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The map label for this gene is bkdA2 [H]

Identifier: 116689266

GI number: 116689266

Start: 1374530

End: 1375570

Strand: Direct

Name: bkdA2 [H]

Synonym: Bcen2424_1244

Alternate gene names: 116689266

Gene position: 1374530-1375570 (Clockwise)

Preceding gene: 116689265

Following gene: 116689267

Centisome position: 39.45

GC content: 67.24

Gene sequence:

>1041_bases
ATGGCGCAACACGAGACAGGGACGGCGACGCAGCCGATGACGATGATCCAGGCGCTGCGCTCCGCGATGGACGTGATGCT
CGGGCGCGACAGCGACGTGGTCGTGTTCGGGCAGGACGTCGGCTATTTCGGCGGCGTGTTCCGCTGTACCGAAGGACTGC
AGAACAAGTACGGCAAGTCGCGCGTGTTCGATGCGCCGATCTCGGAAGGCGGGATCGTCGGCGCGGCGGTCGGGATGGGC
GCCTACGGGTTGCGGCCGGTCTGCGAGATCCAGTTCGCCGACTATTTCTATCCGGCATCCGACCAGATCGTGTCGGAAGG
CGCGCGGCTGCGCTACCGCTCGGCCGGCCAGTTCACCGCGCCGATGACGATCCGGATGCCGTGTGGCGGCGGCATCTACG
GCGGGCAGACGCACAGCCAGAGCCCGGAGGCGATGTTCACGCAGGTGTGCGGGCTGCGCACGGTGATGCCGTCGAATCCG
TACGACGCGAAAGGGCTCTTGATCGCGTCGATCGAGAACGACGATCCGGTGATCTTCCTCGAACCGAAACGGCTGTACAA
CGGGCCGTTCGACGGCCATCACGAACGGCCCGTCACGTCGTGGCTCAAGCATCCGGCGAGCGCGGTGCCGGAAGGCTACT
ACACGGTGCCGCTCGATACGGCCGCCGTCGTGCGGCCCGGCAACGACGTGACGGTGCTCACATATGGCACGACGGTGCAC
GTGTCGCTGGCCGCCGCCGAGGAGACGGGCATCGACGCGGAAGTGATCGACCTGCGCACGCTGTGGCCGCTCGATCTCGA
CACCATCGTCGCGTCGGTGCGCAAGACCGGGCGCTGCGTCGTGGTGCACGAGGCGACGCGCACCTGCGGCTATGGCGCGG
AGCTCGTGTCGCTGGTACAGGAACACTGCTTCTACCACCTCGAAGCGCCGGTCGAGCGCACGACGGGCTGGGACACCCCG
TATCCGCATGCGCAGGAATGGGCGTACTTTCCGGGGCCGACCCGGGTCGGTGAAGCGTTGCGACGCGTGATGGAGGCGTG
A

Upstream 100 bases:

>100_bases
CCGACGACCGGATTCCGTCGCCGGCCTCGATGTTCGACGACGTGTACAAGGAGCTGCCCGCGCACCTGCGCCGTCAGCGC
CAGGAACTGGGAGCGTGATC

Downstream 100 bases:

>100_bases
GATGGGGATTCATGTCATCAAGATGCCGGATATCGGCGAAGGGATCGCCGAGGTCGAGCTGGTGGCCTGGCATGTGGAAG
TCGGGCAGACGATCAAGGAA

Product: transketolase, central region

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAQHETGTATQPMTMIQALRSAMDVMLGRDSDVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPISEGGIVGAAVGMG
AYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNP
YDAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPASAVPEGYYTVPLDTAAVVRPGNDVTVLTYGTTVH
VSLAAAEETGIDAEVIDLRTLWPLDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDTP
YPHAQEWAYFPGPTRVGEALRRVMEA

Sequences:

>Translated_346_residues
MAQHETGTATQPMTMIQALRSAMDVMLGRDSDVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPISEGGIVGAAVGMG
AYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNP
YDAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPASAVPEGYYTVPLDTAAVVRPGNDVTVLTYGTTVH
VSLAAAEETGIDAEVIDLRTLWPLDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDTP
YPHAQEWAYFPGPTRVGEALRRVMEA
>Mature_345_residues
AQHETGTATQPMTMIQALRSAMDVMLGRDSDVVVFGQDVGYFGGVFRCTEGLQNKYGKSRVFDAPISEGGIVGAAVGMGA
YGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPY
DAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPASAVPEGYYTVPLDTAAVVRPGNDVTVLTYGTTVHV
SLAAAEETGIDAEVIDLRTLWPLDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDTPY
PHAQEWAYFPGPTRVGEALRRVMEA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=341, Percent_Identity=46.6275659824047, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI34101272, Length=341, Percent_Identity=46.6275659824047, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=34.9693251533742, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=33.7423312883436, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=45.3731343283582, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17538422, Length=343, Percent_Identity=37.3177842565598, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319698, Length=344, Percent_Identity=35.4651162790698, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI160714828, Length=338, Percent_Identity=45.8579881656805, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI160714832, Length=338, Percent_Identity=45.8579881656805, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=38.7692307692308, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=38.7692307692308, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24650943, Length=97, Percent_Identity=41.2371134020619, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650945, Length=97, Percent_Identity=41.2371134020619, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI160714826, Length=67, Percent_Identity=46.2686567164179, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37704; Mature: 37573

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHETGTATQPMTMIQALRSAMDVMLGRDSDVVVFGQDVGYFGGVFRCTEGLQNKYGKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
RVFDAPISEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTA
EEEECCCCCCCEEEHHHCCCCCCCCCEEEEEEECEECCCCHHHHHCCCCEEEECCCCEEC
PMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIASIENDDPVIFL
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCEEEE
EPKRLYNGPFDGHHERPVTSWLKHPASAVPEGYYTVPLDTAAVVRPGNDVTVLTYGTTVH
CCHHHHCCCCCCCCCCCHHHHHHCCHHHCCCCEEEEECCCEEEEECCCCEEEEEECCEEE
VSLAAAEETGIDAEVIDLRTLWPLDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQ
EEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
EHCFYHLEAPVERTTGWDTPYPHAQEWAYFPGPTRVGEALRRVMEA
HHCEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQHETGTATQPMTMIQALRSAMDVMLGRDSDVVVFGQDVGYFGGVFRCTEGLQNKYGKS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
RVFDAPISEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTA
EEEECCCCCCCEEEHHHCCCCCCCCCEEEEEEECEECCCCHHHHHCCCCEEEECCCCEEC
PMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIASIENDDPVIFL
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCEEEE
EPKRLYNGPFDGHHERPVTSWLKHPASAVPEGYYTVPLDTAAVVRPGNDVTVLTYGTTVH
CCHHHHCCCCCCCCCCCHHHHHHCCHHHCCCCEEEEECCCEEEEECCCCEEEEEECCEEE
VSLAAAEETGIDAEVIDLRTLWPLDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQ
EEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
EHCFYHLEAPVERTTGWDTPYPHAQEWAYFPGPTRVGEALRRVMEA
HHCEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]