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The map label for this gene is ksgA [H]

Identifier: 116690728

GI number: 116690728

Start: 2998739

End: 2999566

Strand: Reverse

Name: ksgA [H]

Synonym: Bcen2424_2708

Alternate gene names: 116690728

Gene position: 2999566-2998739 (Counterclockwise)

Preceding gene: 116690729

Following gene: 116690726

Centisome position: 86.1

GC content: 66.91

Gene sequence:

>828_bases
ATGTCGAACAGCAGACAGCACCAAGGCCATTTCGCGCGCAAGCGCTTCGGGCAGAACTTCCTCGTCGATCACGGCGTGAT
CGACTCGATCGTCGCGACGATCGGCCCCGCGCGCGGCCAGCGGATGGTCGAGATCGGCCCGGGGCTCGGCGCGCTGACCG
GGCCGCTGATCGAGCGTCTCGCGACGCCCGAGTCGCCGCTGCACGCGGTCGAACTCGACCGCGACCTGATCGGGCGCCTG
CAGCAACGCTTCGGCGCGCTGCTGGAGCTGCACGCGGGCGACGCGCTCGCGTTCGACTTCCGGTCGCTCGCGGCGCCCGG
CGACAAGCCGTCGCTGCGGATCGTCGGCAATCTGCCGTACAACATTTCCAGCCCGCTGCTGTTTCACCTGATGACGTTCG
CCGATGCGGTCATCGACCAGCATTTCATGCTGCAGAACGAAGTCGTCGAGCGGATGGTCGCGGAGCCGGGCACGAAGGCG
TTTTCGCGGCTGTCCGTGATGCTGCAGTACCGCTACGTGATGGAAAAGATGCTCGACGTGCCGCCGGAATCGTTCCAGCC
GCCGCCGAAGGTCGATTCGGCGATCGTCCGGATGATTCCGTACGAGCCGCACGAACTGCCGGACGTCGATCCGGTACTGC
TCGGCGAAATCGTCACGGCCGCGTTCTCGCAGCGTCGCAAGATGCTGCGCAATACGCTCGGCGACTACCGCGAGACGATC
GATTTCGATGCGCTCGGCTTCGATCTCGCGCGCCGGGCGGAGGACGTGAGCGTGGCCGAATACGTCGGCGTCGCGCAGGC
GCTGGCGGCGCTGCGCAAGGCCGGATGA

Upstream 100 bases:

>100_bases
TCGCCGCGCTCGATACGGCCGTCACGATGGCGCGCCATCGCCGCGCAGCCTGACGCTGCCGGTTTACCCCATTCGTCGTA
TTTTTCGTTTCATTCAGTCG

Downstream 100 bases:

>100_bases
CGGACGCGCGTGGCGCGAGCCCACCCCCGGCCGTATCGAAGAGGGCTGCCCGACCGGCAGCCTTTTTTATTGCCCGCGCG
TGTTGCGCCGGCCGTCACGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSNSRQHQGHFARKRFGQNFLVDHGVIDSIVATIGPARGQRMVEIGPGLGALTGPLIERLATPESPLHAVELDRDLIGRL
QQRFGALLELHAGDALAFDFRSLAAPGDKPSLRIVGNLPYNISSPLLFHLMTFADAVIDQHFMLQNEVVERMVAEPGTKA
FSRLSVMLQYRYVMEKMLDVPPESFQPPPKVDSAIVRMIPYEPHELPDVDPVLLGEIVTAAFSQRRKMLRNTLGDYRETI
DFDALGFDLARRAEDVSVAEYVGVAQALAALRKAG

Sequences:

>Translated_275_residues
MSNSRQHQGHFARKRFGQNFLVDHGVIDSIVATIGPARGQRMVEIGPGLGALTGPLIERLATPESPLHAVELDRDLIGRL
QQRFGALLELHAGDALAFDFRSLAAPGDKPSLRIVGNLPYNISSPLLFHLMTFADAVIDQHFMLQNEVVERMVAEPGTKA
FSRLSVMLQYRYVMEKMLDVPPESFQPPPKVDSAIVRMIPYEPHELPDVDPVLLGEIVTAAFSQRRKMLRNTLGDYRETI
DFDALGFDLARRAEDVSVAEYVGVAQALAALRKAG
>Mature_274_residues
SNSRQHQGHFARKRFGQNFLVDHGVIDSIVATIGPARGQRMVEIGPGLGALTGPLIERLATPESPLHAVELDRDLIGRLQ
QRFGALLELHAGDALAFDFRSLAAPGDKPSLRIVGNLPYNISSPLLFHLMTFADAVIDQHFMLQNEVVERMVAEPGTKAF
SRLSVMLQYRYVMEKMLDVPPESFQPPPKVDSAIVRMIPYEPHELPDVDPVLLGEIVTAAFSQRRKMLRNTLGDYRETID
FDALGFDLARRAEDVSVAEYVGVAQALAALRKAG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=242, Percent_Identity=31.8181818181818, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI7657198, Length=192, Percent_Identity=32.2916666666667, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=53.8745387453875, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25141369, Length=266, Percent_Identity=27.4436090225564, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=199, Percent_Identity=31.1557788944724, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358017, Length=247, Percent_Identity=32.3886639676113, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357273, Length=268, Percent_Identity=29.1044776119403, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30438; Mature: 30306

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSRQHQGHFARKRFGQNFLVDHGVIDSIVATIGPARGQRMVEIGPGLGALTGPLIERL
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
ATPESPLHAVELDRDLIGRLQQRFGALLELHAGDALAFDFRSLAAPGDKPSLRIVGNLPY
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCC
NISSPLLFHLMTFADAVIDQHFMLQNEVVERMVAEPGTKAFSRLSVMLQYRYVMEKMLDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PPESFQPPPKVDSAIVRMIPYEPHELPDVDPVLLGEIVTAAFSQRRKMLRNTLGDYRETI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFDALGFDLARRAEDVSVAEYVGVAQALAALRKAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNSRQHQGHFARKRFGQNFLVDHGVIDSIVATIGPARGQRMVEIGPGLGALTGPLIERL
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
ATPESPLHAVELDRDLIGRLQQRFGALLELHAGDALAFDFRSLAAPGDKPSLRIVGNLPY
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCCC
NISSPLLFHLMTFADAVIDQHFMLQNEVVERMVAEPGTKAFSRLSVMLQYRYVMEKMLDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PPESFQPPPKVDSAIVRMIPYEPHELPDVDPVLLGEIVTAAFSQRRKMLRNTLGDYRETI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFDALGFDLARRAEDVSVAEYVGVAQALAALRKAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA