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The map label for this gene is purU [H]

Identifier: 116695892

GI number: 116695892

Start: 2208640

End: 2209560

Strand: Direct

Name: purU [H]

Synonym: H16_B1956

Alternate gene names: 116695892

Gene position: 2208640-2209560 (Clockwise)

Preceding gene: 116695891

Following gene: 116695894

Centisome position: 75.83

GC content: 65.8

Gene sequence:

>921_bases
ATGCAAGCGGATACCGGAGTGCCCATGCCAGTGATCCAGACCGAAACCGCCAGCTACATCCTGACGCTGTCATGCCCCGA
CCGTCCCGGCATTGTCCATGCCGTTTCAGCGCTGCTGGTGGAAGCCGGCGCCAATATCGTCGATTCCGCGCAGTTCGGCG
ATCGCGACAGCGCACGGTTTTTCATGCGCGTGCACTTCCAGCGGCCGCTGCCCGAAGGCCAGGATGCCGGCGATGCGCTC
GCTGCCCTGCGCAGCACGCTGGCAGGCGTGGCGGCCGCGCATGAGATGGACTGGTCCCTCCACGACGCCGCGCGCAAGCC
GCGCGTGATGCTGATGGTGTCAAGGATCGGCCACTGCCTGAACGACCTGCTGTTCCGCTACCGCACCGGCCAGCTGCCCA
TCGAGATTCCGGCCATCGTCTCCAACCACAAGGACTTCTACCAGCTTGCGGCGAGCTACGACATCCCGTTCCATCACCTG
CCCCTGCTCAATGCCACGCCGCAAGGCAAGGCCGCGCAAGAAGCGCGGCTGTGGGACCTGGTCTGCGACTACAGCATCGA
CCTGGTCGTCCTGGCCCGTTACATGCAGGTCTTGTCCGACGACCTGTGCCGCCGCCTGGAGGGCCGCGCCATCAACATCC
ATCATTCCTTCCTGCCCAGCTTCAAGGGCGCGCGCCCGTACGCGCAAGCCTATGAGCGCGGCGTCAAGCTGATCGGCGCC
ACAGCGCACTACGTGACCGGCGACCTCGACGAAGGCCCCATCATCGAACAGGAAGTCGCGCGCGTGGACCATGCCATGGA
CGCCGCACAGCTGACCGCCATCGGACGCGACGTGGAATGCGTGGCGCTGGCGCGTGCGGTGAAATGGCATGCGGAACATC
GGGTCCTGCGCAACGGCGGCAGGACCCTGGTGTTCCGCTGA

Upstream 100 bases:

>100_bases
CAAGTATCACGTAGACCTGGCCGGGGAACTGCTGCCCGCGCGCATCCACCTGCGCGCGCCGTACGATCCGGCCAGCGAGC
GGACCAAGGCCTGAACCTCG

Downstream 100 bases:

>100_bases
CGCGGCAGCCTTTGCCGCAGCTTCAGCCGGCCACCGCCCGCCGGACCTTGCGCAACGCCTGCTCCAGCGCATCCATGGAA
ATCGACCCGAGCGCCAGCCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQADTGVPMPVIQTETASYILTLSCPDRPGIVHAVSALLVEAGANIVDSAQFGDRDSARFFMRVHFQRPLPEGQDAGDAL
AALRSTLAGVAAAHEMDWSLHDAARKPRVMLMVSRIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHL
PLLNATPQGKAAQEARLWDLVCDYSIDLVVLARYMQVLSDDLCRRLEGRAINIHHSFLPSFKGARPYAQAYERGVKLIGA
TAHYVTGDLDEGPIIEQEVARVDHAMDAAQLTAIGRDVECVALARAVKWHAEHRVLRNGGRTLVFR

Sequences:

>Translated_306_residues
MQADTGVPMPVIQTETASYILTLSCPDRPGIVHAVSALLVEAGANIVDSAQFGDRDSARFFMRVHFQRPLPEGQDAGDAL
AALRSTLAGVAAAHEMDWSLHDAARKPRVMLMVSRIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHL
PLLNATPQGKAAQEARLWDLVCDYSIDLVVLARYMQVLSDDLCRRLEGRAINIHHSFLPSFKGARPYAQAYERGVKLIGA
TAHYVTGDLDEGPIIEQEVARVDHAMDAAQLTAIGRDVECVALARAVKWHAEHRVLRNGGRTLVFR
>Mature_306_residues
MQADTGVPMPVIQTETASYILTLSCPDRPGIVHAVSALLVEAGANIVDSAQFGDRDSARFFMRVHFQRPLPEGQDAGDAL
AALRSTLAGVAAAHEMDWSLHDAARKPRVMLMVSRIGHCLNDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHL
PLLNATPQGKAAQEARLWDLVCDYSIDLVVLARYMQVLSDDLCRRLEGRAINIHHSFLPSFKGARPYAQAYERGVKLIGA
TAHYVTGDLDEGPIIEQEVARVDHAMDAAQLTAIGRDVECVALARAVKWHAEHRVLRNGGRTLVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=171, Percent_Identity=33.9181286549708, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI209869995, Length=171, Percent_Identity=33.9181286549708, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI209869993, Length=171, Percent_Identity=33.9181286549708, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787483, Length=286, Percent_Identity=39.5104895104895, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=27.1276595744681, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=161, Percent_Identity=32.2981366459627, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582400, Length=176, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33834; Mature: 33834

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQADTGVPMPVIQTETASYILTLSCPDRPGIVHAVSALLVEAGANIVDSAQFGDRDSARF
CCCCCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEE
FMRVHFQRPLPEGQDAGDALAALRSTLAGVAAAHEMDWSLHDAARKPRVMLMVSRIGHCL
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHH
NDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHLPLLNATPQGKAAQEARLWDL
HHHHHHHCCCCCCEEEHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHH
VCDYSIDLVVLARYMQVLSDDLCRRLEGRAINIHHSFLPSFKGARPYAQAYERGVKLIGA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCHHEEEC
TAHYVTGDLDEGPIIEQEVARVDHAMDAAQLTAIGRDVECVALARAVKWHAEHRVLRNGG
CHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
RTLVFR
CEEEEC
>Mature Secondary Structure
MQADTGVPMPVIQTETASYILTLSCPDRPGIVHAVSALLVEAGANIVDSAQFGDRDSARF
CCCCCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEE
FMRVHFQRPLPEGQDAGDALAALRSTLAGVAAAHEMDWSLHDAARKPRVMLMVSRIGHCL
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHH
NDLLFRYRTGQLPIEIPAIVSNHKDFYQLAASYDIPFHHLPLLNATPQGKAAQEARLWDL
HHHHHHHCCCCCCEEEHHHHCCCHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHH
VCDYSIDLVVLARYMQVLSDDLCRRLEGRAINIHHSFLPSFKGARPYAQAYERGVKLIGA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCHHEEEC
TAHYVTGDLDEGPIIEQEVARVDHAMDAAQLTAIGRDVECVALARAVKWHAEHRVLRNGG
CHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
RTLVFR
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]