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The map label for this gene is gatB

Identifier: 116691125

GI number: 116691125

Start: 3420370

End: 3421845

Strand: Reverse

Name: gatB

Synonym: Bcen2424_3107

Alternate gene names: 116691125

Gene position: 3421845-3420370 (Counterclockwise)

Preceding gene: 116691126

Following gene: 116691124

Centisome position: 98.22

GC content: 65.24

Gene sequence:

>1476_bases
ATGGCTACTCAGTGGGAAGTCGTTATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGGGCGC
ATCGACGCAGTTCGGCGCGGAGCCGAACACGCAGGCGTGCCCGGTCGACCTGGCGTTGCCGGGCGTGCTGCCGGTGCTGA
ACCGCGGCGCGGTCGAGCGGGCGATCCGCTTCGGCCTCGCGATCGGCTCGACCATCGCGCCGCGCAGCATCTTCGCGCGC
AAGAATTATTTCTACCCCGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCCAGAT
CACGATCCAGGTGCCGGCCAACGAGAAGGCCGGCAAGCCGGCGTACGAGAAGACGGTCAACCTGACCCGCGCGCACCTCG
AGGAAGACGCGGGCAAGTCGCTGCATGAAGACTTCGCCGGCATGACCGGCATCGACCTGAACCGCGCGGGCACGCCGCTG
CTCGAGATCGTCACCGAGCCGGAAATGCGCAGCGCGGCCGAGGCCGTGGCCTATGCGAAGGCGCTGCACGCGCTCGTCGT
GTGGCTCGGCATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCGAACGTGTCGGTGCGACCGGTCGGCC
AGGAGAAATTCGGCACGCGCGCGGAAATCAAGAACCTGAACTCGTTCCGGTTCCTCGAGGAAGCGATCAACTACGAAGTG
CGTCGCCAGATCGAGCTGATCGAGGACGGTGGCGAAGTCGTGCAGGAAACCCGCCTGTACGACCCGGACAAGCGCGAGAC
GCGTTCGATGCGCAGCAAGGAAGACGCGCACGATTACCGCTACTTCCCCGATCCCGACCTGATGCCGCTCGTGATCGGCC
GGGACTGGGTCGAGCGCGTGCAGTCCGGCATGCCGGAGCTGCCGGCCGCGATGCAGCAGCGCTTCGTCGACGAGTACGGC
GTGTCCGCGTACGACGCGGGCGTGCTGACGTCGAGCAAGGCGATGGCCGCGTACTTCGAGTCGGTGGTCGCGAAGGCCGG
CGCAGCGAATGCGAAGATCGTCGCGAACTGGCTGATGGGCGACGTGTCGTCGCAACTGAACCGCGACGGCATCGAGATCG
ACGCGATTCCGGTGTCGGCCGCGCAGCTCGCGCTGTTGCTGCAGCGCATCGCCGACGGCACGATCTCGAACAAGATCGCG
AAGGAAATCTTCGCGACGATCTGGGACGAGAAGGCGACCGACGAAGGCGCGGCCGACCGCATCATCGACGCGAAGGGCCT
CAAGCAGATCTCCGACACCGGCGCGCTGGAAGCGATCATCGACGAAGTGCTCGCGGCGAACGCGAAATCGGTCGAGGAAT
TCCGCGCCGGCAAGGAAAAGGCGTTCAACGCGCTGATCGGCCAGGCGATGAAGGCGACCAAGGGCAAGGCCAATCCGCAG
CAGGTCAACGAACTGCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
ATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGACGCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGT
TCCGGCAGGGGTGTAAGCAC

Downstream 100 bases:

>100_bases
GCATGCGCGCGCGTCTGAACCGCGCTTGACGACGGGCCGCTCCGAACCAGTTCGGGGCGGCCCGTCGCGTTTGGTGGATG
CGTTATCGTATGCACCACAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQSGMPELPAAMQQRFVDEYG
VSAYDAGVLTSSKAMAAYFESVVAKAGAANAKIVANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIA
KEIFATIWDEKATDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG

Sequences:

>Translated_491_residues
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQSGMPELPAAMQQRFVDEYG
VSAYDAGVLTSSKAMAAYFESVVAKAGAANAKIVANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIA
KEIFATIWDEKATDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG
>Mature_490_residues
ATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQSGMPELPAAMQQRFVDEYGV
SAYDAGVLTSSKAMAAYFESVVAKAGAANAKIVANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAK
EIFATIWDEKATDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQQ
VNELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=39.2, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=465, Percent_Identity=32.258064516129, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.5487571701721, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=34.8088531187123, Blast_Score=325, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BURCA (Q1BSL3)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_622365.1
- ProteinModelPortal:   Q1BSL3
- SMR:   Q1BSL3
- STRING:   Q1BSL3
- GeneID:   4093232
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_2493
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   BCEN331271:BCEN_2493-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53532; Mature: 53401

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGSTIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKP
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCC
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCHHHHHHH
QSGMPELPAAMQQRFVDEYGVSAYDAGVLTSSKAMAAYFESVVAKAGAANAKIVANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAKEIFATIWDEKATDEGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
IIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC
>Mature Secondary Structure 
ATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGSTIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKP
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCC
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCHHHHHHH
QSGMPELPAAMQQRFVDEYGVSAYDAGVLTSSKAMAAYFESVVAKAGAANAKIVANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAKEIFATIWDEKATDEGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
IIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA