Click here to switch to the map view.

The map label for this gene is hutC [H]

Identifier: 116690192

GI number: 116690192

Start: 2418518

End: 2419213

Strand: Reverse

Name: hutC [H]

Synonym: Bcen2424_2171

Alternate gene names: 116690192

Gene position: 2419213-2418518 (Counterclockwise)

Preceding gene: 116690193

Following gene: 116690191

Centisome position: 69.44

GC content: 66.95

Gene sequence:

>696_bases
ATGAGTGCGCCGGTCTACCAGGAGATCAAGGATTTCATCCTGGCCCGCATCCATGCCGGCGAGTGGGAGGAGGGCGACCA
GGTGCCGTCCGAGAACGAGCTGGCGCGCGAGTTCAAGGTGGCGCGCATGACCGTCAACCGCGCGCTGCGCGAGTTGACGG
CAGAGCAGGTGCTCACGCGCATGAAGGGCGCGGGCACCTACGTCGCGCGTCCGAAGTACGAGTCGACGCTGGTGGCGATC
CGCAGCATCTCCGAGGAAGTCGGCGCGCGCGGGCATGCATACCGCGCGAGCGTGCTCGGGCTCGACACGATCCGCGCCGA
CGAAGCCCTCGCCGACGAGATGCAGGTGGCCGTGCGCACGAAGCTGTTTCATTCGCAGGTGCTGCACTTCGAGAACGACG
AACCGGTGCAGCTCGAAGAACGGTGGGTGAATCCGGCGGTCGCGCCGGATTACGCCGAGCAGGATTTCACGAACACGACG
CCGAACCAGTACCTGATGCGCGCGGCGCCGCTGCAGCGCGTCGAGTACCGGATCGAAGCGGCGGCCCCGGCGCCGGAGCG
ACGCGAGCAGCTGCGGATGGACGGCGTCGAGCCGTGTCTGGTTTTACATCGGCGCACCTGGTCGCAGGGCGTCGTCGCCT
CGGTGGCGAATTTGTGGCATCCCGGCAGCCGTTATCGCTTCACCGGGCATTTCTGA

Upstream 100 bases:

>100_bases
CACTACTTCGCACCGGACATCGCGGTGATCGCGAAGCTGGTCGGCGAGCGCGCGTTCGCGAAGGTCGCGCCGTTCTCGTT
CGCGTCGGAACAGTAAGCCG

Downstream 100 bases:

>100_bases
TTCCTATTTGATCTTCAATTTCCTTCGGGAGCAGTCGTCATGAACCATCCGAAACACATCGATCCCCGTCTCGATCCGAC
GCGCACGATCCGCGCGCCGC

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSAPVYQEIKDFILARIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVGARGHAYRASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTT
PNQYLMRAAPLQRVEYRIEAAAPAPERREQLRMDGVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Sequences:

>Translated_231_residues
MSAPVYQEIKDFILARIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVGARGHAYRASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTT
PNQYLMRAAPLQRVEYRIEAAAPAPERREQLRMDGVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
>Mature_230_residues
SAPVYQEIKDFILARIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAIR
SISEEVGARGHAYRASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTP
NQYLMRAAPLQRVEYRIEAAAPAPERREQLRMDGVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=199, Percent_Identity=29.6482412060301, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082252, Length=149, Percent_Identity=26.1744966442953, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788418, Length=210, Percent_Identity=25.2380952380952, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790118, Length=139, Percent_Identity=24.4604316546763, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26303; Mature: 26172

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVYQEIKDFILARIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVGARGHAYRASVLGLDTIRADEALADEMQVAVRT
HHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTPNQYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
AAPAPERREQLRMDGVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
SAPVYQEIKDFILARIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVGARGHAYRASVLGLDTIRADEALADEMQVAVRT
HHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTPNQYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
AAPAPERREQLRMDGVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203754; 2834335 [H]