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The map label for this gene is dhaAF [H]

Identifier: 116691155

GI number: 116691155

Start: 3454208

End: 3455023

Strand: Direct

Name: dhaAF [H]

Synonym: Bcen2424_3137

Alternate gene names: 116691155

Gene position: 3454208-3455023 (Clockwise)

Preceding gene: 116691153

Following gene: 116691161

Centisome position: 99.15

GC content: 67.89

Gene sequence:

>816_bases
ATGCTCGAACTCCCGAACCGCTTCAATTTCGAAGGCCACCGGATTGCATGGGGCACGCTCGGCGAAGGCCCGCCGCTCGT
CCTCGTGCACGGCACGCCGTTTTCGTCGCAGGTCTGGCGCCGGATCGCGCCGTGGCTGGCGCGGCGCCATCGCGTGTACT
TCTACGACCTGCTCGGCTACGGCCAGTCCGACATGCCGGATGCGGACGTGTCGCTGGGTTGCCAGAACGTGCTGTTCGGC
GCGCTGCTGAATGAATGGAAACTGGCGCGGCCGCGCGTGCTCGCGCACGACTACGGCGGCGCAACCGTGCTGCGCGCGCA
TTTTCTCGACGGCATCGCGTATGCGGATCTGACGCTCGTGAACCCGGTCGCGATCGCGCCGCAAGGCTCGCCGTTCGTGC
GCCACGTCGCGCAGCACGAAGCTGCGTTCACCGGCTTGCCCGCGTACGCACACCATGCGCTCGTGTCGACGTATATCGGC
CAGGCAGTCGCACGGCCGTTGAGCGACGACGTGCTGTCGATCTACCGCGAGCCGTGGCTCACGTCGGCCGGCCAGGCCGC
GTTCTATCGCCAGATCGCGCAGATGCGCCAGCGCTACATCGAGGAAGCGGAGGCCCGCTACGCGCCGCCGGACTTTCCGG
TGCGCATCGTGTGGGGCGAGGACGACGTGTGGATTCCGCTCGAACAAGGGCAGGCGCTCGCCGATCGCATCGCGGACGGC
CAGTTGATCCGGGTCCCGCACGCGGGCCATCTGGTTCAGGAGGATGCGCCGGAAGCAATCGTCGCCGCCGTGCTCGACGG
ATACACGCTGGGCTGA

Upstream 100 bases:

>100_bases
GGTCGGATCGAGTAACCATTTATTTTCGCCTATCGGTTTCACATCCGGAGAATCCGGCGTAACCTGTTAACAACCGAAAC
AGGTTATCACGGAGAAGCCG

Downstream 100 bases:

>100_bases
GCATCACCCCAGCGCGTGTTACGTGCCGCGTCAGAACGCCGGCACGCCTTGCGCGAGCGTCGCGACGAACTTCGCGGTGC
GCGGGTCGCGCGGCCGGCCG

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLELPNRFNFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVYFYDLLGYGQSDMPDADVSLGCQNVLFG
ALLNEWKLARPRVLAHDYGGATVLRAHFLDGIAYADLTLVNPVAIAPQGSPFVRHVAQHEAAFTGLPAYAHHALVSTYIG
QAVARPLSDDVLSIYREPWLTSAGQAAFYRQIAQMRQRYIEEAEARYAPPDFPVRIVWGEDDVWIPLEQGQALADRIADG
QLIRVPHAGHLVQEDAPEAIVAAVLDGYTLG

Sequences:

>Translated_271_residues
MLELPNRFNFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVYFYDLLGYGQSDMPDADVSLGCQNVLFG
ALLNEWKLARPRVLAHDYGGATVLRAHFLDGIAYADLTLVNPVAIAPQGSPFVRHVAQHEAAFTGLPAYAHHALVSTYIG
QAVARPLSDDVLSIYREPWLTSAGQAAFYRQIAQMRQRYIEEAEARYAPPDFPVRIVWGEDDVWIPLEQGQALADRIADG
QLIRVPHAGHLVQEDAPEAIVAAVLDGYTLG
>Mature_271_residues
MLELPNRFNFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVYFYDLLGYGQSDMPDADVSLGCQNVLFG
ALLNEWKLARPRVLAHDYGGATVLRAHFLDGIAYADLTLVNPVAIAPQGSPFVRHVAQHEAAFTGLPAYAHHALVSTYIG
QAVARPLSDDVLSIYREPWLTSAGQAAFYRQIAQMRQRYIEEAEARYAPPDFPVRIVWGEDDVWIPLEQGQALADRIADG
QLIRVPHAGHLVQEDAPEAIVAAVLDGYTLG

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 30079; Mature: 30079

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELPNRFNFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVYFYDLLGY
CCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECC
GQSDMPDADVSLGCQNVLFGALLNEWKLARPRVLAHDYGGATVLRAHFLDGIAYADLTLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEE
NPVAIAPQGSPFVRHVAQHEAAFTGLPAYAHHALVSTYIGQAVARPLSDDVLSIYREPWL
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TSAGQAAFYRQIAQMRQRYIEEAEARYAPPDFPVRIVWGEDDVWIPLEQGQALADRIADG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHHCCHHHHHHHCCC
QLIRVPHAGHLVQEDAPEAIVAAVLDGYTLG
CEEECCCCCCHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLELPNRFNFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVYFYDLLGY
CCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECC
GQSDMPDADVSLGCQNVLFGALLNEWKLARPRVLAHDYGGATVLRAHFLDGIAYADLTLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCEEEE
NPVAIAPQGSPFVRHVAQHEAAFTGLPAYAHHALVSTYIGQAVARPLSDDVLSIYREPWL
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TSAGQAAFYRQIAQMRQRYIEEAEARYAPPDFPVRIVWGEDDVWIPLEQGQALADRIADG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHHCCHHHHHHHCCC
QLIRVPHAGHLVQEDAPEAIVAAVLDGYTLG
CEEECCCCCCHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]