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The map label for this gene is shiA [H]

Identifier: 116695788

GI number: 116695788

Start: 2092292

End: 2093620

Strand: Direct

Name: shiA [H]

Synonym: H16_B1852

Alternate gene names: 116695788

Gene position: 2092292-2093620 (Clockwise)

Preceding gene: 116695787

Following gene: 116695789

Centisome position: 71.84

GC content: 63.58

Gene sequence:

>1329_bases
ATGACAAGCCCCAACGAACAGGCGATCCGCAAGGTCGCGCTGGCGTCCGTGATCGGCGCCACCATCGAATGGTATGACTT
CTTCCTGTACGGCGTGGTCGCCGGCCTGGTCTTCAACAAGCTGTACTTCCCCGGCAATGACCCGCTGGTGGCCACCATGC
TGGCCTACACCACCTTTGCGGTGGGCTTTGTCACGCGGCCGCTGGGCGGCGTGATCTTCGGGCACTTTGGCGACAAGGTT
GGCCGCAAGAGCATGCTGGTGATCACGCTGATGATCATGGGCGTGTCCACCTTCCTGATCGGGCTGGTGCCGACCTATGA
CAGCATCGGCATCCTGGCGCCGATCCTGTTGCTGCTGCTGCGCGTGCTGCAGGGCATCGGCCTGGGTGGCGAATGGGGCG
GCGCGGTGCTGATGGCCTATGAGTACGCACCGCCGGGCAAACGGGGCTTCTATGCGTCGCTGCCGCAGATCGGGCTGGCG
ATCGGACTGTGCCTGGCATCCGGCGTGGTGGCGCTGCTGTCGCTGACCCTGACCGATACGCAGTTTCTGGCGTGGGGCTG
GCGCGTGGCCTTCCTGATCTCGGCGGGGATGGTGTTCGTGGGCATGTATATCCGCCTCAACGTCAAGGAGACACCTGAAT
TCGCCGTGATCAAGCAGCGCAATGCCGAGACCCAGATTCCCTTTGTCGACATGATGCGGCGCTACCCGGGCAATATCCTC
AAGGGCATGGGCGCACGCTATATCGACGGGGTCTTCTTCAACATCTTCGGCGTGTTCTCGATCACCTACCTGACCCAGAC
CATCAATATCAGCCGGACTGAAGCGCTGGTCGGCGTAATGGCGGCGGCCATTGCGATGTGCTTCTTCATTCCGTTCTTCG
GCCACCTGTCCGACCGCATCGGCCGCACCCGCGTGTATTTCTGGGGCTCGCTGATCACGGCGCTGTCCGCCTTCCCGGCG
TTCTGGCTGATGCTGAACAGCGGCGGCAACACCATGCTGCTGTGGCTGTCCATCGTGGTGCCGTTCGGCATCCTCTACGC
CGCGGTCTATGGCCCGGAAGCTGCGCTGTTCTGCGAGCTGTTCGATGCCAGGGTGCGATACACCGGGATCTCGTTCGTCT
ACCAGTTCTCGGGAATCTTCGCCTCCGGCATCACGCCCATCATCGCCACGGCACTGCTCAAGTCGGGCGGCGGCAAGCCC
TGGCAGATCTGCGCCTACGTTGCCTTTGCGGGGGTGGTGTCGGCGTTGTCGGTGGCGCTGATCGGACGGGGCGAAGGCTC
CCAGGCGCCGCGTGAAAACGGCATGCCGCTGCGCGGTCCGGCGCGATAG

Upstream 100 bases:

>100_bases
ACCAAGGCCAGGCACCCATAACAAGGCCAAACCCTGCAGTGCATCCGGCGTCCATAACCATCCTTCCAGAGGACGCCGCC
CATCACCAGAGGAGACAAGC

Downstream 100 bases:

>100_bases
GCGAGGAGCGGGCCGCCGCCCGCCGGCAATGTCTTAGAATGGCGCAACGCCAATGACAGGGACATTGCATGCAAACTCCG
GACGGCACCGGTGCAGCCGC

Product: major facilitator superfamily transporter MHS family protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSPNEQAIRKVALASVIGATIEWYDFFLYGVVAGLVFNKLYFPGNDPLVATMLAYTTFAVGFVTRPLGGVIFGHFGDKV
GRKSMLVITLMIMGVSTFLIGLVPTYDSIGILAPILLLLLRVLQGIGLGGEWGGAVLMAYEYAPPGKRGFYASLPQIGLA
IGLCLASGVVALLSLTLTDTQFLAWGWRVAFLISAGMVFVGMYIRLNVKETPEFAVIKQRNAETQIPFVDMMRRYPGNIL
KGMGARYIDGVFFNIFGVFSITYLTQTINISRTEALVGVMAAAIAMCFFIPFFGHLSDRIGRTRVYFWGSLITALSAFPA
FWLMLNSGGNTMLLWLSIVVPFGILYAAVYGPEAALFCELFDARVRYTGISFVYQFSGIFASGITPIIATALLKSGGGKP
WQICAYVAFAGVVSALSVALIGRGEGSQAPRENGMPLRGPAR

Sequences:

>Translated_442_residues
MTSPNEQAIRKVALASVIGATIEWYDFFLYGVVAGLVFNKLYFPGNDPLVATMLAYTTFAVGFVTRPLGGVIFGHFGDKV
GRKSMLVITLMIMGVSTFLIGLVPTYDSIGILAPILLLLLRVLQGIGLGGEWGGAVLMAYEYAPPGKRGFYASLPQIGLA
IGLCLASGVVALLSLTLTDTQFLAWGWRVAFLISAGMVFVGMYIRLNVKETPEFAVIKQRNAETQIPFVDMMRRYPGNIL
KGMGARYIDGVFFNIFGVFSITYLTQTINISRTEALVGVMAAAIAMCFFIPFFGHLSDRIGRTRVYFWGSLITALSAFPA
FWLMLNSGGNTMLLWLSIVVPFGILYAAVYGPEAALFCELFDARVRYTGISFVYQFSGIFASGITPIIATALLKSGGGKP
WQICAYVAFAGVVSALSVALIGRGEGSQAPRENGMPLRGPAR
>Mature_441_residues
TSPNEQAIRKVALASVIGATIEWYDFFLYGVVAGLVFNKLYFPGNDPLVATMLAYTTFAVGFVTRPLGGVIFGHFGDKVG
RKSMLVITLMIMGVSTFLIGLVPTYDSIGILAPILLLLLRVLQGIGLGGEWGGAVLMAYEYAPPGKRGFYASLPQIGLAI
GLCLASGVVALLSLTLTDTQFLAWGWRVAFLISAGMVFVGMYIRLNVKETPEFAVIKQRNAETQIPFVDMMRRYPGNILK
GMGARYIDGVFFNIFGVFSITYLTQTINISRTEALVGVMAAAIAMCFFIPFFGHLSDRIGRTRVYFWGSLITALSAFPAF
WLMLNSGGNTMLLWLSIVVPFGILYAAVYGPEAALFCELFDARVRYTGISFVYQFSGIFASGITPIIATALLKSGGGKPW
QICAYVAFAGVVSALSVALIGRGEGSQAPRENGMPLRGPAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=40.2777777777778, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789941, Length=392, Percent_Identity=41.3265306122449, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1790550, Length=443, Percent_Identity=33.4085778781038, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=30.622009569378, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=23.4848484848485, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082231, Length=173, Percent_Identity=24.2774566473988, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47805; Mature: 47674

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPNEQAIRKVALASVIGATIEWYDFFLYGVVAGLVFNKLYFPGNDPLVATMLAYTTFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VGFVTRPLGGVIFGHFGDKVGRKSMLVITLMIMGVSTFLIGLVPTYDSIGILAPILLLLL
HHHHHCCHHHHEEEHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RVLQGIGLGGEWGGAVLMAYEYAPPGKRGFYASLPQIGLAIGLCLASGVVALLSLTLTDT
HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
QFLAWGWRVAFLISAGMVFVGMYIRLNVKETPEFAVIKQRNAETQIPFVDMMRRYPGNIL
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCHHH
KGMGARYIDGVFFNIFGVFSITYLTQTINISRTEALVGVMAAAIAMCFFIPFFGHLSDRI
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRTRVYFWGSLITALSAFPAFWLMLNSGGNTMLLWLSIVVPFGILYAAVYGPEAALFCEL
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FDARVRYTGISFVYQFSGIFASGITPIIATALLKSGGGKPWQICAYVAFAGVVSALSVAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGRGEGSQAPRENGMPLRGPAR
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSPNEQAIRKVALASVIGATIEWYDFFLYGVVAGLVFNKLYFPGNDPLVATMLAYTTFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VGFVTRPLGGVIFGHFGDKVGRKSMLVITLMIMGVSTFLIGLVPTYDSIGILAPILLLLL
HHHHHCCHHHHEEEHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RVLQGIGLGGEWGGAVLMAYEYAPPGKRGFYASLPQIGLAIGLCLASGVVALLSLTLTDT
HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
QFLAWGWRVAFLISAGMVFVGMYIRLNVKETPEFAVIKQRNAETQIPFVDMMRRYPGNIL
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCHHH
KGMGARYIDGVFFNIFGVFSITYLTQTINISRTEALVGVMAAAIAMCFFIPFFGHLSDRI
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRTRVYFWGSLITALSAFPAFWLMLNSGGNTMLLWLSIVVPFGILYAAVYGPEAALFCEL
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FDARVRYTGISFVYQFSGIFASGITPIIATALLKSGGGKPWQICAYVAFAGVVSALSVAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGRGEGSQAPRENGMPLRGPAR
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]