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The map label for this gene is ysgA [C]

Identifier: 116688300

GI number: 116688300

Start: 318361

End: 319053

Strand: Reverse

Name: ysgA [C]

Synonym: Bcen2424_0276

Alternate gene names: 116688300

Gene position: 319053-318361 (Counterclockwise)

Preceding gene: 116688303

Following gene: 116688299

Centisome position: 9.16

GC content: 69.12

Gene sequence:

>693_bases
GTGACTGCCCAATGGATCGACATCCCGACCGGTAACGACAGCTTCGGCGGCTATCTCGCACTGCCCAAGCGCGGCAAGGG
CCCTGCCGTCATCATCATCCAGGAGATCTTCGGCGTGAACGCGCACATCCGTGCGGTCGCCGACCAGTACGCGGCCGACG
GCTTCGTCGCGCTCGCGCCCGACGTGTTCTGGCGCACGCAGCCGCGCGTCGAGCTGACCTACGAAGGCGCGGATCGCGAC
AAGGGCATCGAGCTGATGAAAAAGACCGATGTGGGCCTCGCGGTCGCGGACATCGGCGCGGCGGCCGACGCGTTGCGCGC
CCGCCCCGAAGTGGCCGGCAAGCTCGCCGCGATCGGCTACTGCTTCGGCGGCCAGCTCGCGTACCGCGCGGCCGCAACCG
GCAAGCTCGACGCGGCAGTGTCCTATTACGGCGGCGGCATCCAGAACGCGCTCGACCTCGCCGGCCAGGTCACGCAGCCG
ATCCTGTTCCACTACGCGGAAAACGACCACGGCATTCCGCTCACCGCCGTCGACCAGGTGAAGGCCGCGTTCGCCGGCCA
CGGCGATGCGTCGTTCCACGTGTACCCGGGCGCCGAGCACGGCTTCAACTGCACGGACCGCGCGTCGTACAACCAGCGCG
CCGCAGCGCTCGCGCACGGCCGCACGCTGACCTTCCTCGCCGAGCATCTCTAA

Upstream 100 bases:

>100_bases
GCCCGCGTGCACTGCAGCACCGAACGATTGGTGGCACACTAGGCCGCCCTCTTTTCACCCCGGGGCGCCTCGCGCACCCC
GCACGCCAGGAGTTCAGAAC

Downstream 100 bases:

>100_bases
AAAGGCCCGGCCGCGGGCCCGCCACGCCGGGCTCGCGTTATGCTCGCACCATCGCCACGAGTCACAACGGGGGTGACCGC
GATGCACTCGGACTATCTCG

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTAQWIDIPTGNDSFGGYLALPKRGKGPAVIIIQEIFGVNAHIRAVADQYAADGFVALAPDVFWRTQPRVELTYEGADRD
KGIELMKKTDVGLAVADIGAAADALRARPEVAGKLAAIGYCFGGQLAYRAAATGKLDAAVSYYGGGIQNALDLAGQVTQP
ILFHYAENDHGIPLTAVDQVKAAFAGHGDASFHVYPGAEHGFNCTDRASYNQRAAALAHGRTLTFLAEHL

Sequences:

>Translated_230_residues
MTAQWIDIPTGNDSFGGYLALPKRGKGPAVIIIQEIFGVNAHIRAVADQYAADGFVALAPDVFWRTQPRVELTYEGADRD
KGIELMKKTDVGLAVADIGAAADALRARPEVAGKLAAIGYCFGGQLAYRAAATGKLDAAVSYYGGGIQNALDLAGQVTQP
ILFHYAENDHGIPLTAVDQVKAAFAGHGDASFHVYPGAEHGFNCTDRASYNQRAAALAHGRTLTFLAEHL
>Mature_229_residues
TAQWIDIPTGNDSFGGYLALPKRGKGPAVIIIQEIFGVNAHIRAVADQYAADGFVALAPDVFWRTQPRVELTYEGADRDK
GIELMKKTDVGLAVADIGAAADALRARPEVAGKLAAIGYCFGGQLAYRAAATGKLDAAVSYYGGGIQNALDLAGQVTQPI
LFHYAENDHGIPLTAVDQVKAAFAGHGDASFHVYPGAEHGFNCTDRASYNQRAAALAHGRTLTFLAEHL

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=242, Percent_Identity=30.1652892561983, Blast_Score=100, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24343; Mature: 24211

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQWIDIPTGNDSFGGYLALPKRGKGPAVIIIQEIFGVNAHIRAVADQYAADGFVALAP
CCCEEEECCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
DVFWRTQPRVELTYEGADRDKGIELMKKTDVGLAVADIGAAADALRARPEVAGKLAAIGY
CHHCCCCCCEEEEECCCCCCCCHHEEECCCCCEEEEHHHHHHHHHHCCHHHHHHHEEEEE
CFGGQLAYRAAATGKLDAAVSYYGGGIQNALDLAGQVTQPILFHYAENDHGIPLTAVDQV
EECCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHH
KAAFAGHGDASFHVYPGAEHGFNCTDRASYNQRAAALAHGRTLTFLAEHL
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TAQWIDIPTGNDSFGGYLALPKRGKGPAVIIIQEIFGVNAHIRAVADQYAADGFVALAP
CCEEEECCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
DVFWRTQPRVELTYEGADRDKGIELMKKTDVGLAVADIGAAADALRARPEVAGKLAAIGY
CHHCCCCCCEEEEECCCCCCCCHHEEECCCCCEEEEHHHHHHHHHHCCHHHHHHHEEEEE
CFGGQLAYRAAATGKLDAAVSYYGGGIQNALDLAGQVTQPILFHYAENDHGIPLTAVDQV
EECCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHH
KAAFAGHGDASFHVYPGAEHGFNCTDRASYNQRAAALAHGRTLTFLAEHL
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]