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The map label for this gene is nylA [H]

Identifier: 116688299

GI number: 116688299

Start: 316801

End: 318279

Strand: Reverse

Name: nylA [H]

Synonym: Bcen2424_0275

Alternate gene names: 116688299

Gene position: 318279-316801 (Counterclockwise)

Preceding gene: 116688300

Following gene: 116688274

Centisome position: 9.14

GC content: 72.21

Gene sequence:

>1479_bases
ATGCACTCGGACTATCTCGCGCATGACGCAATCGGCCTCGCCGCGCTCGTGCGCGACCGCCAGGCCAGCCCGCGCGAACT
GCTCGACGCCGCGATCGGCCAGGCCGAAGCGGTCAACGGCGCGATCAACGCGATCGTGCTGCAGGATTACGAAGCCGCGC
GCGAACGTGCGGCGGGCGCGCCCGAACCGGGCGCCGCTACGCCGTTCGCGGGCGTGCCGTATCTCGTCAAGGATCTCGGC
GCGGCGGTCGCCGGGCTGCCGCTGTCGATGGGCAGCCGGCACTACCGCTACTTCGTGCCGGCCGACGATTCGCCGGTGAT
CGCGCGCAGCCGCGCGGCGGGCCTGAACGTATTCGGCAAGACCAACACGTCCGAGATCGGCCAGATGCCGTACACCGAAC
CGGCACTGTTCGGCGCGTGCCGCAACCCGTGGAATCTCGATCACACGCCAGGCGGCTCGAGCGGCGGCGCGGCGGCGGTC
GTCGCGGCCGGCATCGTGCCGCTCGCGCATGCGTCCGACGGCGGCGGCTCGATCCGCATTCCGGCGTCGTGCTGCGGGCT
GTTCGGCCTGAAGCCGAGCCGCAACCCGGTGCTGGTCGACCTGCCGTCGAACGGCGAACTCGTCGTCCAGCACGCGGTGT
CGCGCAGCGTGCGCGACAGTGCGCTGCTGCTCGACGTGACGACCGGCCAGACGCTGCCGCCCGGCGCGCCCGGCACCTTC
CTCGGCGCGCTCGATACGCCGCCCGGCCCGCTGCGGATCGGCCTCGTCGTCGATCCGATGCTCGCGCCCGCGCTCGCCGA
CGACACGCGCGCGGCGCTCGACGACGCGGCCGCGCTGCTCGAATCGCTCGGCCATCACGTCGAGCCGGCGACGCTGCAGA
TCGACTATGCGCGCGCGGCCGAAACCTTCCTCACGCTATGGGCGACGATCGCCGAAGAAATGGTGCTCGGCGCGCGCGAA
CTGACCGGCCGCACGCCGCGGCGCAGCGAATTCGAGGCCGCGACCTGGGCGATGGCCGTAGTCGGCAGGCGGCTCGCGCG
CACCCGCCTGCCCGACGTGCTCGAATGGCAGCGCCAGCTCACCGTGCAGGTCGCCGGTCTCGTGTCGCGTTACGACGCGA
TCCTGTGCGCATCGCTCGCGGGGCCGCCGGTGAAGATCGGCGAACTGCAGCCGACGCCGTTCGAATCCGCGCAGATGAAG
CTGGTCGCCGCGCTGCCGGTGAAGCCGCTGCTGAAGGAAATGCTCGCGAAGTCGTCGGAGAAGGCGTTCGCGTGGGCCGG
CTGCACCGAGCTGTTCAACCTGACCGGCCAGCCGGCGATGTCGGTGCCGCTGTACTGGAACGCGCGCGGGCTGCCGATCG
GCGTGCAGTTCGCCGCGCGCCACGCGGACGAAGCGTTGCTGCTCCGGCTCGCGCGGCAGCTCGAACAGGCGCGGCCGTGG
TTCGACCGCCGCCCGCCGCTGATGCAGGCGCAGCGCTGA

Upstream 100 bases:

>100_bases
CCTCGCCGAGCATCTCTAAAAAGGCCCGGCCGCGGGCCCGCCACGCCGGGCTCGCGTTATGCTCGCACCATCGCCACGAG
TCACAACGGGGGTGACCGCG

Downstream 100 bases:

>100_bases
TTCAGCGGCTTCCGCCACGCGGCATGCGCGGCGCTTCACGATAAAAAAGCCCCGCCGGCGCAAACCGGCGGGGCTTTCAC
GCATGCAACCGCGTGACGGC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MHSDYLAHDAIGLAALVRDRQASPRELLDAAIGQAEAVNGAINAIVLQDYEAARERAAGAPEPGAATPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPALFGACRNPWNLDHTPGGSSGGAAAV
VAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVSRSVRDSALLLDVTTGQTLPPGAPGTF
LGALDTPPGPLRIGLVVDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQIDYARAAETFLTLWATIAEEMVLGARE
LTGRTPRRSEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFESAQMK
LVAALPVKPLLKEMLAKSSEKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADEALLLRLARQLEQARPW
FDRRPPLMQAQR

Sequences:

>Translated_492_residues
MHSDYLAHDAIGLAALVRDRQASPRELLDAAIGQAEAVNGAINAIVLQDYEAARERAAGAPEPGAATPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPALFGACRNPWNLDHTPGGSSGGAAAV
VAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVSRSVRDSALLLDVTTGQTLPPGAPGTF
LGALDTPPGPLRIGLVVDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQIDYARAAETFLTLWATIAEEMVLGARE
LTGRTPRRSEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFESAQMK
LVAALPVKPLLKEMLAKSSEKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADEALLLRLARQLEQARPW
FDRRPPLMQAQR
>Mature_492_residues
MHSDYLAHDAIGLAALVRDRQASPRELLDAAIGQAEAVNGAINAIVLQDYEAARERAAGAPEPGAATPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPALFGACRNPWNLDHTPGGSSGGAAAV
VAAGIVPLAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVSRSVRDSALLLDVTTGQTLPPGAPGTF
LGALDTPPGPLRIGLVVDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQIDYARAAETFLTLWATIAEEMVLGARE
LTGRTPRRSEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFESAQMK
LVAALPVKPLLKEMLAKSSEKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADEALLLRLARQLEQARPW
FDRRPPLMQAQR

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=206, Percent_Identity=33.9805825242718, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI222831590, Length=530, Percent_Identity=23.3962264150943, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=24.5544554455446, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=303, Percent_Identity=29.3729372937294, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=473, Percent_Identity=24.9471458773784, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=316, Percent_Identity=26.2658227848101, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=316, Percent_Identity=26.2658227848101, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=171, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550774, Length=504, Percent_Identity=25.7936507936508, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=504, Percent_Identity=25.7936507936508, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648437, Length=504, Percent_Identity=25.7936507936508, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=24.4094488188976, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356731, Length=202, Percent_Identity=31.1881188118812, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=25.0585480093677, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=25.0585480093677, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=28.714859437751, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652985, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922090, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652981, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652983, Length=200, Percent_Identity=32, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161078093, Length=234, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52077; Mature: 52077

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDYLAHDAIGLAALVRDRQASPRELLDAAIGQAEAVNGAINAIVLQDYEAARERAAGA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCCEEEEEECCHHHHHHHCCCC
PEPGAATPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEECC
TNTSEIGQMPYTEPALFGACRNPWNLDHTPGGSSGGAAAVVAAGIVPLAHASDGGGSIRI
CCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEE
PASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVSRSVRDSALLLDVTTGQTLPPGAPGTF
CHHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCE
LGALDTPPGPLRIGLVVDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQIDYARAA
EECCCCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
ETFLTLWATIAEEMVLGARELTGRTPRRSEFEAATWAMAVVGRRLARTRLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFESAQMKLVAALPVKPLLKEMLAKSSE
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHEEEEEECCHHHHHHHHHHCCCC
KAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADEALLLRLARQLEQARPW
CEEEHHHHHHHHCCCCCCCEECEEEECCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHCCC
FDRRPPLMQAQR
CCCCCCCCCCCC
>Mature Secondary Structure
MHSDYLAHDAIGLAALVRDRQASPRELLDAAIGQAEAVNGAINAIVLQDYEAARERAAGA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCCEEEEEECCHHHHHHHCCCC
PEPGAATPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEECC
TNTSEIGQMPYTEPALFGACRNPWNLDHTPGGSSGGAAAVVAAGIVPLAHASDGGGSIRI
CCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEE
PASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVSRSVRDSALLLDVTTGQTLPPGAPGTF
CHHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCE
LGALDTPPGPLRIGLVVDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQIDYARAA
EECCCCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
ETFLTLWATIAEEMVLGARELTGRTPRRSEFEAATWAMAVVGRRLARTRLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFESAQMKLVAALPVKPLLKEMLAKSSE
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHEEEEEECCHHHHHHHHHHCCCC
KAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADEALLLRLARQLEQARPW
CEEEHHHHHHHHCCCCCCCEECEEEECCCCCCEEHHHHHHHCHHHHHHHHHHHHHHHCCC
FDRRPPLMQAQR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]