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The map label for this gene is gloB [H]

Identifier: 116689309

GI number: 116689309

Start: 1414500

End: 1415321

Strand: Direct

Name: gloB [H]

Synonym: Bcen2424_1287

Alternate gene names: 116689309

Gene position: 1414500-1415321 (Clockwise)

Preceding gene: 116689305

Following gene: 116689310

Centisome position: 40.6

GC content: 68.98

Gene sequence:

>822_bases
ATGAACGAGCTGGAATACGTGCCGGTTCCGGCATTCGAAGACAACTATATCTGGCTCGTCTCGGACGGCCGCGATGCGAT
CGCCGTCGACCCGGGTGAAGCCGCGCCCGTACGCCGGGTTCTTGCCGAACGAGGCTGGCGGTTGACCGCTATTTTACTCA
CGCACCACCATGCCGACCACGTCGGCGGTGTCGAGGCGCTGCAGCATAGCCAACCGGCCGATGTTCCGCTCACCGTTTAC
GGCCCCGCGGCCGAAGCGATCGGCGTGGTCACGCGACCGCTGTCGGGCGGCGATCGCGTGACGCTCGACGCGCCCGCCGT
CGCGTTCGACGTGCTCGACGTGCCGGGCCATACACGCGGCCACATTGCTTACTTTCAGGCGGCCGGTCAAGGCGCGGCCG
GGCAGGGCAATGCGGTGCCGCACGTGTTCTGCGGCGACACGCTGTTCTCGTGCGGCTGCGGCCGCCTGTTCGAAGGCACG
CCCGCGCAGATGCTCGCGTCGCTCGACGCGCTCGCGGCGCTGCCGGGCGACACCCGCGTGCATTGCGCACACGAATACAC
GCTGTCGAATATCCGCTTCGCGCTCGCGTGCGAGCCGGGCAACGCGGCACTCGCCGCGTGGCGCGACGACGCGCAGGCGC
TGCGTGCGCGCGGCGTGCCGACGCTGCCCACTACGATCGCGCACGAGCGTGCCGTTAACCCGTTCATGCGGGCCGACAGC
GCGGCGATCCACGCGACGCTCGAGGCCGAGCTGCACGAAACGGTGACGGATCGCCTGGCGGCGTTCACGCTGATGCGCGA
ATGGAAAAACCGGTTCCGGTGA

Upstream 100 bases:

>100_bases
GCGCCTGCTCCCAGCCCAGCACGTAACGGCCGGGCGGCGAGTCGGTCCAGGCGGGCCAGTCTATAATTTGACGGTCAGAC
ATAACGATGATTGCGCGCCT

Downstream 100 bases:

>100_bases
CGTTTTCCGGAGGACTCCAAAGCTCAAGCGGTGTCTGTAAAAATTGCTCCAAATGTAGGATTTCGCTGAGTTTTCGGTGT
TTTTATTGACGTGAAGCACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVEALQHSQPADVPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAVAFDVLDVPGHTRGHIAYFQAAGQGAAGQGNAVPHVFCGDTLFSCGCGRLFEGT
PAQMLASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDDAQALRARGVPTLPTTIAHERAVNPFMRADS
AAIHATLEAELHETVTDRLAAFTLMREWKNRFR

Sequences:

>Translated_273_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVEALQHSQPADVPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAVAFDVLDVPGHTRGHIAYFQAAGQGAAGQGNAVPHVFCGDTLFSCGCGRLFEGT
PAQMLASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDDAQALRARGVPTLPTTIAHERAVNPFMRADS
AAIHATLEAELHETVTDRLAAFTLMREWKNRFR
>Mature_273_residues
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVEALQHSQPADVPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAVAFDVLDVPGHTRGHIAYFQAAGQGAAGQGNAVPHVFCGDTLFSCGCGRLFEGT
PAQMLASLDALAALPGDTRVHCAHEYTLSNIRFALACEPGNAALAAWRDDAQALRARGVPTLPTTIAHERAVNPFMRADS
AAIHATLEAELHETVTDRLAAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=271, Percent_Identity=38.3763837638376, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI94538322, Length=271, Percent_Identity=38.3763837638376, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI14150041, Length=231, Percent_Identity=38.961038961039, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI116642887, Length=249, Percent_Identity=35.7429718875502, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI21703352, Length=249, Percent_Identity=35.7429718875502, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=39.4871794871795, Blast_Score=117, Evalue=8e-27,
Organism=Escherichia coli, GI1786406, Length=267, Percent_Identity=43.4456928838951, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=34.469696969697, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=275, Percent_Identity=28.3636363636364, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324614, Length=250, Percent_Identity=30.4, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667711, Length=273, Percent_Identity=38.8278388278388, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI21356335, Length=273, Percent_Identity=38.8278388278388, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24667703, Length=273, Percent_Identity=38.8278388278388, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29247; Mature: 29247

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVEALQHSQPADVPLTVYGPAAEAIGVVTRPLSGGDRVTLDAPAVAFDVLDVPGHTRG
CCHHHHHHCCCCCCCCEEEECCCHHHHHHEECCCCCCCEEEEECCEEEEEEEECCCCCCC
HIAYFQAAGQGAAGQGNAVPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRV
EEEEEEECCCCCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
HCAHEYTLSNIRFALACEPGNAALAAWRDDAQALRARGVPTLPTTIAHERAVNPFMRADS
EEEEEEEECCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCC
AAIHATLEAELHETVTDRLAAFTLMREWKNRFR
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFEDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVEALQHSQPADVPLTVYGPAAEAIGVVTRPLSGGDRVTLDAPAVAFDVLDVPGHTRG
CCHHHHHHCCCCCCCCEEEECCCHHHHHHEECCCCCCCEEEEECCEEEEEEEECCCCCCC
HIAYFQAAGQGAAGQGNAVPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRV
EEEEEEECCCCCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
HCAHEYTLSNIRFALACEPGNAALAAWRDDAQALRARGVPTLPTTIAHERAVNPFMRADS
EEEEEEEECCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCC
AAIHATLEAELHETVTDRLAAFTLMREWKNRFR
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA