCAGCTCGACGAGGCCGAGCAGCGCTCG
GTCGAGCGCGCGCTGGCGTACACGCACCTGCAACCGGGCGCCGCGCTGGCAGGCACGCCGATCCAGCACGTCTTCATCGG
CTCCTGCACCAACGGCCGCATCGAGGACCTGCGCGCGGTGGCCAGCGTGGTGCGCGGCCGCAAGGTGGCCGCGGGGGTCA
GAGCCATGGTGGTGCCCGGGTCGGGCGCGGTCAAGGCCCAGGCCGAGCGCGAGGGCCTGGCCGAAGTCCTCACCGCCGCC
GGCTTTGAATGGCGACAACCGGGCTGCTCGATGTGCCTGGCCATGAACGACGACGTGCTCGCCGACGGCGTGCGCTGCGC
TTCCACCACCAACCGCAATTTCGAGGGCCGGCAGGGGCGCGGTGCCATCACCCACCTGATGAGCCCGGCCATGGCCGCTG
CGGCCGCCGTCACCGGCGTCATCACCGACGTCCGCAAGCTGGAGATCACCCATGCCTGA

Upstream 100 bases:

>100_bases
AGGCCGCGCGCCAGCGCCTGGAAAAGGAGATGCCCGTGTGGGCCAACCTGATCAGGCAGCGCGGCATCACCGCGGAATAA
CGACAGACAAGGCAGTTGCC

Downstream 100 bases:

>100_bases
ACCCACCTGCATCGCCGGCAAAGGCGCCGCCATCCGCTTCGAGAACCTCGATACCGACCAGATCATCCCCAAGCAGTTCC
TGCGCGGGATCGACAAGTCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTPKTLYQKLVDSHTVARLDDDNVLLYCDLHLMNEYTSPQAFAGLHEQDRGVLVPGQNVSVVSHIIPTHPTAIRVIADPA
SALQATNLRANCERHAIPLFDTNDTLQGIEHVIAPEHGMIRPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTL
VYRMARTMRIRVDGKLPAGTTAKDLILRIIGAIGAQGARGYVVEYCGSAIRDLSIEARFTLCNMTVEAAARGALIAPDAV
ATDYVLRRAVDIEGPQRDAALSYWATLQSDADAVFDMDFVFDASEIEPYVTWGTSPDQVLPVSGQIPFPEDQLDEAEQRS
VERALAYTHLQPGAALAGTPIQHVFIGSCTNGRIEDLRAVASVVRGRKVAAGVRAMVVPGSGAVKAQAEREGLAEVLTAA
GFEWRQPGCSMCLAMNDDVLADGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGVITDVRKLEITHA

Sequences:

>Translated_472_residues
MTPKTLYQKLVDSHTVARLDDDNVLLYCDLHLMNEYTSPQAFAGLHEQDRGVLVPGQNVSVVSHIIPTHPTAIRVIADPA
SALQATNLRANCERHAIPLFDTNDTLQGIEHVIAPEHGMIRPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTL
VYRMARTMRIRVDGKLPAGTTAKDLILRIIGAIGAQGARGYVVEYCGSAIRDLSIEARFTLCNMTVEAAARGALIAPDAV
ATDYVLRRAVDIEGPQRDAALSYWATLQSDADAVFDMDFVFDASEIEPYVTWGTSPDQVLPVSGQIPFPEDQLDEAEQRS
VERALAYTHLQPGAALAGTPIQHVFIGSCTNGRIEDLRAVASVVRGRKVAAGVRAMVVPGSGAVKAQAEREGLAEVLTAA
GFEWRQPGCSMCLAMNDDVLADGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGVITDVRKLEITHA
>Mature_471_residues
TPKTLYQKLVDSHTVARLDDDNVLLYCDLHLMNEYTSPQAFAGLHEQDRGVLVPGQNVSVVSHIIPTHPTAIRVIADPAS
ALQATNLRANCERHAIPLFDTNDTLQGIEHVIAPEHGMIRPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTLV
YRMARTMRIRVDGKLPAGTTAKDLILRIIGAIGAQGARGYVVEYCGSAIRDLSIEARFTLCNMTVEAAARGALIAPDAVA
TDYVLRRAVDIEGPQRDAALSYWATLQSDADAVFDMDFVFDASEIEPYVTWGTSPDQVLPVSGQIPFPEDQLDEAEQRSV
ERALAYTHLQPGAALAGTPIQHVFIGSCTNGRIEDLRAVASVVRGRKVAAGVRAMVVPGSGAVKAQAEREGLAEVLTAAG
FEWRQPGCSMCLAMNDDVLADGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGVITDVRKLEITHA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=26.7029972752044, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI8659555, Length=392, Percent_Identity=26.2755102040816, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=55.2688172043011, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=25.7703081232493, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367097, Length=357, Percent_Identity=26.3305322128852, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787531, Length=358, Percent_Identity=27.0949720670391, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=366, Percent_Identity=27.5956284153005, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564738, Length=366, Percent_Identity=27.5956284153005, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=25.9375, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17568399, Length=384, Percent_Identity=25.2604166666667, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=50, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322261, Length=363, Percent_Identity=27.5482093663912, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=392, Percent_Identity=28.3163265306122, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=28.7292817679558, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=26.1780104712042, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=26.1780104712042, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.1780104712042, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=28.1767955801105, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=25.2631578947368, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17137564, Length=416, Percent_Identity=24.0384615384615, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50476; Mature: 50345

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKTLYQKLVDSHTVARLDDDNVLLYCDLHLMNEYTSPQAFAGLHEQDRGVLVPGQNVS
CCHHHHHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCHHHHCCCCCCCEEEECCCCHH
VVSHIIPTHPTAIRVIADPASALQATNLRANCERHAIPLFDTNDTLQGIEHVIAPEHGMI
HHHHHHCCCCCEEEEEECCHHHHHHCCCCCCCHHCCCCEECCCHHHHHHHHHHCCCCCCC
RPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRMARTMRIRVDGKLPAGT
CCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
TAKDLILRIIGAIGAQGARGYVVEYCGSAIRDLSIEARFTLCNMTVEAAARGALIAPDAV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEEEEEEEHHHHHCCCEECCCHH
ATDYVLRRAVDIEGPQRDAALSYWATLQSDADAVFDMDFVFDASEIEPYVTWGTSPDQVL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHHEEEHHHCCCEEEECCCCCCEE
PVSGQIPFPEDQLDEAEQRSVERALAYTHLQPGAALAGTPIQHVFIGSCTNGRIEDLRAV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
ASVVRGRKVAAGVRAMVVPGSGAVKAQAEREGLAEVLTAAGFEWRQPGCSMCLAMNDDVL
HHHHCCCHHHCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHH
ADGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGVITDVRKLEITHA
HCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TPKTLYQKLVDSHTVARLDDDNVLLYCDLHLMNEYTSPQAFAGLHEQDRGVLVPGQNVS
CHHHHHHHHHCCCCEEEECCCCEEEEEEEEECCCCCCCHHHHCCCCCCCEEEECCCCHH
VVSHIIPTHPTAIRVIADPASALQATNLRANCERHAIPLFDTNDTLQGIEHVIAPEHGMI
HHHHHHCCCCCEEEEEECCHHHHHHCCCCCCCHHCCCCEECCCHHHHHHHHHHCCCCCCC
RPGMVVICGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRMARTMRIRVDGKLPAGT
CCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
TAKDLILRIIGAIGAQGARGYVVEYCGSAIRDLSIEARFTLCNMTVEAAARGALIAPDAV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEEEEEEEEHHHHHCCCEECCCHH
ATDYVLRRAVDIEGPQRDAALSYWATLQSDADAVFDMDFVFDASEIEPYVTWGTSPDQVL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHHEEEHHHCCCEEEECCCCCCEE
PVSGQIPFPEDQLDEAEQRSVERALAYTHLQPGAALAGTPIQHVFIGSCTNGRIEDLRAV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
ASVVRGRKVAAGVRAMVVPGSGAVKAQAEREGLAEVLTAAGFEWRQPGCSMCLAMNDDVL
HHHHCCCHHHCCCEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHH
ADGVRCASTTNRNFEGRQGRGAITHLMSPAMAAAAAVTGVITDVRKLEITHA
HCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA