Click here to switch to the map view.

The map label for this gene is est [H]

Identifier: 116695057

GI number: 116695057

Start: 1259588

End: 1260553

Strand: Direct

Name: est [H]

Synonym: H16_B1112

Alternate gene names: 116695057

Gene position: 1259588-1260553 (Clockwise)

Preceding gene: 116695054

Following gene: 116695058

Centisome position: 43.25

GC content: 72.36

Gene sequence:

>966_bases
ATGTCCGAGAAGCCAGGCCAACGCCCTGATGCAGCCAGCGCCGCCTCTGCCCCCACGGCAGCCGGGCAGGGGCAGGTGCG
CGTGGCCGAGCATACGGTGACCAGTGACGGGCGCGAGATCCTGGTTCGGGTGTGCTACCCGGCGGGCTATCCGGCGCCGG
GCGCCGAGGGCGCCGCGCTGCTGCCCTGGCTGGTGTACCTGCATGCCGGCGGCTTCGTCGAAGGCGGCCTGGAACACGCA
TGCCATCTGGTGCAGGACCTGGCCACCACGGTGCCGGCGGTGGTGGTAACGCCGGCGTATTCGCTGGCGCCGGACCATCC
GTTCCCGGCGGCGCCGGAGGACGCGCACAGCACGGTGCAGTGGGTGCTGCGCAATGCCCGGCGGCTGAAGCTGGACAAGA
TCCGCTTTGCGCTGGTGGGCGAGGAGGCCGGGGGCAACCTGGCGATCGCGCTGGCGCAGATGCTGCGCGACCGCGGCACC
GCGCAACCGCGCGGGCAATGGCTGATCCGCCCGGTGACGGACCCGTGCCTGCAGCATGCGTCCTGCGCGGGGTTCGACGG
CGGGCAGGTGCCGATGGACACCCTGCGGCGCCTGGCCGACAACTATCGCGATTACCTGCCGACGCCGGCGGATACGGTGC
ATCCGTATGCCGCCCCGGCGCTGGCCTCGCGCCTGGCCGGGCTGCCGCCCACGCTGGTCCAGGTGGCTGAATACGACGCG
CTGCGTGCCGAAGGCGAAGCCTTCGCCGGGCGCCTGGCCAAGGCCGGTGTGCCGGCCCAGGCCATGATCATGCCCGGCGC
CTGCGGCGACGGCGTGGAAGAGGCCCATGACAGCTGTCAGGCATGGGTCGACGAAGGTGCGCGGTTCCTGCGGCGATGTC
TGGCCGCGGCCGACGATACCTCATCCCTGACTGAACGCCGCGCTGACGCCAACGCCAGCCGGCCTGGAGCCAAGCCCGCC
GGCTGA

Upstream 100 bases:

>100_bases
AGTGCATTGGCGATCTCTCGCTTTATCCCATCCTCGTCAATCACCATAATCCGTTGCATCGCAACACTCAAGCGCATTGT
TTCCGCCGTGCGCCAACGCC

Downstream 100 bases:

>100_bases
GCCGCTCCAGACAACCGCCAGCCGGCTCTGTTTCGGCCAGAGCCAGGACCGCCGCGACACGCAAGCGGTCCTAAGTCCGA
CCATCAGTTTGGCCATGGTT

Product: esterase/ipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSEKPGQRPDAASAASAPTAAGQGQVRVAEHTVTSDGREILVRVCYPAGYPAPGAEGAALLPWLVYLHAGGFVEGGLEHA
CHLVQDLATTVPAVVVTPAYSLAPDHPFPAAPEDAHSTVQWVLRNARRLKLDKIRFALVGEEAGGNLAIALAQMLRDRGT
AQPRGQWLIRPVTDPCLQHASCAGFDGGQVPMDTLRRLADNYRDYLPTPADTVHPYAAPALASRLAGLPPTLVQVAEYDA
LRAEGEAFAGRLAKAGVPAQAMIMPGACGDGVEEAHDSCQAWVDEGARFLRRCLAAADDTSSLTERRADANASRPGAKPA
G

Sequences:

>Translated_321_residues
MSEKPGQRPDAASAASAPTAAGQGQVRVAEHTVTSDGREILVRVCYPAGYPAPGAEGAALLPWLVYLHAGGFVEGGLEHA
CHLVQDLATTVPAVVVTPAYSLAPDHPFPAAPEDAHSTVQWVLRNARRLKLDKIRFALVGEEAGGNLAIALAQMLRDRGT
AQPRGQWLIRPVTDPCLQHASCAGFDGGQVPMDTLRRLADNYRDYLPTPADTVHPYAAPALASRLAGLPPTLVQVAEYDA
LRAEGEAFAGRLAKAGVPAQAMIMPGACGDGVEEAHDSCQAWVDEGARFLRRCLAAADDTSSLTERRADANASRPGAKPA
G
>Mature_320_residues
SEKPGQRPDAASAASAPTAAGQGQVRVAEHTVTSDGREILVRVCYPAGYPAPGAEGAALLPWLVYLHAGGFVEGGLEHAC
HLVQDLATTVPAVVVTPAYSLAPDHPFPAAPEDAHSTVQWVLRNARRLKLDKIRFALVGEEAGGNLAIALAQMLRDRGTA
QPRGQWLIRPVTDPCLQHASCAGFDGGQVPMDTLRRLADNYRDYLPTPADTVHPYAAPALASRLAGLPPTLVQVAEYDAL
RAEGEAFAGRLAKAGVPAQAMIMPGACGDGVEEAHDSCQAWVDEGARFLRRCLAAADDTSSLTERRADANASRPGAKPAG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17567059, Length=252, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33715; Mature: 33584

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKPGQRPDAASAASAPTAAGQGQVRVAEHTVTSDGREILVRVCYPAGYPAPGAEGAAL
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCHHHEEHHHCCCCCCCCCCCCCHHH
LPWLVYLHAGGFVEGGLEHACHLVQDLATTVPAVVVTPAYSLAPDHPFPAAPEDAHSTVQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEECCCHHCCCCCCCCCCCCHHHHHHH
WVLRNARRLKLDKIRFALVGEEAGGNLAIALAQMLRDRGTAQPRGQWLIRPVTDPCLQHA
HHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHC
SCAGFDGGQVPMDTLRRLADNYRDYLPTPADTVHPYAAPALASRLAGLPPTLVQVAEYDA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LRAEGEAFAGRLAKAGVPAQAMIMPGACGDGVEEAHDSCQAWVDEGARFLRRCLAAADDT
HHHCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SSLTERRADANASRPGAKPAG
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKPGQRPDAASAASAPTAAGQGQVRVAEHTVTSDGREILVRVCYPAGYPAPGAEGAAL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCHHHEEHHHCCCCCCCCCCCCCHHH
LPWLVYLHAGGFVEGGLEHACHLVQDLATTVPAVVVTPAYSLAPDHPFPAAPEDAHSTVQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEECCCHHCCCCCCCCCCCCHHHHHHH
WVLRNARRLKLDKIRFALVGEEAGGNLAIALAQMLRDRGTAQPRGQWLIRPVTDPCLQHA
HHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHC
SCAGFDGGQVPMDTLRRLADNYRDYLPTPADTVHPYAAPALASRLAGLPPTLVQVAEYDA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LRAEGEAFAGRLAKAGVPAQAMIMPGACGDGVEEAHDSCQAWVDEGARFLRRCLAAADDT
HHHCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SSLTERRADANASRPGAKPAG
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]