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The map label for this gene is leuS [H]

Identifier: 116688677

GI number: 116688677

Start: 726316

End: 728910

Strand: Direct

Name: leuS [H]

Synonym: Bcen2424_0654

Alternate gene names: 116688677

Gene position: 726316-728910 (Clockwise)

Preceding gene: 116688676

Following gene: 116688678

Centisome position: 20.85

GC content: 65.97

Gene sequence:

>2595_bases
ATGCACGAGAGATACGTACCCGCCGACGTCGAAGCCGCCGCCCAGGGCGACTGGCGCGCAGCCGATGCCTACAAGACGAA
GGAAGATTCGCAGAAGCCGAAGTTCTACTGCGTATCGATGCTGCCGTACCCGTCCGGCAAGCTGCACATGGGTCACGTGC
GCAACTACACGATCAACGACGTGATGTACCGCTATCTGCGGATGAACGGCTACAACACGCTGATGCCGATGGGCTGGGAT
GCGTTCGGGATGCCGGCCGAGAACGCCGCGATGGCGAACGGCGTGCCGCCCGCGAAGTGGACCTACGACAACATCGACTA
CATGAAGGGCCAGATGCAGTCGATGGGTCTCGCGATCGACTGGTCGCGCGAAATCGCGACGTGCAAGCCCGACTACTACA
AGTGGAACCAGTGGCTGTTCCTGAAGATGCTCGAGAAGGGCATCGCGTACAAGAAGACGGGCACCGTGAACTGGGACCCG
GTCGACCAGACCGTGCTCGCGAACGAGCAGGTGATCGACGGCCGCGGCTGGCGCTCGGGCGCGCTCGTCGAGAAGCGCGA
GATCCCGATGTACTACCTGCGCATCACGCAGTACGCGGATGAGCTGCTGAACGATCTCGACGGCCTCGGCTGGCCCGAGC
GCGTGAAGATCATGCAGCAGAACTGGATCGGCAAGAGCTTCGGCGTGAACTTCGGCTTCCCGTACGAACTCGACGGCGAG
CAGAAGCTGCTGCGCGTGTTCACGACGCGCGCCGACACGATCATGGGCGTCACGTTCTGCGCGATCGCGGCCGAGCACCC
GCTCGCCACGCGCCTCGCGCAGGACAAGCCGGAACTGCTCGCGTTCATCGAGGAATGCAAGCGCGGCGGCGTCGCCGAGG
CCGACGTCGCGACGATGGAGAAGAAGGGCGTCGCGACCGGCTTCTCGGTCAAGCACCCGCTGACCGGCGAGCCGGTCGAG
GTGTGGATCGGCAACTACGTGCTGATGAGCTATGGCGAAGGCGCGGTGATGGGCGTGCCGGGTCACGACGAGCGCGATTT
CGCGTTCGCGAAGAAATACGATCTGCCGATCAGGCAGGTGATCGCGAGCGAAGGGCAGACGTACTCGCTCGACGCATGGC
AGGAGTGGTACGGCGACAAGGAAACCGCGGTCTGCGTGAACAGCGGCAAGTACGACGGCCTGCGCTACGCGGAAGCGGTC
GACGCGGTCGCGGCCGACCTGAAGGCCGGCGGCTTCGGCGACAAGCAGGTCACGTGGCGCCTGCGCGACTGGGGCGTGTC
GCGCCAGCGCTACTGGGGCACGCCGATCCCGATCATCCACTGCCCGTCGTGCGGCGACGTGCCGGTGCCGGAGCAGGATC
TGCCGGTCGTGCTGCCGGAAGACCTCGTGCCGGACGGCTCGGGCAACCCGCTCGCGAAGTCGGAAGCGTTCCTGAACTGC
ACGTGCCCGAAGTGTGGCGCGGCCGCGAAGCGCGAAACCGACACGATGGACACCTTCGTCGATTCGTCGTGGTACTTCTC
GCGCTACACGGCGCCGGACGCCGAGACGATGGTCGACGCGCGCACCGACTACTGGATGCCGATGGACCAGTACATCGGCG
GCATCGAGCACGCGATCCTGCATCTGCTGTATTCGCGCTTCTGGACTAAGGTGATGCGTGATCTCGGCCTCGTGAAGTTC
GGCGAGCCGGCGAAGAACCTGCTCACGCAGGGGATGGTGCTGAACGAGACGTACTACCGCGAAGACGCGGCGGGCAAGAA
GACCTGGTACAACCCGCTCGACGTGACGGTCACGCACGACGACAAGGGCCGCCCGGTCGGCGCGACGCTGAACGCGGACG
GCCAGCCGGTCGTGCTCGGCGGCATCGAGAAGATGTCGAAGTCGAAGAACAACGGCGTCGATCCGCAACTGCTGATCGAC
CAGTACGGCGCCGATACCGCGCGTCTGTTCACGATGTTCGCCGCACCGCCCGAGCAGCAGCTCGAGTGGTCGGGCGCGGG
CGTCGAGGGCGCGAGCCGCTTCCTGCGCCGCGTGTGGAGCTTCGGCTACGGTAACCGCGAAGCGCTCGCCGCGCGCGCCG
GTTTCGACGCCGCGACGCTCGGCGATGCCGACAAGGCGTTGCGCCGCGAGATCTACAGCGTGCTGAAGCAGGCCGATTTC
GACTACCAGCGCCTGCAGTACAACACGGTCGTGTCGGCCGCGATGAAGATGCTGAACGCGATCGACGGCGCAAAGGGTGC
GACGCCCGCCGTGCTGCGCGAGACGTACGGCGTGTTGCTGCGCGTGCTGTATCCGGTCGTGCCGCACGTCACGTTCGAGC
TGTGGAAGACGCTCGGCTACGCGGACGAATTCGGCGCGCTGCTCGATGCGCCGTGGCCGAAGGTCGACGAGGCCGCGCTC
GAGCAGGCCGAGATCGAACTCGTGCTGCAGGTGAACGGCAAGGTGCGCGGCGCGCTGAAGGTCGCGAAGGACGCGAGCCG
CGACGCGATCGAAGCGGCGGCGGTGGCCGACGAGGCATTCGCGAAATTCAGCGACGGCAAGCCGGCGAAGAAGATCGTCG
TCGTGCCGGGCCGCCTCGTGAACATCGTCGTCTGA

Upstream 100 bases:

>100_bases
CCGCCACAGCGAACCTGCAGCGCCGCCGGGCCAGCACGATTTTCGATCCAATCCTGCGCGCGAGCCGTCGCGCCGCTTAA
AGCCTAGCCCGAACCACACC

Downstream 100 bases:

>100_bases
CGGCCGCCGGCCGTCGGACGTACCCAGAAGGAGCGAAGGTGATCCGCAGATCGTTTTTGATGCTCGTCGGCAGCACGGTC
GTGCTGTCGGCATGCGGCTT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MHERYVPADVEAAAQGDWRAADAYKTKEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLAFIEECKRGGVAEADVATMEKKGVATGFSVKHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIRQVIASEGQTYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLKAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
TCPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETYYREDAAGKKTWYNPLDVTVTHDDKGRPVGATLNADGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGYGNREALAARAGFDAATLGDADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKTLGYADEFGALLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASRDAIEAAAVADEAFAKFSDGKPAKKIVVVPGRLVNIVV

Sequences:

>Translated_864_residues
MHERYVPADVEAAAQGDWRAADAYKTKEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLAFIEECKRGGVAEADVATMEKKGVATGFSVKHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIRQVIASEGQTYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLKAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
TCPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETYYREDAAGKKTWYNPLDVTVTHDDKGRPVGATLNADGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGYGNREALAARAGFDAATLGDADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKTLGYADEFGALLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASRDAIEAAAVADEAFAKFSDGKPAKKIVVVPGRLVNIVV
>Mature_864_residues
MHERYVPADVEAAAQGDWRAADAYKTKEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLAFIEECKRGGVAEADVATMEKKGVATGFSVKHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIRQVIASEGQTYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLKAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
TCPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETYYREDAAGKKTWYNPLDVTVTHDDKGRPVGATLNADGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGYGNREALAARAGFDAATLGDADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKTLGYADEFGALLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASRDAIEAAAVADEAFAKFSDGKPAKKIVVVPGRLVNIVV

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=868, Percent_Identity=36.405529953917, Blast_Score=529, Evalue=1e-150,
Organism=Escherichia coli, GI1786861, Length=874, Percent_Identity=49.7711670480549, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=386, Percent_Identity=23.3160621761658, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71997517, Length=267, Percent_Identity=41.1985018726592, Blast_Score=222, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI71997510, Length=267, Percent_Identity=41.1985018726592, Blast_Score=221, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI212645227, Length=338, Percent_Identity=29.2899408284024, Blast_Score=137, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323414, Length=830, Percent_Identity=40.2409638554217, Blast_Score=557, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6321531, Length=957, Percent_Identity=20.7941483803553, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355409, Length=900, Percent_Identity=34.8888888888889, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281366294, Length=482, Percent_Identity=21.1618257261411, Blast_Score=77, Evalue=7e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96258; Mature: 96258

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERYVPADVEAAAQGDWRAADAYKTKEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHHHCCEEEE
WSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
CCEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCH
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLAFIEECKRGGVAEADVATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVKHPLTGEPVEVWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIRQV
HCCCCCCCEECCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCCHHHHHCCCHHHHH
IASEGQTYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAVDAVAADLKAGGFGDKQVTWR
HHCCCCEEECHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
LRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCCEEEE
TCPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWTKVMRDLGLVKFGEPAKNLLTQGMVLNETYYREDAAGKKTWYNPLDVTVTHD
HHHHHHHHHHHHHHCCCEECCCHHHHHHHCCEEECHHHHCCCCCCCCCCCCCEEEEEEEC
DKGRPVGATLNADGQPVVLGGIEKMSKSKNNGVDPQLLIDQYGADTARLFTMFAAPPEQQ
CCCCCEEEEECCCCCEEEEECHHHHHCCCCCCCCHHHHHHHCCCCHHHHHEEECCCCHHH
LEWSGAGVEGASRFLRRVWSFGYGNREALAARAGFDAATLGDADKALRREIYSVLKQADF
CEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIDGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKTLGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGALLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASRDAIEAAAVADEAF
HHHHHHHHCCCCCCCCHHHHHHCCEEEEEEECCCEEEHHHHHHHCCHHHHHHHHHHHHHH
AKFSDGKPAKKIVVVPGRLVNIVV
HHCCCCCCCCEEEECCCCEEEEEC
>Mature Secondary Structure
MHERYVPADVEAAAQGDWRAADAYKTKEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHHHCCEEEE
WSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
CCEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCH
QKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLAFIEECKRGGVAEADVATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVKHPLTGEPVEVWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIRQV
HCCCCCCCEECCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCCHHHHHCCCHHHHH
IASEGQTYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAVDAVAADLKAGGFGDKQVTWR
HHCCCCEEECHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
LRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCCEEEE
TCPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWTKVMRDLGLVKFGEPAKNLLTQGMVLNETYYREDAAGKKTWYNPLDVTVTHD
HHHHHHHHHHHHHHCCCEECCCHHHHHHHCCEEECHHHHCCCCCCCCCCCCCEEEEEEEC
DKGRPVGATLNADGQPVVLGGIEKMSKSKNNGVDPQLLIDQYGADTARLFTMFAAPPEQQ
CCCCCEEEEECCCCCEEEEECHHHHHCCCCCCCCHHHHHHHCCCCHHHHHEEECCCCHHH
LEWSGAGVEGASRFLRRVWSFGYGNREALAARAGFDAATLGDADKALRREIYSVLKQADF
CEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIDGAKGATPAVLRETYGVLLRVLYPVVPHVTFELWKTLGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGALLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASRDAIEAAAVADEAF
HHHHHHHHCCCCCCCCHHHHHHCCEEEEEEECCCEEEHHHHHHHCCHHHHHHHHHHHHHH
AKFSDGKPAKKIVVVPGRLVNIVV
HHCCCCCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA