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The map label for this gene is bkdA1 [H]

Identifier: 116689265

GI number: 116689265

Start: 1373295

End: 1374527

Strand: Direct

Name: bkdA1 [H]

Synonym: Bcen2424_1243

Alternate gene names: 116689265

Gene position: 1373295-1374527 (Clockwise)

Preceding gene: 116689264

Following gene: 116689266

Centisome position: 39.42

GC content: 67.72

Gene sequence:

>1233_bases
ATGAGCCTGTCAGAGCCATTGCGTCTGCATGTGCCGGAGCCCACCGGGCGCCCGGGCTGCAAGACGGACTTTTCCTATCT
GCATCTATCGCCGGCCGGCGCCGTGCGCCGCCCACCGATCGACGTTGCCGCGGCGGACACCGCCGATCTCGCCCGCAGCC
TCGTGCGCGTGCTCGACGACAACGGCAAGGCCGTCGGCCCGTGGGCCCCCGATCTCGACGATGCGCGGCTGATCGCCGGG
CTGCGCGCGATGCTCAAGACCCGCATTTTCGACGCGCGCATGATGATCGCGCAGCGTCAGAAGAAAATCTCCTTCTACAT
GTTGAGCCTCGGCGAAGAGGCGATCGGCACCGCGCACGCGATGGCGCTGCGCGACGGCGACATGTGCTTTCCGACCTACC
GGCAGCAGAGCATCCTGATCGCGCGCGACGTGCCGCTCGAGCGGATGATCTGCCAGCTGATGTCGAACGAAGGCGATCCG
CTGAAGGGCCGCCAGCTCCCGGTGATGTACTCGGACCGCGAAGCCGGCTTCTTCTCCATTTCCGGCAACCTCGCGACGCA
GTTCATCCAGGCGGTCGGCTGGGCGATGGCGTCGGCGATCAAGGGCGACACGAAGATCGCATCCGCATGGATCGGCGACG
GCGCGACGGCCGAAGCCGATTTCCATACCGCGCTCACGTTCGCGCATGTGTACCGCGCGCCGGTCGTGCTGAACGTGGTC
AACAACCAGTGGGCGATCTCGACGTTCCAGGCGATCGCGGGCGGCGAGGGCACGACCTTCGCGGGGCGCGGCGTCGGTTG
CGGGATCGCGTCGCTGCGCGTCGACGGCAACGACTTCCTCGCGATCTATGCGGCGTCGAGCTGGGCGGCCGAGCGTGCGC
GCCGCAATCTCGGCCCGACGCTGATCGAGTGGGTGACCTACCGCGCGGGCGCGCATTCGACGTCGGACGATCCGACCAAG
TACCGGCCGAGCGACGACTGGTCCCATTTCCCGCTGGGCGATCCGATCGAGCGCTTCAAGCGCCACCTGATCGTGAAGGG
CATCTGGTCGGACAGCGCGCACGACGCGTTGACGGCCGAGCTCGAGGCCGAGGTGATCGCCGCGCAGAAGGAAGCGGAGA
AGTACGGGACGCTGGCCGACGACCGGATTCCGTCGCCGGCCTCGATGTTCGACGACGTGTACAAGGAGCTGCCCGCGCAC
CTGCGCCGTCAGCGCCAGGAACTGGGAGCGTGA

Upstream 100 bases:

>100_bases
GGTTGACGTGCTTATCCGCGCGTCAGCTCGCGCGCAGGCCGGCAACCGGCCCGCGCGTTCCCAAACAGCGTGAGACACAA
TCGAGCGTGGAGACGACACG

Downstream 100 bases:

>100_bases
TCATGGCGCAACACGAGACAGGGACGGCGACGCAGCCGATGACGATGATCCAGGCGCTGCGCTCCGCGATGGACGTGATG
CTCGGGCGCGACAGCGACGT

Product: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAAADTADLARSLVRVLDDNGKAVGPWAPDLDDARLIAG
LRAMLKTRIFDARMMIAQRQKKISFYMLSLGEEAIGTAHAMALRDGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDP
LKGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASSWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTK
YRPSDDWSHFPLGDPIERFKRHLIVKGIWSDSAHDALTAELEAEVIAAQKEAEKYGTLADDRIPSPASMFDDVYKELPAH
LRRQRQELGA

Sequences:

>Translated_410_residues
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAAADTADLARSLVRVLDDNGKAVGPWAPDLDDARLIAG
LRAMLKTRIFDARMMIAQRQKKISFYMLSLGEEAIGTAHAMALRDGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDP
LKGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASSWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTK
YRPSDDWSHFPLGDPIERFKRHLIVKGIWSDSAHDALTAELEAEVIAAQKEAEKYGTLADDRIPSPASMFDDVYKELPAH
LRRQRQELGA
>Mature_409_residues
SLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAAADTADLARSLVRVLDDNGKAVGPWAPDLDDARLIAGL
RAMLKTRIFDARMMIAQRQKKISFYMLSLGEEAIGTAHAMALRDGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPL
KGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVVN
NQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASSWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTKY
RPSDDWSHFPLGDPIERFKRHLIVKGIWSDSAHDALTAELEAEVIAAQKEAEKYGTLADDRIPSPASMFDDVYKELPAHL
RRQRQELGA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=357, Percent_Identity=37.5350140056022, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI258645172, Length=358, Percent_Identity=37.4301675977654, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI4885543, Length=302, Percent_Identity=25.4966887417219, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI291084742, Length=287, Percent_Identity=25.7839721254355, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4505685, Length=287, Percent_Identity=25.7839721254355, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI291084744, Length=262, Percent_Identity=25.5725190839695, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI86563355, Length=393, Percent_Identity=36.3867684478371, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI86563357, Length=393, Percent_Identity=36.3867684478371, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17536047, Length=305, Percent_Identity=26.5573770491803, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32564172, Length=305, Percent_Identity=26.5573770491803, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321026, Length=378, Percent_Identity=29.6296296296296, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355903, Length=363, Percent_Identity=35.2617079889807, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24639748, Length=314, Percent_Identity=26.4331210191083, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24639740, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639746, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24639744, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571106, Length=263, Percent_Identity=26.9961977186312, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 44843; Mature: 44712

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAAADTADLARSLVRVLDD
CCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHCC
NGKAVGPWAPDLDDARLIAGLRAMLKTRIFDARMMIAQRQKKISFYMLSLGEEAIGTAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
MALRDGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPLKGRQLPVMYSDREAGFFSI
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASSWAAERARRNLGPT
CCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHCCCHH
LIEWVTYRAGAHSTSDDPTKYRPSDDWSHFPLGDPIERFKRHLIVKGIWSDSAHDALTAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCCHHHHHH
LEAEVIAAQKEAEKYGTLADDRIPSPASMFDDVYKELPAHLRRQRQELGA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAAADTADLARSLVRVLDD
CCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHCC
NGKAVGPWAPDLDDARLIAGLRAMLKTRIFDARMMIAQRQKKISFYMLSLGEEAIGTAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
MALRDGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPLKGRQLPVMYSDREAGFFSI
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASSWAAERARRNLGPT
CCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHCCCHH
LIEWVTYRAGAHSTSDDPTKYRPSDDWSHFPLGDPIERFKRHLIVKGIWSDSAHDALTAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCCHHHHHH
LEAEVIAAQKEAEKYGTLADDRIPSPASMFDDVYKELPAHLRRQRQELGA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]