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The map label for this gene is nreB [H]

Identifier: 116626888

GI number: 116626888

Start: 9865917

End: 9867275

Strand: Direct

Name: nreB [H]

Synonym: Acid_7865

Alternate gene names: 116626888

Gene position: 9865917-9867275 (Clockwise)

Preceding gene: 116626887

Following gene: 116626889

Centisome position: 99.0

GC content: 65.12

Gene sequence:

>1359_bases
GTGGCTTGGAGCAAGCGCGCCGCTCTGCGGGCTGGATACGGCGCTGTCCTGGCCGTCCTCGTCCTCTCCGCCGTTGAGGC
TTACAGCATTCAGGTCGGCGTCTCCCAGCAACATCTCGAAATCTACCGGCGCTTCGTGCAGCAGGACGAAGCGTTGAGCA
CGCTGCGGCGCAACCTCTGGCTGGCCGGCAATCGCGTGCGCGATTTCTTTATCAATCCGACTGAACTCTCCGGCGACGAA
CTGAGCGTCCATTTGCGCGCGCTCAAGGAGGAAGACGAAGCTGTTCTCCGGGTGCTGGAGCGCTGGCCGCAACACCGGGC
AGTGGTCCCGCAATTGCGCGCGAGCCTGGACGAATTCTGGTCGGTGGTAGAGCCGCTGCCCCGGACGATGATGCACGCCT
CAAGGCAAGAGGACATGGCGTTTCTGGAGCGCGAGGTGGTGCCGCGGCGCGGCGAATTGTACAATGCTCTGCGAGCGCTG
ACCTTGGCCGACCAGCGCGCCCTGCAGGATAGCGAAGGCGAATTCGCGGGCACACGGCGGCGCGCCGCCGGACGCCTGAT
GCTGATGCTCGGAGTAGGATTGCTCCTCAGCTTTCTGGTCGCCCGCCTGAGCTTGCGCCACGCCGAAAACCTGGAGCGCG
AGGCCGACCGCCATTTCGCCGCCGTCGAACAGACTCGTGCCGAGTTACAACAGCTCTCCGCGCGTCTCCTCGAGATCGAA
GAGGAAGGCCGGCGCAAGCTCTCACGCGAGTTACACGACGAAATCGGCCAGACCCTGGCGCTGCTGCAGATCGAAATCTC
GCGCGCGCAGGTGGCCCGCGCCGGCACGCCCGCGGCCCTGCGCGAGCATCTGGATCGCGCGCGGGAGCTTGCCGAGCGCA
CTGTGCAAACCGTCCGCAACATCTCCGGCCTGCTCCGTCCCGCGCTGCTCGACGACCTGGGCCTGGTGCCCGCACTGCAA
TTTCAGCTTGAGGATTTCCTGCGTCGCAGCGGCATCGTCTGCGAATTTGTCGAACAAGGCGTCGCCGACCATTTGCCCGA
TCCCGTCAAGACCTGTGTCTACCGCGTCGTCCAGGAAGCGCTGCACAATTGCGAAAAACACTCCGGCGCGCGCAAGGTGC
GTGTTTCCGTGCGGCAGTTCCCCGAAGCCCTGGTGGCGGAGATCGAAGACGATGGCGTGGGATTCCAAATGGGCGGCCGC
CGCGCGTCTGAACGCAACATGGGCCTTGGCCTTCTGGGCATGCGCGAACGCGCCGGAAACGTAGGCGGCTCGCTGGTCAT
CGATTCCGCTCCCCGTCACGGAACCCGGGTTGCTCTCCGCATTCCGCTGGCGCCTCTGGCGATCGAGGTTCAAACGTGA

Upstream 100 bases:

>100_bases
AGGAATAGTCCAATCGTACTGCTGCCGCGGGCGCATTCCCATACGGCAAAGATTGTATTCTCTCCCGGTGCGGGGGCAAC
GATAAAATGATGGGATGGCA

Downstream 100 bases:

>100_bases
TGGCGGTCCGTATTCTGCTCGCCGACGACCACACCATGATGCGGCAGGGGCTGCGCCACATTTTGGAGAGCCGGCCGGAT
TTCGATATCGTCGCCGAAGC

Product: histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAWSKRAALRAGYGAVLAVLVLSAVEAYSIQVGVSQQHLEIYRRFVQQDEALSTLRRNLWLAGNRVRDFFINPTELSGDE
LSVHLRALKEEDEAVLRVLERWPQHRAVVPQLRASLDEFWSVVEPLPRTMMHASRQEDMAFLEREVVPRRGELYNALRAL
TLADQRALQDSEGEFAGTRRRAAGRLMLMLGVGLLLSFLVARLSLRHAENLEREADRHFAAVEQTRAELQQLSARLLEIE
EEGRRKLSRELHDEIGQTLALLQIEISRAQVARAGTPAALREHLDRARELAERTVQTVRNISGLLRPALLDDLGLVPALQ
FQLEDFLRRSGIVCEFVEQGVADHLPDPVKTCVYRVVQEALHNCEKHSGARKVRVSVRQFPEALVAEIEDDGVGFQMGGR
RASERNMGLGLLGMRERAGNVGGSLVIDSAPRHGTRVALRIPLAPLAIEVQT

Sequences:

>Translated_452_residues
MAWSKRAALRAGYGAVLAVLVLSAVEAYSIQVGVSQQHLEIYRRFVQQDEALSTLRRNLWLAGNRVRDFFINPTELSGDE
LSVHLRALKEEDEAVLRVLERWPQHRAVVPQLRASLDEFWSVVEPLPRTMMHASRQEDMAFLEREVVPRRGELYNALRAL
TLADQRALQDSEGEFAGTRRRAAGRLMLMLGVGLLLSFLVARLSLRHAENLEREADRHFAAVEQTRAELQQLSARLLEIE
EEGRRKLSRELHDEIGQTLALLQIEISRAQVARAGTPAALREHLDRARELAERTVQTVRNISGLLRPALLDDLGLVPALQ
FQLEDFLRRSGIVCEFVEQGVADHLPDPVKTCVYRVVQEALHNCEKHSGARKVRVSVRQFPEALVAEIEDDGVGFQMGGR
RASERNMGLGLLGMRERAGNVGGSLVIDSAPRHGTRVALRIPLAPLAIEVQT
>Mature_451_residues
AWSKRAALRAGYGAVLAVLVLSAVEAYSIQVGVSQQHLEIYRRFVQQDEALSTLRRNLWLAGNRVRDFFINPTELSGDEL
SVHLRALKEEDEAVLRVLERWPQHRAVVPQLRASLDEFWSVVEPLPRTMMHASRQEDMAFLEREVVPRRGELYNALRALT
LADQRALQDSEGEFAGTRRRAAGRLMLMLGVGLLLSFLVARLSLRHAENLEREADRHFAAVEQTRAELQQLSARLLEIEE
EGRRKLSRELHDEIGQTLALLQIEISRAQVARAGTPAALREHLDRARELAERTVQTVRNISGLLRPALLDDLGLVPALQF
QLEDFLRRSGIVCEFVEQGVADHLPDPVKTCVYRVVQEALHNCEKHSGARKVRVSVRQFPEALVAEIEDDGVGFQMGGRR
ASERNMGLGLLGMRERAGNVGGSLVIDSAPRHGTRVALRIPLAPLAIEVQT

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=223, Percent_Identity=35.4260089686099, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787474, Length=227, Percent_Identity=28.1938325991189, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50731; Mature: 50600

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWSKRAALRAGYGAVLAVLVLSAVEAYSIQVGVSQQHLEIYRRFVQQDEALSTLRRNLW
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAGNRVRDFFINPTELSGDELSVHLRALKEEDEAVLRVLERWPQHRAVVPQLRASLDEFW
HCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SVVEPLPRTMMHASRQEDMAFLEREVVPRRGELYNALRALTLADQRALQDSEGEFAGTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAAGRLMLMLGVGLLLSFLVARLSLRHAENLEREADRHFAAVEQTRAELQQLSARLLEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGRRKLSRELHDEIGQTLALLQIEISRAQVARAGTPAALREHLDRARELAERTVQTVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ISGLLRPALLDDLGLVPALQFQLEDFLRRSGIVCEFVEQGVADHLPDPVKTCVYRVVQEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHH
LHNCEKHSGARKVRVSVRQFPEALVAEIEDDGVGFQMGGRRASERNMGLGLLGMRERAGN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
VGGSLVIDSAPRHGTRVALRIPLAPLAIEVQT
CCCCEEECCCCCCCCEEEEEECCCCEEEEECC
>Mature Secondary Structure 
AWSKRAALRAGYGAVLAVLVLSAVEAYSIQVGVSQQHLEIYRRFVQQDEALSTLRRNLW
CCCHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAGNRVRDFFINPTELSGDELSVHLRALKEEDEAVLRVLERWPQHRAVVPQLRASLDEFW
HCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SVVEPLPRTMMHASRQEDMAFLEREVVPRRGELYNALRALTLADQRALQDSEGEFAGTRR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAAGRLMLMLGVGLLLSFLVARLSLRHAENLEREADRHFAAVEQTRAELQQLSARLLEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGRRKLSRELHDEIGQTLALLQIEISRAQVARAGTPAALREHLDRARELAERTVQTVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ISGLLRPALLDDLGLVPALQFQLEDFLRRSGIVCEFVEQGVADHLPDPVKTCVYRVVQEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHH
LHNCEKHSGARKVRVSVRQFPEALVAEIEDDGVGFQMGGRRASERNMGLGLLGMRERAGN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
VGGSLVIDSAPRHGTRVALRIPLAPLAIEVQT
CCCCEEECCCCCCCCEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]