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The map label for this gene is yagI [H]

Identifier: 116690153

GI number: 116690153

Start: 2363531

End: 2364340

Strand: Direct

Name: yagI [H]

Synonym: Bcen2424_2132

Alternate gene names: 116690153

Gene position: 2363531-2364340 (Clockwise)

Preceding gene: 116690140

Following gene: 116690155

Centisome position: 67.84

GC content: 68.64

Gene sequence:

>810_bases
ATGAGCCAACCCACCCCGGATTCAAAAACGTCGATCCAGGTGATCGAACGCATGATGCGGTTGCTGGACGCGCTCGCCGC
GCACAGCGACCCTGTCAGCCTGAAGGAACTTGCGCAGCGCACGGAGCTGCACCCGTCGACCGCGCACCGCATCCTCAACG
ACATGGTGACCTGCCGCCTCGTCGACCGCTCCGATCCCGGCACCTACCGCCTCGGCATGCGGCTGCTCGAGCTCGGCAAC
CTCGTGAAGGCACGCCTGTCGGTGCGCGACGCGGCGCTGATGCCGATGCGCGAGCTGCACCGCCTCACCGGGCAGACCGT
GAACCTGTCGGTGCGCCAGGGCGACGAGATCGTCTATATCGAGCGCGCGTACTCCGAGCGCTCTGGAATGCAAGTCGTCC
GCGCGATCGGCGGCCGCGCGCCGCTGCACCTCACGTCGGTCGGCAAGCTGTTCCTCGCAGCCGACGAAACGTCGCGCGTG
CGGGCCTATGCGACGCGCACCGGGCTGTCCGGCCATACGCAGAACAGCATCACCGACATCGCGAAGCTCGAGCGCGAGCT
GACGATCGTCCGCCAGCAATCGTGCGCGCGCGACAACGAGGAGCTCGAACTCGGCGTGCGCTGCATCGCGGCCGGCATCT
ACGACGATTCGGGCAAGCTCGTCGCGGGCCTGTCGCTGTCGGCGCCGGCCGACCGCCTGCAGGACGCGTGGCTCGGCCAG
TTGAGCCGGACGGCGCTCACCATCTCGGAATCGCTCGGCTACCGGCCGGCGCCCGAAAAGGACGTGGACGGGTTGCAGCG
GCAGGCGTGA

Upstream 100 bases:

>100_bases
CGCGAAACGAGGACTTCCTCGAACGGCGTTCGCTTTTTTCACGATACGAGATACAATCGTTCCAACACCGCTTGACGCGT
CAACCGATTCCTCCCCCGAC

Downstream 100 bases:

>100_bases
GCCGCGCCGTCGGCCCCGCTTCCGGCTCCGATTCCGGTTTCGCCGGATAAAAAAAGCCCCGCGATTGCGGGGCTTTTTCG
TTGCAGCAACCGGTGCGGTA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSQPTPDSKTSIQVIERMMRLLDALAAHSDPVSLKELAQRTELHPSTAHRILNDMVTCRLVDRSDPGTYRLGMRLLELGN
LVKARLSVRDAALMPMRELHRLTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADETSRV
RAYATRTGLSGHTQNSITDIAKLERELTIVRQQSCARDNEELELGVRCIAAGIYDDSGKLVAGLSLSAPADRLQDAWLGQ
LSRTALTISESLGYRPAPEKDVDGLQRQA

Sequences:

>Translated_269_residues
MSQPTPDSKTSIQVIERMMRLLDALAAHSDPVSLKELAQRTELHPSTAHRILNDMVTCRLVDRSDPGTYRLGMRLLELGN
LVKARLSVRDAALMPMRELHRLTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADETSRV
RAYATRTGLSGHTQNSITDIAKLERELTIVRQQSCARDNEELELGVRCIAAGIYDDSGKLVAGLSLSAPADRLQDAWLGQ
LSRTALTISESLGYRPAPEKDVDGLQRQA
>Mature_268_residues
SQPTPDSKTSIQVIERMMRLLDALAAHSDPVSLKELAQRTELHPSTAHRILNDMVTCRLVDRSDPGTYRLGMRLLELGNL
VKARLSVRDAALMPMRELHRLTGQTVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADETSRVR
AYATRTGLSGHTQNSITDIAKLERELTIVRQQSCARDNEELELGVRCIAAGIYDDSGKLVAGLSLSAPADRLQDAWLGQL
SRTALTISESLGYRPAPEKDVDGLQRQA

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=256, Percent_Identity=33.203125, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788131, Length=259, Percent_Identity=33.5907335907336, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789999, Length=250, Percent_Identity=30, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786715, Length=261, Percent_Identity=30.6513409961686, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082359, Length=237, Percent_Identity=30.379746835443, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790752, Length=232, Percent_Identity=28.0172413793103, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788581, Length=253, Percent_Identity=25.296442687747, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29639; Mature: 29508

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPTPDSKTSIQVIERMMRLLDALAAHSDPVSLKELAQRTELHPSTAHRILNDMVTCRL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEE
VDRSDPGTYRLGMRLLELGNLVKARLSVRDAALMPMRELHRLTGQTVNLSVRQGDEIVYI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCEEEEE
ERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADETSRVRAYATRTGLSGHTQNSITDI
EECCCCHHHHHHHHHHCCCCCEEEHHHCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHH
AKLERELTIVRQQSCARDNEELELGVRCIAAGIYDDSGKLVAGLSLSAPADRLQDAWLGQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
LSRTALTISESLGYRPAPEKDVDGLQRQA
HHHHHHHHHHHCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SQPTPDSKTSIQVIERMMRLLDALAAHSDPVSLKELAQRTELHPSTAHRILNDMVTCRL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHEEEE
VDRSDPGTYRLGMRLLELGNLVKARLSVRDAALMPMRELHRLTGQTVNLSVRQGDEIVYI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCEEEEE
ERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADETSRVRAYATRTGLSGHTQNSITDI
EECCCCHHHHHHHHHHCCCCCEEEHHHCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHH
AKLERELTIVRQQSCARDNEELELGVRCIAAGIYDDSGKLVAGLSLSAPADRLQDAWLGQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
LSRTALTISESLGYRPAPEKDVDGLQRQA
HHHHHHHHHHHCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]