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The map label for this gene is rph

Identifier: 116626750

GI number: 116626750

Start: 9698493

End: 9699209

Strand: Direct

Name: rph

Synonym: Acid_7723

Alternate gene names: 116626750

Gene position: 9698493-9699209 (Clockwise)

Preceding gene: 116626746

Following gene: 116626751

Centisome position: 97.32

GC content: 62.2

Gene sequence:

>717_bases
GTGCGAACGGACAATAGAACAGCCGGCCAACTACGACCCGTCCAAATCACGACCGGTTGTCTGATGACAGCGGAGGGCTC
CGCGTTGATCAAGGTGGGGAACACGCACGTGCTCTGCGCCGCGACCATCGAAGATACCGTACCGCCCTTTCTGCGCAACA
CCGGCAAGGGATGGGTGACGGCGGAGTATTCGATGCTGCCGCGCGCCACAGCAAAACGCACACCGCGCGAAGTGACGAAG
GGACGCCCCTCGGGAAGGACCCACGAGATCCAGCGATTGATCGGACGCTCCATGCGCTCGGCGGTAGATATGAGCTCGTT
CGGCGAGCGCACGCTGATTATCGATTGCGATGTGATCCAGGCCGATGGGGGAACCCGCACGGCATCGATTACGGGCGCCT
TCGTGGCGATGGCGCTGGCCTTCCGGCAACTGATTGAATACCGCGCGCTGAAGGCCAACCCGATCCGCGATTATGTGGCG
GCCACCAGCGTGGGACTGGTCGGCGGAATCCCGATGCTCGACCTTTGCTACGAGGAAGACTCGCAGGCCGATGTGGACAT
GAATGTGGTGATGACGGGAGGCGGCAAGTTTGTCGAAGTCCAGGCCACCGCGGAGCATAGCGCGTTCGACGATGCGCAGA
TGGCCGAGCTGACCGAGCTCGCGCGCACGGGAATCGCGCAACTGGTCCGCATGCAGAAGGAGATCATCGAAGGTTGA

Upstream 100 bases:

>100_bases
GGCAGGAAGAATCCGCGCCCGCAGCAGGCCGCCGGCTGCGTCATCCTGATCCTGTTGATGATGGTTGGAGTGATGGTCTT
CCTTTATCTGGTGATGAAAA

Downstream 100 bases:

>100_bases
CGACGCTGTATTGCGCTACTGGGAACGCCGGCAAGCTGCGCGAATTTCGGATGGCGGCCGATCACGCACCATTCGATATC
GAGCTGCTTCCGAATTACAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRTDNRTAGQLRPVQITTGCLMTAEGSALIKVGNTHVLCAATIEDTVPPFLRNTGKGWVTAEYSMLPRATAKRTPREVTK
GRPSGRTHEIQRLIGRSMRSAVDMSSFGERTLIIDCDVIQADGGTRTASITGAFVAMALAFRQLIEYRALKANPIRDYVA
ATSVGLVGGIPMLDLCYEEDSQADVDMNVVMTGGGKFVEVQATAEHSAFDDAQMAELTELARTGIAQLVRMQKEIIEG

Sequences:

>Translated_238_residues
MRTDNRTAGQLRPVQITTGCLMTAEGSALIKVGNTHVLCAATIEDTVPPFLRNTGKGWVTAEYSMLPRATAKRTPREVTK
GRPSGRTHEIQRLIGRSMRSAVDMSSFGERTLIIDCDVIQADGGTRTASITGAFVAMALAFRQLIEYRALKANPIRDYVA
ATSVGLVGGIPMLDLCYEEDSQADVDMNVVMTGGGKFVEVQATAEHSAFDDAQMAELTELARTGIAQLVRMQKEIIEG
>Mature_238_residues
MRTDNRTAGQLRPVQITTGCLMTAEGSALIKVGNTHVLCAATIEDTVPPFLRNTGKGWVTAEYSMLPRATAKRTPREVTK
GRPSGRTHEIQRLIGRSMRSAVDMSSFGERTLIIDCDVIQADGGTRTASITGAFVAMALAFRQLIEYRALKANPIRDYVA
ATSVGLVGGIPMLDLCYEEDSQADVDMNVVMTGGGKFVEVQATAEHSAFDDAQMAELTELARTGIAQLVRMQKEIIEG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Homo sapiens, GI9506689, Length=190, Percent_Identity=30.5263157894737, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=55.5023923444976, Blast_Score=234, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SOLUE (Q01NZ9)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_828906.1
- ProteinModelPortal:   Q01NZ9
- SMR:   Q01NZ9
- STRING:   Q01NZ9
- GeneID:   4431339
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_7723
- NMPDR:   fig|234267.9.peg.7326
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q01NZ9
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25740; Mature: 25740

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDNRTAGQLRPVQITTGCLMTAEGSALIKVGNTHVLCAATIEDTVPPFLRNTGKGWVT
CCCCCCCCCCEEEEEEECCEEEEECCCEEEEECCCEEEEEECCHHCCCHHHHCCCCCEEE
AEYSMLPRATAKRTPREVTKGRPSGRTHEIQRLIGRSMRSAVDMSSFGERTLIIDCDVIQ
EEHHHCCCHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFVAMALAFRQLIEYRALKANPIRDYVAATSVGLVGGIPMLDLCYEED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHEECCC
SQADVDMNVVMTGGGKFVEVQATAEHSAFDDAQMAELTELARTGIAQLVRMQKEIIEG
CCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTDNRTAGQLRPVQITTGCLMTAEGSALIKVGNTHVLCAATIEDTVPPFLRNTGKGWVT
CCCCCCCCCCEEEEEEECCEEEEECCCEEEEECCCEEEEEECCHHCCCHHHHCCCCCEEE
AEYSMLPRATAKRTPREVTKGRPSGRTHEIQRLIGRSMRSAVDMSSFGERTLIIDCDVIQ
EEHHHCCCHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFVAMALAFRQLIEYRALKANPIRDYVAATSVGLVGGIPMLDLCYEED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHEECCC
SQADVDMNVVMTGGGKFVEVQATAEHSAFDDAQMAELTELARTGIAQLVRMQKEIIEG
CCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA