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The map label for this gene is gloA [H]

Identifier: 116690726

GI number: 116690726

Start: 2996977

End: 2997360

Strand: Reverse

Name: gloA [H]

Synonym: Bcen2424_2706

Alternate gene names: 116690726

Gene position: 2997360-2996977 (Counterclockwise)

Preceding gene: 116690728

Following gene: 116690720

Centisome position: 86.03

GC content: 60.94

Gene sequence:

>384_bases
ATGCGTTTGCTGCATACGATGCTGCGAGTCGGCGATCTCGACCGCTCGATCAAGTTCTACACCGAATTGCTCGGCATGAA
GCTGCTGCGTCGCGAGGATTATCCGGAAGGCAAGTTCACGCTCGCGTTCGTCGGCTACGAAGACGAGAGCACGGGCACCG
TGATCGAGCTGACCCACAACTGGGAGACGCCGTCCTACGATCTCGGCAACGGTTTCGGCCATCTGGCGGTGGCGGTCGAC
GATGCGTACGCGGCCTGCGAGAAGATCAAGGCGCAAGGCGGCAAGGTCACGCGCGAGGCGGGCCCGATGAAGCACGGCAC
GACCGTGATCGCGTTCGTCGAGGATCCGGACGGCTACAAGATCGAGTTCATCCAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGCTGCATCGGCTGCGCCCCCGGGCTAGGCGCCCCCGGCGAGATTCACCCGACTCCGGTAGAATTTCCGGTTCTGGAAAG
TCCCCACAGGAGTACAACAC

Downstream 100 bases:

>100_bases
GCGCCGGACGAACCGGGCCGCGGCATGCGCCGCGGCAGCGAAAAAGGGCGATGCGGGATAATCCGCATCGCCCTTTTCGT
TGGCGCCGAAGCCGTGCGCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRREDYPEGKFTLAFVGYEDESTGTVIELTHNWETPSYDLGNGFGHLAVAVD
DAYAACEKIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQKK

Sequences:

>Translated_127_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRREDYPEGKFTLAFVGYEDESTGTVIELTHNWETPSYDLGNGFGHLAVAVD
DAYAACEKIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQKK
>Mature_127_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRREDYPEGKFTLAFVGYEDESTGTVIELTHNWETPSYDLGNGFGHLAVAVD
DAYAACEKIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQKK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=40.9395973154362, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=67.7165354330709, Blast_Score=181, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=27.0676691729323, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.8489208633094, Blast_Score=98, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20129743, Length=147, Percent_Identity=39.4557823129252, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920404, Length=136, Percent_Identity=30.1470588235294, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14274; Mature: 14274

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRREDYPEGKFTLAFVGYEDESTGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WETPSYDLGNGFGHLAVAVDDAYAACEKIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKK
EEEEECC
>Mature Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRREDYPEGKFTLAFVGYEDESTGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WETPSYDLGNGFGHLAVAVDDAYAACEKIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQKK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]