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The map label for this gene is carA [H]

Identifier: 116689312

GI number: 116689312

Start: 1418787

End: 1419929

Strand: Direct

Name: carA [H]

Synonym: Bcen2424_1290

Alternate gene names: 116689312

Gene position: 1418787-1419929 (Clockwise)

Preceding gene: 116689311

Following gene: 116689313

Centisome position: 40.72

GC content: 65.62

Gene sequence:

>1143_bases
TTGCCGTCTTTTTCTCCCGCCTTGCTTGCACTCGCCGACGGCACGGTCTTTCGTGGTTATTCGATCGGGGCCGAAGGCCA
TACGATCGGCGAAGTCGTGTTCAATACCGCGATCACCGGCTATCAGGAAATCCTGACTGATCCGAGCTACTCGCGCCAGA
TCGTCACGCTCACCTATCCGCATATCGGCAACGTCGGCGTCAACGCCGAAGACGTCGAAGCCACGAAAGTCCATGCCGCC
GGCCTGATCATCCGTGATCTGCCGACGCTCGCATCGAACTTCCGCATGGACCGTACGCTCGGCGACTACCTGCGCGACGA
AGGCGTCGTCGCGATCGCCGGCATCGACACCCGCAAGCTGACCCGCATCCTGCGCGACACGGGCGCGCAGAGCGGCTGCA
TCCTGACGGGCTCCGACGACGAAGCGAAGGCGATCGAGCTCGCGCGCTCGTTCGAGGGCCTCGCCGGCATGGATCTCGCG
AAGGTCGTGTCGACCACCAAGCCGTTCGAGTGGAAGCAGACGGAATGGCGTCTCGAAGGCGGCTACGGCATGCAGGAAGC
GCCGAAGTACCGCGTCGTCGCGTACGACTTCGGCGTCAAGTACAACATCCTGCGCATGCTCGCGGAGCGCGGCTGCCACG
TGACGGTGCTGCCGGCCCAGGCGAGCGCGGAAGATGCGCTCGCGCTGAATCCGGACGGCATCTTCCTGTCGAACGGCCCC
GGCGACCCCGAGCCGTGCGATTACGCGATCGCGGCCACCAAGGCATTCATCGAGCGCGGCGTGCCGACCTTCGGCATCTG
CCTCGGCCACCAGATCATGGGTCTCGCGGTCGGCGCAAAGACGCTCAAGATGAAGACGGGCCACCACGGCGCGAACCATC
CGGTGAAGGATCTCGGCGACGGCCGGGTCGTGATCACGTCGCAGAACCACGGCTTCGCGGTCGACGCCGATTCGCTGCCG
GCCAACGCGCGCGTGACGCACGTGTCGCTGTTCGACGGCACGCTGCAGGGCTTCGAGTTGACCGACAAGCCGGCGTTCTG
CTTCCAGGGCCACCCGGAAGCGTCGCCCGGCCCGCACGACATCGGCTATCTGTTCGACCGTTTCACCGCGCTGATGGACG
CGGCGAAGCAGCGCAACGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGTCCAGCTAGCGACCACGCGCGCCCACGCAGCGCGGCGCTCGCGCGGCAGGGTAAACAGGTCGGCAGCAGGGTGT
TCCCCAAGGAGCCTCCCGTG

Downstream 100 bases:

>100_bases
GTCACTGACGCAACCGAAGAACGGGAGATTCACATCATGTTCGGCCACGCACTCGGCATCACGGATATCTGGACCTACGT
GTTCGGCGTGATCTTCATCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNAEDVEATKVHAA
GLIIRDLPTLASNFRMDRTLGDYLRDEGVVAIAGIDTRKLTRILRDTGAQSGCILTGSDDEAKAIELARSFEGLAGMDLA
KVVSTTKPFEWKQTEWRLEGGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCHVTVLPAQASAEDALALNPDGIFLSNGP
GDPEPCDYAIAATKAFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLGDGRVVITSQNHGFAVDADSLP
ANARVTHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRNA

Sequences:

>Translated_380_residues
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNAEDVEATKVHAA
GLIIRDLPTLASNFRMDRTLGDYLRDEGVVAIAGIDTRKLTRILRDTGAQSGCILTGSDDEAKAIELARSFEGLAGMDLA
KVVSTTKPFEWKQTEWRLEGGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCHVTVLPAQASAEDALALNPDGIFLSNGP
GDPEPCDYAIAATKAFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLGDGRVVITSQNHGFAVDADSLP
ANARVTHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRNA
>Mature_379_residues
PSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNAEDVEATKVHAAG
LIIRDLPTLASNFRMDRTLGDYLRDEGVVAIAGIDTRKLTRILRDTGAQSGCILTGSDDEAKAIELARSFEGLAGMDLAK
VVSTTKPFEWKQTEWRLEGGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCHVTVLPAQASAEDALALNPDGIFLSNGPG
DPEPCDYAIAATKAFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLGDGRVVITSQNHGFAVDADSLPA
NARVTHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=38.0208333333333, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=36.1256544502618, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=36.1256544502618, Blast_Score=218, Evalue=8e-57,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=64.9867374005305, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1789760, Length=133, Percent_Identity=30.8270676691729, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=38.4415584415584, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=386, Percent_Identity=37.3056994818653, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=37.9310344827586, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24642586, Length=392, Percent_Identity=40.8163265306122, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45555749, Length=392, Percent_Identity=40.8163265306122, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40795; Mature: 40663

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYP
CCCCCCEEEEEECCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HIGNVGVNAEDVEATKVHAAGLIIRDLPTLASNFRMDRTLGDYLRDEGVVAIAGIDTRKL
CCCCCCCCCCCCHHEEEHHHCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHH
TRILRDTGAQSGCILTGSDDEAKAIELARSFEGLAGMDLAKVVSTTKPFEWKQTEWRLEG
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEC
GYGMQEAPKYRVVAYDFGVKYNILRMLAERGCHVTVLPAQASAEDALALNPDGIFLSNGP
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEECCC
GDPEPCDYAIAATKAFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLGD
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCC
GRVVITSQNHGFAVDADSLPANARVTHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHD
CEEEEEECCCCEEEECCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCCH
IGYLFDRFTALMDAAKQRNA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYP
CCCCCEEEEEECCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HIGNVGVNAEDVEATKVHAAGLIIRDLPTLASNFRMDRTLGDYLRDEGVVAIAGIDTRKL
CCCCCCCCCCCCHHEEEHHHCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHH
TRILRDTGAQSGCILTGSDDEAKAIELARSFEGLAGMDLAKVVSTTKPFEWKQTEWRLEG
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEC
GYGMQEAPKYRVVAYDFGVKYNILRMLAERGCHVTVLPAQASAEDALALNPDGIFLSNGP
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEECCC
GDPEPCDYAIAATKAFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLGD
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCC
GRVVITSQNHGFAVDADSLPANARVTHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHD
CEEEEEECCCCEEEECCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCCH
IGYLFDRFTALMDAAKQRNA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]