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The map label for this gene is gmd [H]

Identifier: 116688903

GI number: 116688903

Start: 984295

End: 985413

Strand: Direct

Name: gmd [H]

Synonym: Bcen2424_0880

Alternate gene names: 116688903

Gene position: 984295-985413 (Clockwise)

Preceding gene: 116688902

Following gene: 116688904

Centisome position: 28.25

GC content: 59.96

Gene sequence:

>1119_bases
ATGTCGCATCGGCCGCTCTCATGGCGTGACCACGATCGACGATTCTGGCCGGAGCTTCAACATGCTGAACAAACTGGAAA
GAACATGACTGTTGCAATCATCACTGGCATTACCGGGCAAGACGGCGCGTATCTTGCGGAGCTGCTTCTCGAGAAGGGCT
ACACCGTCTACGGCACCTATCGGCGCACCAGCTCGGTGAACTTCTGGCGAATCGACGAGCTCGGCGTCAGCAATCATCCT
GATCTTCACCTCGTCGAATACGACCTGACCGATCTTGGCGCAAGCATTCGCCTGCTTCAGACGACGCAAGCGACCGAGGT
CTACAACCTGGCGGCCCAAAGTTTCGTCGGCGTGTCGTTCGATCAGCCGGCAACCACCGCCGAGATTACCGGTATCGGCG
CGCTCAACCTGCTCGAGGCGATTCGCACGGTCAATCCTGCGATCCGCTTCTACCAGGCCAGCACGTCGGAAATGTTCGGC
AAGGTACAGGCCGTACCGCAGGTTGAAAGCACGCCTTTCTATCCGCGCAGTCCGTACGGCGTCGCAAAGCTTTACGCGCA
CTGGATGACGATCAACTATCGCGAGAGCTACAACATCTTCGGCTGCAGCGGCATCCTGTTCAACCACGAATCGCCGCTGC
GCGGGCGCGAATTCGTGACGCGGAAGATTACGGACAGTCTGGCGAAGATCCGTCTCGGCAAGCTCGACGTGCTGGAACTC
GGCAACCTCGACGCCAAGCGTGACTGGGGCTATGCAAAGGAGTACGTCGAAGGCATGTGGCGCATGCTGCAGGCGGAACG
CCCCGATACCTACGTGCTTGCGACGAACCGGACCGAGACGGTGCGGGATTTCGTGTCGATGGCAGCCCAGGCGGCAGGCA
TCGAACTCGCGTGGCAAGGTGCGGGCGAAAACGAGACGGGCGTCGATACCCGAACCGGCAAGGCCGTGGTCAAGGTCAGC
CCGAAATTTTACCGGCCCGCCGAAGTCGATCTGCTCATCGGCAACCCCGAGAAGGCGAGCAAGGAACTCGGCTGGGTGCC
GAAGACGACGCTCGAGCAGCTTTGCCAGATGATGGTCGAGGCGGACATTCGTCGCAACGAAGCAGGCTTCTCGTTCTAA

Upstream 100 bases:

>100_bases
ATCTCGGTGAAGACGACATCGTGCGCCTCGACGACAACTACGGGCGCGATGTCGTCGCGGCGAAGGGCAATTAACCACGA
TTGGAATCCGGCCTGGATTC

Downstream 100 bases:

>100_bases
GGCCATGGCAAAAGTTTTCGTCACGGGCCTGGACGGATTTACCGGCCGCTATATGGCGGAGGAGTTGATCCGCTCGGGGC
ACGAAGTTTGCGGGATCGTT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSHRPLSWRDHDRRFWPELQHAEQTGKNMTVAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIDELGVSNHP
DLHLVEYDLTDLGASIRLLQTTQATEVYNLAAQSFVGVSFDQPATTAEITGIGALNLLEAIRTVNPAIRFYQASTSEMFG
KVQAVPQVESTPFYPRSPYGVAKLYAHWMTINYRESYNIFGCSGILFNHESPLRGREFVTRKITDSLAKIRLGKLDVLEL
GNLDAKRDWGYAKEYVEGMWRMLQAERPDTYVLATNRTETVRDFVSMAAQAAGIELAWQGAGENETGVDTRTGKAVVKVS
PKFYRPAEVDLLIGNPEKASKELGWVPKTTLEQLCQMMVEADIRRNEAGFSF

Sequences:

>Translated_372_residues
MSHRPLSWRDHDRRFWPELQHAEQTGKNMTVAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIDELGVSNHP
DLHLVEYDLTDLGASIRLLQTTQATEVYNLAAQSFVGVSFDQPATTAEITGIGALNLLEAIRTVNPAIRFYQASTSEMFG
KVQAVPQVESTPFYPRSPYGVAKLYAHWMTINYRESYNIFGCSGILFNHESPLRGREFVTRKITDSLAKIRLGKLDVLEL
GNLDAKRDWGYAKEYVEGMWRMLQAERPDTYVLATNRTETVRDFVSMAAQAAGIELAWQGAGENETGVDTRTGKAVVKVS
PKFYRPAEVDLLIGNPEKASKELGWVPKTTLEQLCQMMVEADIRRNEAGFSF
>Mature_371_residues
SHRPLSWRDHDRRFWPELQHAEQTGKNMTVAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIDELGVSNHPD
LHLVEYDLTDLGASIRLLQTTQATEVYNLAAQSFVGVSFDQPATTAEITGIGALNLLEAIRTVNPAIRFYQASTSEMFGK
VQAVPQVESTPFYPRSPYGVAKLYAHWMTINYRESYNIFGCSGILFNHESPLRGREFVTRKITDSLAKIRLGKLDVLELG
NLDAKRDWGYAKEYVEGMWRMLQAERPDTYVLATNRTETVRDFVSMAAQAAGIELAWQGAGENETGVDTRTGKAVVKVSP
KFYRPAEVDLLIGNPEKASKELGWVPKTTLEQLCQMMVEADIRRNEAGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=52.2988505747126, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=51.4367816091954, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=55.8823529411765, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=55.8823529411765, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=55.8823529411765, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=55.8823529411765, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=55.8823529411765, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=55.3314121037464, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24158427, Length=341, Percent_Identity=53.0791788856305, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41867; Mature: 41736

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRPLSWRDHDRRFWPELQHAEQTGKNMTVAIITGITGQDGAYLAELLLEKGYTVYGTY
CCCCCCCCCCCCCHHCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
RRTSSVNFWRIDELGVSNHPDLHLVEYDLTDLGASIRLLQTTQATEVYNLAAQSFVGVSF
EECCCCCEEEEECCCCCCCCCEEEEEECHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCC
DQPATTAEITGIGALNLLEAIRTVNPAIRFYQASTSEMFGKVQAVPQVESTPFYPRSPYG
CCCCCEEEECCCCHHHHHHHHHHCCHHHEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCH
VAKLYAHWMTINYRESYNIFGCSGILFNHESPLRGREFVTRKITDSLAKIRLGKLDVLEL
HHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEC
GNLDAKRDWGYAKEYVEGMWRMLQAERPDTYVLATNRTETVRDFVSMAAQAAGIELAWQG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEEEC
AGENETGVDTRTGKAVVKVSPKFYRPAEVDLLIGNPEKASKELGWVPKTTLEQLCQMMVE
CCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHH
ADIRRNEAGFSF
HHHHCCCCCCCC
>Mature Secondary Structure 
SHRPLSWRDHDRRFWPELQHAEQTGKNMTVAIITGITGQDGAYLAELLLEKGYTVYGTY
CCCCCCCCCCCCHHCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
RRTSSVNFWRIDELGVSNHPDLHLVEYDLTDLGASIRLLQTTQATEVYNLAAQSFVGVSF
EECCCCCEEEEECCCCCCCCCEEEEEECHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCC
DQPATTAEITGIGALNLLEAIRTVNPAIRFYQASTSEMFGKVQAVPQVESTPFYPRSPYG
CCCCCEEEECCCCHHHHHHHHHHCCHHHEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCH
VAKLYAHWMTINYRESYNIFGCSGILFNHESPLRGREFVTRKITDSLAKIRLGKLDVLEL
HHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEC
GNLDAKRDWGYAKEYVEGMWRMLQAERPDTYVLATNRTETVRDFVSMAAQAAGIELAWQG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEEEC
AGENETGVDTRTGKAVVKVSPKFYRPAEVDLLIGNPEKASKELGWVPKTTLEQLCQMMVE
CCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHH
ADIRRNEAGFSF
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]