Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 116689310

GI number: 116689310

Start: 1415473

End: 1417050

Strand: Direct

Name: mltD [H]

Synonym: Bcen2424_1288

Alternate gene names: 116689310

Gene position: 1415473-1417050 (Clockwise)

Preceding gene: 116689309

Following gene: 116689311

Centisome position: 40.63

GC content: 67.24

Gene sequence:

>1578_bases
ATGCGACTTATATTGAGTGCGATGGTGGTGCTGCTACTCGCCGCTTGTGCGAGCCAGCCCCCTGTCGCCAACAACGCCGC
CGATTCGCAGGCGACGTCCAACTACCTCCGTAAATCAGCCACCGCCAAAGAAACTGTCGACGTCGACAAGCAATCCGTCG
GCGACCTGACCACTGCGGACTCCGATCTCTGGGCGCGCATTCGCCGCGGCTTCCAGATGCCCGACCTGCAGAGCGATCTG
GTCGACATGCAGACGACCTGGTATACGCAGCGTCCCGATTACGTGCAGCGCATGACCGAGCGCTCGCAGAAGTACCTGTA
TCACATCGTCGAGGAGCTCGAGGCGCGTCACATGCCGACCGAGCTCGCGCTGCTGCCGTTCATCGAGTCCGCGTACAACC
CGCAGGCGCTGTCGGTCGCGAAGGCGGCCGGCATGTGGCAGTTCATGCCCGGCACCGGCCGCACGTACAACCTGAAGCGC
AACATGTGGCAGGACGAGCGCCGCGACGTGCTCGCGTCGACGAGCGCCGCGCTCGACTACCTGTCCCGCCTGCACGACAT
GTTCGGCGACTGGTATCTGGCGCTGGCCGCGTACAACTGGGGCGAAGGCAACGTGCAGCGCGCGATCGCGCGCAACCAGG
CGGCCGGCTTGCCGACCGACTACCAGAACCTGCGGATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAGGCGGTG
AAGAACATCATCGCGAGCCCGCAGCAGTACGGCCTGGCGCTGCCGGATATCCCGAACCACCCGTATTTCGTGACGGTCAC
GACGTCGCGCGACATCGACGTGGCGGTGGCCGCGAAGCTCGCGAACCTGCCGCTCGACGAATTCCGCTCGCTGAACCCGT
CGTTCTCGAAGCCCGTGATCCTCGGCGCGACCGAGCCGCAGATCCTGCTGCCGTTCGACAACGCATCCGCGTTCGAGAAG
AGCCTGAAAGCGTACAACGGCCAGCTGTCGTCGTGGACCACCTACACGGTCAGCGAGCGTGCACGCCCGGCCGCGATCGC
CGAGAAGATCGGCGTCGACGCCGATACGCTGATGTCGGTCAACAAGATTCCGGCCGGCATGCGCCTGAAGCCGGGCTCGA
CGATCGTCGTGCCGCGCGGCAATGACGACGACGAGGACATCAGCGCCGACGTCGCCGAAAACGGCGCGCTCGCGATGGAG
CCGGACGTCCCCGATACGCGCAAGATGCTGATCCGCGTGCGCCGCAAGCAGTCGATGGCGGCGATCGCCGGCCGCTATGG
CGTGTCGGTCGGCCAGTTGAAGGCGTGGAACCGTACGCACCGCGATCTCGCGATGCCGGGTCAGGCGCTCGTGCTGCACG
TGCCGGTCGGCCGTTCGGTGCCGGCGGAACCCGGTCCGGAGCGGATCGCGACGTCGACGGGCGGTGCGCACATCGAGCGC
GCGAGCCTGGCGGTGGGCGGCAAGTCGCACGGCGGCAAGCGCGGCGCCGCGAAGTCGTCGGCCCGGCCAGCGGCGAAGGC
CGCGCCCGCGAAAGCCGCGCCGGCCAAGGCCGCCGCGCACAAGGGCAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
AATGTAGGATTTCGCTGAGTTTTCGGTGTTTTTATTGACGTGAAGCACGCACTTCCGTAGTATCGCCTGCAATTTCCAGC
CGTCGGAAGCCGAGATTTTC

Downstream 100 bases:

>100_bases
GCGGCTGCCGCACGCAACGTGGCGGTCGGTGAGGCCCGCGCGATTGCGCTATCATCCGACGAATTCGACGAAACGCCGTC
ACCGAGGTGACGGCGTTTTC

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MRLILSAMVVLLLAACASQPPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQNLRMPNETRNYVPKLQAV
KNIIASPQQYGLALPDIPNHPYFVTVTTSRDIDVAVAAKLANLPLDEFRSLNPSFSKPVILGATEPQILLPFDNASAFEK
SLKAYNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSVNKIPAGMRLKPGSTIVVPRGNDDDEDISADVAENGALAME
PDVPDTRKMLIRVRRKQSMAAIAGRYGVSVGQLKAWNRTHRDLAMPGQALVLHVPVGRSVPAEPGPERIATSTGGAHIER
ASLAVGGKSHGGKRGAAKSSARPAAKAAPAKAAPAKAAAHKGKKK

Sequences:

>Translated_525_residues
MRLILSAMVVLLLAACASQPPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQNLRMPNETRNYVPKLQAV
KNIIASPQQYGLALPDIPNHPYFVTVTTSRDIDVAVAAKLANLPLDEFRSLNPSFSKPVILGATEPQILLPFDNASAFEK
SLKAYNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSVNKIPAGMRLKPGSTIVVPRGNDDDEDISADVAENGALAME
PDVPDTRKMLIRVRRKQSMAAIAGRYGVSVGQLKAWNRTHRDLAMPGQALVLHVPVGRSVPAEPGPERIATSTGGAHIER
ASLAVGGKSHGGKRGAAKSSARPAAKAAPAKAAPAKAAAHKGKKK
>Mature_525_residues
MRLILSAMVVLLLAACASQPPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQNLRMPNETRNYVPKLQAV
KNIIASPQQYGLALPDIPNHPYFVTVTTSRDIDVAVAAKLANLPLDEFRSLNPSFSKPVILGATEPQILLPFDNASAFEK
SLKAYNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSVNKIPAGMRLKPGSTIVVPRGNDDDEDISADVAENGALAME
PDVPDTRKMLIRVRRKQSMAAIAGRYGVSVGQLKAWNRTHRDLAMPGQALVLHVPVGRSVPAEPGPERIATSTGGAHIER
ASLAVGGKSHGGKRGAAKSSARPAAKAAPAKAAPAKAAAHKGKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=458, Percent_Identity=34.4978165938865, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57242; Mature: 57242

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILSAMVVLLLAACASQPPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTTAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHCCCCCCCC
SDLWARIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQNLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHHEECCCCCHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHH
KNIIASPQQYGLALPDIPNHPYFVTVTTSRDIDVAVAAKLANLPLDEFRSLNPSFSKPVI
HHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNASAFEKSLKAYNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSV
EECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGNDDDEDISADVAENGALAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
AIAGRYGVSVGQLKAWNRTHRDLAMPGQALVLHVPVGRSVPAEPGPERIATSTGGAHIER
HHHHHHCCCHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCEEEE
ASLAVGGKSHGGKRGAAKSSARPAAKAAPAKAAPAKAAAHKGKKK
HHEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MRLILSAMVVLLLAACASQPPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTTAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHCCCCCCCC
SDLWARIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQNLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHHEECCCCCHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHH
KNIIASPQQYGLALPDIPNHPYFVTVTTSRDIDVAVAAKLANLPLDEFRSLNPSFSKPVI
HHHHCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNASAFEKSLKAYNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSV
EECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGNDDDEDISADVAENGALAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHH
AIAGRYGVSVGQLKAWNRTHRDLAMPGQALVLHVPVGRSVPAEPGPERIATSTGGAHIER
HHHHHHCCCHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCEEEE
ASLAVGGKSHGGKRGAAKSSARPAAKAAPAKAAPAKAAAHKGKKK
HHEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]