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The map label for this gene is xthA [H]

Identifier: 116690166

GI number: 116690166

Start: 2383980

End: 2384756

Strand: Direct

Name: xthA [H]

Synonym: Bcen2424_2145

Alternate gene names: 116690166

Gene position: 2383980-2384756 (Clockwise)

Preceding gene: 116690165

Following gene: 116690171

Centisome position: 68.43

GC content: 66.02

Gene sequence:

>777_bases
ATGAAAATAGCCACCTGGAACGTCAACTCGCTCAACGTCCGCAAACAGCACGTGCTCGACTGGCTCGCGCAAAGCGGCAC
CGACGTCCTGTGCCTGCAGGAACTGAAGCTGCCGGACGAGAAATTCCCGCGCGCCGATCTCGAGGCGGTCGGCTACCGCA
GCTGGTTCACGGGCCAGAAGACCTACAACGGCGTCGCGATCCTCGTGCGCGACACGCTCGCCGTCGACGAATCGGACGTC
GTGCGCAACATCCCCGGCTTCGACGACCCGCAGCAGCGCGTGATCGCCGCGACGGTCGACGGCGTGCGCATCGTGTCCGC
CTACTTCCCGAACGGCCAGGCGCTCGACTCCGACAAGTTCGTCTACAAGATGCAGTGGCTCGACGCGCTGCAGGCATGGC
TGCGCACCGAGCTGCAGCGCTACCCGAAGCTCGCGCTGCTCGGCGACTACAACATCGCGCCGGAAGACCGCGACGTGCAC
GATCCCGCGAAATGGGAAGGCCAGAACCTCGTGTCGCCGCAGGAGCGCGCGCACTTCGCGCAACTGATCGAACTCGGCTT
CGTCGATGCGTTCCGCCGCTTCGAGCAACCCGAGAAAACCTTCACGTGGTGGGACTACCGGATGATGGCGTTCCGCCGCA
ATGCGGGGCTGCGCATCGACCACATCCTGCTGTCGCCGGCGCTCGCGGCGACCTGCGCATCGTGCGAAGTCGATCGCGTG
CCGCGCACGTGGGAACAGCCGTCCGACCACACGCCCGTCGTCGCGATCGTCGGCTGA

Upstream 100 bases:

>100_bases
TCGCGCGACGATGCGTGACCCTGCATGACACCCGCGTTCGCACCCCATGCTCGCGGTAAGATGACGGCCTCCGAATTCAC
GCCGCACGCCCCTCCCGTTC

Downstream 100 bases:

>100_bases
TCGTCCAGGGCCCGCGCGCGATCAGCGCCGCGCGGGCGCCGGCCCGAGATGGGCCCACAGGAAGCCGAATTCGGCCGCAT
CGGCTTCCGCGCGTTCGAGA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRADLEAVGYRSWFTGQKTYNGVAILVRDTLAVDESDV
VRNIPGFDDPQQRVIAATVDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHILLSPALAATCASCEVDRV
PRTWEQPSDHTPVVAIVG

Sequences:

>Translated_258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRADLEAVGYRSWFTGQKTYNGVAILVRDTLAVDESDV
VRNIPGFDDPQQRVIAATVDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHILLSPALAATCASCEVDRV
PRTWEQPSDHTPVVAIVG
>Mature_258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRADLEAVGYRSWFTGQKTYNGVAILVRDTLAVDESDV
VRNIPGFDDPQQRVIAATVDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHILLSPALAATCASCEVDRV
PRTWEQPSDHTPVVAIVG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=30.3370786516854, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=30.3370786516854, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=30.3370786516854, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=36.0902255639098, Blast_Score=157, Evalue=6e-40,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=28.2945736434109, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29530; Mature: 29530

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRADLEAVGYRSWFTGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCHHHHCCHHHCCCCC
TYNGVAILVRDTLAVDESDVVRNIPGFDDPQQRVIAATVDGVRIVSAYFPNGQALDSDKF
CCCCEEEEEEECHHCCCHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCCCCCCCCCH
VYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHILLSPALAATCASCEVDRV
HHHHHHHHHHHHHHCCCCCCEEHHHHHEEEHHCCCCCEEEHHHHHHHHHHHHHCCCCCCC
PRTWEQPSDHTPVVAIVG
CCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRADLEAVGYRSWFTGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCHHHHCCHHHCCCCC
TYNGVAILVRDTLAVDESDVVRNIPGFDDPQQRVIAATVDGVRIVSAYFPNGQALDSDKF
CCCCEEEEEEECHHCCCHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCCCCCCCCCH
VYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHILLSPALAATCASCEVDRV
HHHHHHHHHHHHHHCCCCCCEEHHHHHEEEHHCCCCCEEEHHHHHHHHHHHHHCCCCCCC
PRTWEQPSDHTPVVAIVG
CCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]