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The map label for this gene is gatA [H]

Identifier: 116691126

GI number: 116691126

Start: 3421850

End: 3423340

Strand: Reverse

Name: gatA [H]

Synonym: Bcen2424_3108

Alternate gene names: 116691126

Gene position: 3423340-3421850 (Counterclockwise)

Preceding gene: 116691127

Following gene: 116691125

Centisome position: 98.26

GC content: 69.28

Gene sequence:

>1491_bases
ATGCACGCAAAAAGCCTGACCGAACTGCGCGCCGCGCTCGCCGCCAAGGAATGCTCGGCCGTCGAGCTCGCGCAGCACTA
TCTGAAACGGATCGACGCCGCGCGCGACCTGAACGCGTTCGTCCACGTCGACGCCGACCTCACCCTCGCGCAGGCGAAAG
CCGCCGACGCCGAACTCGCGCGCGGCGCGGGCGGCGCGCTCACCGGCCTGCCGATCGCGCACAAGGACGTGTTCGTCACG
CGCGGCTGGCGCTCGACCGCCGGCTCGAAAATGCTCGCGAACTACGAGAGCCCGTTCGACGCGACCGTCGTCGCCCGCCT
GCAGGCGGCCGGCATGGTCACGCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAAAACTCGGCGT
TCGGCGCGGTGAAGAACCCGTGGGACACGAACGCGGTGCCGGGCGGCAGCTCGGGCGGCAGCTCGGCCGCCGTCGCCGCG
CGCCTCGCGCCGGCCGCGACCGGCACCGACACCGGCGGCTCGATTCGCCAGCCCGCGTCGTTCGCGGGCGTGACGGGCAT
CAAGCCGACCTACGGCCGCGTGTCGCGCTACGGGATGATCGCGTTCGCGTCGTCGCTCGACCAGGGCGGCCCGATGGCGC
AGAGCGCGTCCGACTGCGCGCTGCTGCTGAACGCGATGGCCGGCTTCGACGAGCGCGACTCGACGAGCCTCGAGCGCGAC
GATGAAGACTTCACGCGCCACCTCGGCCAGCCGTGGGCCGCCGGCAACGACGCGGGCAAGCCGCTCGCGGGCCTGCGCAT
CGGTTTGCCGAACGAGTATTTCGGCGCGGGCCTCGCCGACGACGTGCGCGCGACGATCGACGCGGCCCTCAAGCAATACG
AGGCGCTCGGCGCGACGCTCGTGCCGGTGTCGCTGCCGAAGACGGAACTGTCGATCCCCGTGTACTACGTGATCGCCCCG
GCGGAAGCGTCGTCGAACCTGTCGCGTTTCGACGGCGTGCGCTTCGGCCACCGCGCCGCGCAGTACGGCGATCTGCTCGA
CATGTACAAGAAGTCGCGCGCCGAAGGCTTCGGGCCTGAAGTGAAGCGCCGGATTCTGGTCGGCACCTACGTGCTGTCGC
ACGGTTACTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGAACGATTTCCAGGAAGCGTTCAAG
TCCTGCGACGTGATCATGGGCCCGGCGTCGCCGACGGTCGCATGGGATCTCGGCGCGAAGGGCGACGATCCGGTGCAGAT
GTATCTCGCGGATATCTACACGCTGTCGGTGAGCCTCGCGGGCCTGCCCGGCATGAGCGTGCCGTGCGGCTTCGGCGCGG
GCGCGAACGCGAAGCGCCCGGTCGGCCTGCAGATCATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGAC
GCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGTTCCGGCAGGGGTGTAA

Upstream 100 bases:

>100_bases
GCCTCTATCTCGTGCCGAAGGTGATCGAGTAAGCATTCGATGACAAGCGCGCCGGCCGTCCTGCGCCGCGCGCCACCCAG
AATTCCAGGAAAACCACTCA

Downstream 100 bases:

>100_bases
GCACATGGCTACTCAGTGGGAAGTCGTTATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGG
GCGCATCGACGCAGTTCGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGAGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Sequences:

>Translated_496_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGAGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV
>Mature_496_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGAGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=37.3046875, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=26.1133603238866, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=38.5185185185185, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=475, Percent_Identity=37.0526315789474, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=31.9327731092437, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.7291666666667, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=320, Percent_Identity=27.1875, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=29.6943231441048, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=32.2916666666667, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=32.2916666666667, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=444, Percent_Identity=33.7837837837838, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=28.5035629453682, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=27.4261603375527, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.7959183673469, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24644968, Length=521, Percent_Identity=24.3761996161228, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161078093, Length=531, Percent_Identity=25.2354048964218, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=29.8319327731092, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52378; Mature: 52378

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVDADLTLAQAKAADAELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
RGAGGALTGLPIAHKDVFVTRGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGAGLADDVRATIDAALKQYEALGATL
CHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVDADLTLAQAKAADAELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
RGAGGALTGLPIAHKDVFVTRGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGAGLADDVRATIDAALKQYEALGATL
CHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA