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The map label for this gene is puuB [H]

Identifier: 116691161

GI number: 116691161

Start: 3460711

End: 3462099

Strand: Direct

Name: puuB [H]

Synonym: Bcen2424_3143

Alternate gene names: 116691161

Gene position: 3460711-3462099 (Clockwise)

Preceding gene: 116691155

Following gene: 116691164

Centisome position: 99.33

GC content: 70.05

Gene sequence:

>1389_bases
ATGCGACCCTTCTGGATCGAACAAGCGCTGTTCAACGACGGCGATCTCGCCCCCGCGCTGCAAGGCGCGACGCAGGCCGA
CGTGTGCATCGTCGGCGGCGGCTTCACGGGCCTCTGGACCGCGATCCAGGCGAAGCAGCACGACCCCGCGCTCGACATCG
CGATCCTCGAGGCCGACCTGTGCGGCGCGGGCGCGAGCGGCCGCAACGGCGGCTGCCTGCTCACGTGGTCCGCGAAATTC
CTGACGCTGCGGCGCCTGTTCGGCGAAGCCGAGGCGATCCGGCTCGTTAAGGCATCGGAAGCGGCCGTGCAGCACATCGC
CGATTTCTGCCGCGCGCACGACATCGACGCCGAATTGCGGCTCGACGGCACGCTGTACACCGCGACGTCGGGCGCGCAGG
TCGGCACGCTCGCGCCGGTGCTCGACGCGCTCGCCGCGTGCGGCATCCACAGCTACGAGCCGCTGCCGTCCGCCGAAGTC
GCGCGCCGCTCGGGCTCCGCGCGCAATCTCGACGGCGTCTACTCGCCGATCGCCGCGACCGTGCATCCCGGCAAGCTCGT
GCGCGGGCTGCGCCGCGTCGCGCTTGCGATGGGCATCCGCATCTACGAGCGCACGCCGATGCTCGACTTCACGCCCGGCC
AGCCGGCCGTCGTGCGTACGCCGTCCGGCAGCGTCACGGCGGGCAAGCTCGTGTTCGCGATCAATGCGTGGATGGCGAGC
CGCTTCCCGCAGTTCGAACGGACGATCGCGGTGGTGTCGAGCGACATGGTGATCACCGAGAAATGCCCCGAGCTGCTCGC
GCGGACCGGGCTCGTGGACGGCGTATCGGTGCTCGATTCGCGGATCTTCGTGTACTACTACCGCACGACCGCCGACGGGC
GGCTGATGCTCGGCAAAGGCGGCAACACGTTCTCGTGGCGCAGCCGCATCGCGCCCGTGTTCGACCGCCGCTCGCCGTAC
GAGGCGCAGCTCACGCGCAGCCTGCGCGAATTCTTCCCGTCGCTCGCCGGCGTGCCGATCACCGCGAGCTGGAACGGGCC
GTCGGATCGTTCGGTGACGGGTTTCCCGTTCTTCGGCCGCCTCGATGACGCGCCGAACGTGTTCTACGGCTTCGGCTATT
CGGGCAATGGCGTCGGGCCGACCTACATGGGCGGGCAGATCCTGTCGTCGCTGGTGCTCGGCCTCGACAACGCATGGACG
CGCAGCCCGATCGTGCGCGGCCCGCTCGGCCATTTCCCGCCGGAGCCGATCCGCTATGTCGGCGCGCACGTCGTGCGCAA
CGCGATCCGCCGCAAGGAGCGCGCGGAGGACGAGAGCCGGCAGCCGTCAGTGGTCGATACGTGGCTCGCGAAATTCGCGA
GCGCGGCGGGGAAGGCGGACAAGGCGTGA

Upstream 100 bases:

>100_bases
GCCGCCGCCGTCCATCGCCGTGCGGCGGTTTCGTTTTCGTTTTTTGCTTTCCCGTCGTGCGCCGCACGGCAATCTTCGAC
CCTTACCGGCATGGCACCCG

Downstream 100 bases:

>100_bases
CGAAACGCTGAACGGAACGCACAACGAAAAAGGGACGCGGCGACGCGTCCCTTTGCCGGTGCGCGGCAGCCCGCGCGGGC
ATTGACCGACGCTTACGCGC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQHDPALDIAILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSGAQVGTLAPVLDALAACGIHSYEPLPSAEV
ARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFTPGQPAVVRTPSGSVTAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLARTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRQPSVVDTWLAKFASAAGKADKA

Sequences:

>Translated_462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQHDPALDIAILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSGAQVGTLAPVLDALAACGIHSYEPLPSAEV
ARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFTPGQPAVVRTPSGSVTAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLARTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRQPSVVDTWLAKFASAAGKADKA
>Mature_462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQHDPALDIAILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSGAQVGTLAPVLDALAACGIHSYEPLPSAEV
ARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFTPGQPAVVRTPSGSVTAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLARTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRQPSVVDTWLAKFASAAGKADKA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=379, Percent_Identity=30.6068601583113, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 49762; Mature: 49762

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQHDPALDIAILEADL
CCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEHH
CGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELR
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LDGTLYTATSGAQVGTLAPVLDALAACGIHSYEPLPSAEVARRSGSARNLDGVYSPIAAT
ECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHCHHHC
VHPGKLVRGLRRVALAMGIRIYERTPMLDFTPGQPAVVRTPSGSVTAGKLVFAINAWMAS
CCCHHHHHHHHHHHHHHCCEEEECCCCEEECCCCCEEEECCCCCCCCCCEEEEEHHHHHH
RFPQFERTIAVVSSDMVITEKCPELLARTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKG
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHCCEEEEEEEEECCCCEEEEECC
GNTFSWRSRIAPVFDRRSPYEAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGR
CCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCCC
LDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGHFPPEPIRYV
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
GAHVVRNAIRRKERAEDESRQPSVVDTWLAKFASAAGKADKA
HHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQHDPALDIAILEADL
CCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEHH
CGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELR
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LDGTLYTATSGAQVGTLAPVLDALAACGIHSYEPLPSAEVARRSGSARNLDGVYSPIAAT
ECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHCHHHC
VHPGKLVRGLRRVALAMGIRIYERTPMLDFTPGQPAVVRTPSGSVTAGKLVFAINAWMAS
CCCHHHHHHHHHHHHHHCCEEEECCCCEEECCCCCEEEECCCCCCCCCCEEEEEHHHHHH
RFPQFERTIAVVSSDMVITEKCPELLARTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKG
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHCCEEEEEEEEECCCCEEEEECC
GNTFSWRSRIAPVFDRRSPYEAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGR
CCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCCC
LDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGHFPPEPIRYV
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
GAHVVRNAIRRKERAEDESRQPSVVDTWLAKFASAAGKADKA
HHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]