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The map label for this gene is alkJ [H]

Identifier: 116695787

GI number: 116695787

Start: 2090507

End: 2092162

Strand: Direct

Name: alkJ [H]

Synonym: H16_B1851

Alternate gene names: 116695787

Gene position: 2090507-2092162 (Clockwise)

Preceding gene: 116695785

Following gene: 116695788

Centisome position: 71.78

GC content: 68.48

Gene sequence:

>1656_bases
ATGTCGCAAACCGTTGACTACATCGTGGTAGGCGCCGGCTCGGCCGGGTGCGTGCTGGCCAACCGGCTCAGCGAAGACGG
CCGCTATTCGGTCTGCCTGCTGGAGGCCGGCCCGCCCGACCGCTACCCGTGGATCCATATCCCGATCGGCTATGGCAAGA
CCATGTTCCACAAGCAAGTGAACTGGGGCTTCTATACGGATCCCGATCCCAACATGCTCGATCGCCGCATCTACTGGCCG
CGCGGCCGCACGCTGGGCGGCAGCAGCGCCATCAACGGCCTGATCTACGTACGCGGCCAGCGCGAGGACTATGACCACTG
GGAAACGCTGGGCAATCCGGGCTGGAGCTGGGACAACTGCCTGCCCTACTTCCGCAAGCTCGAGAACAACGACCTTGGCG
CCGGCCCCACGCGCGGCACCGACGGCCCGCTGAACGCGACCTCGATCGACCGGCAGCATCCGCTGGTCGATGCCTTCATC
GGCGCCGGCCAGGCGCTGGGCCTGCCCCGCAAGACCGACTTCAACGGTGGCGACCAGGAGGGCGTGGGCTATTACCAGCT
CACCACCCGCAACGGCTGGCGCTGCTCCACCGCGGTGGCCTACCTGCGCCCGGCGCGCGGGCGCACGAACCTGCGCGTCG
AGACCGATGCCCACACCACCGGGATCCTGTTCGAAGGCAAGCGTGCCGTGGGCGTGCGCTACACCCAGCATGGCCAGCCC
TATATCCTGCGGGCGCGGCGCGAGGTGATCCTGTGCGCGGGCGCGCTGCAGTCGCCGCAGTTGCTGCAGCTGTCGGGCAT
CGGCCCGGCGCCGTTGCTGCAGGACCTGGGCGTACCGGTGGTGCATGCCCTGCCGGGCGTGGGCGAGAACCTGCAGGACC
ATCTGCAGGTGCGACTGATCTACGAGGTGGCCAAGCCGATCACCACCAACGACCAGCTGCGCTCGCTGACGGGCAAGGCG
CGCATGGGCCTGGAATGGCTGCTGATGCGCAAGGGGCCGCTGGCGATCGGCATCAACCAGGGCGCGATGTTCTGCCGCGC
ACTGCCGCAGGAAAGCGCCACGCCGGACACGCAGTTCCATTTCTCGACCCTGTCCGCCGACATGGCGGGCGGCACGGTGC
ATCCCTTCTCCGGCTGCACCTATTCGGTATGCCAGTTGCGGCCCGAGTCGCGCGGCACGGTGCGGATCCGCTCGACCGAT
CCGTATGAGCCGCCGTCGATGCAGCCCAACTACCTGTCGGCCGAGCTGGACCGGCGCTGCACCGTGGCAGCGGTGCGCTA
CGCCCGGCGCGTGGCGCAGGCCGAACCGATGCGCGGCCTGATGAAGCGCGAGTTCCGGCCGGGCGACGAGGTGCGCAGCG
ACGACGAGATCCTGCATTTCTGCCGCGAGTACGGTGCCACCATCTTCCATCCGTCGGGCACCGCGAAAATGGGGCCCGCC
GCCGATCCATTGGCAGTGGTCGATGCGCGCCTGCGCGTGCATGGCGTGGGCGGGCTGCGCGTGGTGGACTGCTCGGTGAT
GCCTACGCTGGTCTCAGGCAACACCAACGTGCCGGTGGTGATGATGGCCGAGCGCGCGGCCGACTTCATCCGCGAAGACG
CACGGCGGGAAGTGCATCCCGTCGACGTGGCAACCAAGATGGCAGCAGCCGCCTGA

Upstream 100 bases:

>100_bases
ATTCCGGCTTTGAAAAATAAAATCCGGAACAGAATGTTTCAGATTATAGATCCAAGCCGGATGGTTACGTGCCTGCCGGC
CAAAAAAGGAGACAGCGACG

Downstream 100 bases:

>100_bases
GCGGCCTGCGCCAATCCTGATTGCGAACAACCAAGGCCAGGCACCCATAACAAGGCCAAACCCTGCAGTGCATCCGGCGT
CCATAACCATCCTTCCAGAG

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MSQTVDYIVVGAGSAGCVLANRLSEDGRYSVCLLEAGPPDRYPWIHIPIGYGKTMFHKQVNWGFYTDPDPNMLDRRIYWP
RGRTLGGSSAINGLIYVRGQREDYDHWETLGNPGWSWDNCLPYFRKLENNDLGAGPTRGTDGPLNATSIDRQHPLVDAFI
GAGQALGLPRKTDFNGGDQEGVGYYQLTTRNGWRCSTAVAYLRPARGRTNLRVETDAHTTGILFEGKRAVGVRYTQHGQP
YILRARREVILCAGALQSPQLLQLSGIGPAPLLQDLGVPVVHALPGVGENLQDHLQVRLIYEVAKPITTNDQLRSLTGKA
RMGLEWLLMRKGPLAIGINQGAMFCRALPQESATPDTQFHFSTLSADMAGGTVHPFSGCTYSVCQLRPESRGTVRIRSTD
PYEPPSMQPNYLSAELDRRCTVAAVRYARRVAQAEPMRGLMKREFRPGDEVRSDDEILHFCREYGATIFHPSGTAKMGPA
ADPLAVVDARLRVHGVGGLRVVDCSVMPTLVSGNTNVPVVMMAERAADFIREDARREVHPVDVATKMAAAA

Sequences:

>Translated_551_residues
MSQTVDYIVVGAGSAGCVLANRLSEDGRYSVCLLEAGPPDRYPWIHIPIGYGKTMFHKQVNWGFYTDPDPNMLDRRIYWP
RGRTLGGSSAINGLIYVRGQREDYDHWETLGNPGWSWDNCLPYFRKLENNDLGAGPTRGTDGPLNATSIDRQHPLVDAFI
GAGQALGLPRKTDFNGGDQEGVGYYQLTTRNGWRCSTAVAYLRPARGRTNLRVETDAHTTGILFEGKRAVGVRYTQHGQP
YILRARREVILCAGALQSPQLLQLSGIGPAPLLQDLGVPVVHALPGVGENLQDHLQVRLIYEVAKPITTNDQLRSLTGKA
RMGLEWLLMRKGPLAIGINQGAMFCRALPQESATPDTQFHFSTLSADMAGGTVHPFSGCTYSVCQLRPESRGTVRIRSTD
PYEPPSMQPNYLSAELDRRCTVAAVRYARRVAQAEPMRGLMKREFRPGDEVRSDDEILHFCREYGATIFHPSGTAKMGPA
ADPLAVVDARLRVHGVGGLRVVDCSVMPTLVSGNTNVPVVMMAERAADFIREDARREVHPVDVATKMAAAA
>Mature_550_residues
SQTVDYIVVGAGSAGCVLANRLSEDGRYSVCLLEAGPPDRYPWIHIPIGYGKTMFHKQVNWGFYTDPDPNMLDRRIYWPR
GRTLGGSSAINGLIYVRGQREDYDHWETLGNPGWSWDNCLPYFRKLENNDLGAGPTRGTDGPLNATSIDRQHPLVDAFIG
AGQALGLPRKTDFNGGDQEGVGYYQLTTRNGWRCSTAVAYLRPARGRTNLRVETDAHTTGILFEGKRAVGVRYTQHGQPY
ILRARREVILCAGALQSPQLLQLSGIGPAPLLQDLGVPVVHALPGVGENLQDHLQVRLIYEVAKPITTNDQLRSLTGKAR
MGLEWLLMRKGPLAIGINQGAMFCRALPQESATPDTQFHFSTLSADMAGGTVHPFSGCTYSVCQLRPESRGTVRIRSTDP
YEPPSMQPNYLSAELDRRCTVAAVRYARRVAQAEPMRGLMKREFRPGDEVRSDDEILHFCREYGATIFHPSGTAKMGPAA
DPLAVVDARLRVHGVGGLRVVDCSVMPTLVSGNTNVPVVMMAERAADFIREDARREVHPVDVATKMAAAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=533, Percent_Identity=44.6529080675422, Blast_Score=431, Evalue=1e-121,
Organism=Escherichia coli, GI1786503, Length=534, Percent_Identity=43.0711610486891, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17532301, Length=549, Percent_Identity=39.7085610200364, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24642042, Length=567, Percent_Identity=41.0934744268078, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45549471, Length=567, Percent_Identity=38.8007054673721, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45551458, Length=567, Percent_Identity=38.8007054673721, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24642059, Length=566, Percent_Identity=38.1625441696113, Blast_Score=351, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24650267, Length=574, Percent_Identity=37.2822299651568, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24642055, Length=588, Percent_Identity=37.0748299319728, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24642048, Length=566, Percent_Identity=37.8091872791519, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI18859995, Length=576, Percent_Identity=37.6736111111111, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI17137792, Length=550, Percent_Identity=37.0909090909091, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24642039, Length=574, Percent_Identity=35.3658536585366, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24642051, Length=579, Percent_Identity=33.8514680483592, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24642037, Length=572, Percent_Identity=34.965034965035, Blast_Score=288, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24642035, Length=583, Percent_Identity=33.4476843910806, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859993, Length=570, Percent_Identity=33.1578947368421, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24642057, Length=577, Percent_Identity=31.0225303292894, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24645930, Length=577, Percent_Identity=29.9826689774697, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 60596; Mature: 60465

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVDYIVVGAGSAGCVLANRLSEDGRYSVCLLEAGPPDRYPWIHIPIGYGKTMFHKQV
CCCCEEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHEEC
NWGFYTDPDPNMLDRRIYWPRGRTLGGSSAINGLIYVRGQREDYDHWETLGNPGWSWDNC
CCCCCCCCCCCHHCCEEECCCCCCCCCCHHCCEEEEEECCCCCCHHHHHCCCCCCCHHHH
LPYFRKLENNDLGAGPTRGTDGPLNATSIDRQHPLVDAFIGAGQALGLPRKTDFNGGDQE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
GVGYYQLTTRNGWRCSTAVAYLRPARGRTNLRVETDAHTTGILFEGKRAVGVRYTQHGQP
CCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCEEEEEEEECCCCC
YILRARREVILCAGALQSPQLLQLSGIGPAPLLQDLGVPVVHALPGVGENLQDHLQVRLI
EEEECCCEEEEEECCCCCCCEEEECCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHEE
YEVAKPITTNDQLRSLTGKARMGLEWLLMRKGPLAIGINQGAMFCRALPQESATPDTQFH
EHHHCCCCCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCEEE
FSTLSADMAGGTVHPFSGCTYSVCQLRPESRGTVRIRSTDPYEPPSMQPNYLSAELDRRC
EEEHHHHCCCCEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCH
TVAAVRYARRVAQAEPMRGLMKREFRPGDEVRSDDEILHFCREYGATIFHPSGTAKMGPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCC
ADPLAVVDARLRVHGVGGLRVVDCSVMPTLVSGNTNVPVVMMAERAADFIREDARREVHP
CCCHHHEEEEEEEEECCCEEEEEEEECEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCC
VDVATKMAAAA
HHHHHHHHHCC
>Mature Secondary Structure 
SQTVDYIVVGAGSAGCVLANRLSEDGRYSVCLLEAGPPDRYPWIHIPIGYGKTMFHKQV
CCCEEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHEEC
NWGFYTDPDPNMLDRRIYWPRGRTLGGSSAINGLIYVRGQREDYDHWETLGNPGWSWDNC
CCCCCCCCCCCHHCCEEECCCCCCCCCCHHCCEEEEEECCCCCCHHHHHCCCCCCCHHHH
LPYFRKLENNDLGAGPTRGTDGPLNATSIDRQHPLVDAFIGAGQALGLPRKTDFNGGDQE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
GVGYYQLTTRNGWRCSTAVAYLRPARGRTNLRVETDAHTTGILFEGKRAVGVRYTQHGQP
CCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCEEEEEEEECCCCC
YILRARREVILCAGALQSPQLLQLSGIGPAPLLQDLGVPVVHALPGVGENLQDHLQVRLI
EEEECCCEEEEEECCCCCCCEEEECCCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHEE
YEVAKPITTNDQLRSLTGKARMGLEWLLMRKGPLAIGINQGAMFCRALPQESATPDTQFH
EHHHCCCCCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCEEE
FSTLSADMAGGTVHPFSGCTYSVCQLRPESRGTVRIRSTDPYEPPSMQPNYLSAELDRRC
EEEHHHHCCCCEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCH
TVAAVRYARRVAQAEPMRGLMKREFRPGDEVRSDDEILHFCREYGATIFHPSGTAKMGPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCC
ADPLAVVDARLRVHGVGGLRVVDCSVMPTLVSGNTNVPVVMMAERAADFIREDARREVHP
CCCHHHEEEEEEEEECCCEEEEEEEECEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCC
VDVATKMAAAA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]