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The map label for this gene is gutB [H]

Identifier: 116626572

GI number: 116626572

Start: 9442799

End: 9443830

Strand: Direct

Name: gutB [H]

Synonym: Acid_7535

Alternate gene names: 116626572

Gene position: 9442799-9443830 (Clockwise)

Preceding gene: 116626567

Following gene: 116626573

Centisome position: 94.75

GC content: 66.47

Gene sequence:

>1032_bases
ATGAACGCACTTCTTCTCACGCAATACATGCACCTGGAAATGGTGGAGATGCCGGTCCCGGCGATTGGAGCCGACGACGT
GCTGGTCCGCGTCCGCGCCTGCGGCATCTGCGGCAGCGACGTCCACGGGCTGGACGGCAAGACGGGCCGGCGCATTCCGC
CCCTGGTAATGGGCCACGAGGCCGCGGGCGAAGTTGTGGAAACCGGCGCCAACGTGACCGACCTGCGCCCGGGAGACCGG
GTCACGTTCGATTCCACCGTGTATTGCGGGCGCTGCTTCCACTGCACGCGAGGCGAAGTGAACCTCTGCGATAACCGCGA
AGTGCTGGGCGTTTCGCCCGGACCCTACCGCCGCCACGGGGCCTTCGCGGAGTACGTCTCGGTGCCGCGGCGGATCATGT
ACCGGCTGCCGGATAGCTTAAGTTACGAGCAGGCGGCGCTGATCGAGGCCGTCTCCGTCGCAGTGCACGCCGTAAACCTG
ACGCCGGTGCGGCTGGGCGATTCCGCGGTGGTAGTCGGCAGCGGCATGATCGGCTTGCTGGCGATGCAGGCGGCGAAGCA
TGCGGGATGCACGCGCGTGATCGCGGTGGATCCCGACGAAGGGCGATTGAGACTCGCGATTGGTGCGGGTGCGACCGATG
CGATCAATCCCAAATCCACCGACGCCGCGGCGGCAATCCGCGAGTTGACCGAAGGCCGCGGCGCTGATCTCGCGATCGAG
TGCGTGGGCGCCACCGACCCGATCCGGACGGCGATCGCCGGAGTGCGGAAGGGCGGCGCGGTGACTCTGGTGGGGAACGT
GGCGCCGGAAATCGAGTTCCCGCTGCAATCCGTGGTGACGCGGCAGATCCGCTTGCAGGGATCGTGCGCCTCCAACGGCG
AATATCCGGCGTGCATCGACATGATGGCGCGCGGCGCGATCCGGGTGGATGAGTTGATCACCGCTGTGGCGCCGCTCGAA
GAAGGCCCTTCGTGGTTCGACCGTCTCTACCGTCACGAGCCGCATTTGATGAAGGTGATCCTGCGTCCGTAA

Upstream 100 bases:

>100_bases
CGGGCTCCTGGGGATCTGGCACCTGCAACCTCCGGGGTTATGACGCCAATTTGGCAGGCCCGGTTCCCGATTTCGATCGT
GCGATGATGATCTCGTGGCC

Downstream 100 bases:

>100_bases
AGGTGTGCATGCCTCAAGCGGCTTGCCGCACTGTACACTGCTTTTGTTTGCTCGCAGCCCGCAAAGATTTGAGAGGGCCT
GGTTTTGTGGGGCAGGCATT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNALLLTQYMHLEMVEMPVPAIGADDVLVRVRACGICGSDVHGLDGKTGRRIPPLVMGHEAAGEVVETGANVTDLRPGDR
VTFDSTVYCGRCFHCTRGEVNLCDNREVLGVSPGPYRRHGAFAEYVSVPRRIMYRLPDSLSYEQAALIEAVSVAVHAVNL
TPVRLGDSAVVVGSGMIGLLAMQAAKHAGCTRVIAVDPDEGRLRLAIGAGATDAINPKSTDAAAAIRELTEGRGADLAIE
CVGATDPIRTAIAGVRKGGAVTLVGNVAPEIEFPLQSVVTRQIRLQGSCASNGEYPACIDMMARGAIRVDELITAVAPLE
EGPSWFDRLYRHEPHLMKVILRP

Sequences:

>Translated_343_residues
MNALLLTQYMHLEMVEMPVPAIGADDVLVRVRACGICGSDVHGLDGKTGRRIPPLVMGHEAAGEVVETGANVTDLRPGDR
VTFDSTVYCGRCFHCTRGEVNLCDNREVLGVSPGPYRRHGAFAEYVSVPRRIMYRLPDSLSYEQAALIEAVSVAVHAVNL
TPVRLGDSAVVVGSGMIGLLAMQAAKHAGCTRVIAVDPDEGRLRLAIGAGATDAINPKSTDAAAAIRELTEGRGADLAIE
CVGATDPIRTAIAGVRKGGAVTLVGNVAPEIEFPLQSVVTRQIRLQGSCASNGEYPACIDMMARGAIRVDELITAVAPLE
EGPSWFDRLYRHEPHLMKVILRP
>Mature_343_residues
MNALLLTQYMHLEMVEMPVPAIGADDVLVRVRACGICGSDVHGLDGKTGRRIPPLVMGHEAAGEVVETGANVTDLRPGDR
VTFDSTVYCGRCFHCTRGEVNLCDNREVLGVSPGPYRRHGAFAEYVSVPRRIMYRLPDSLSYEQAALIEAVSVAVHAVNL
TPVRLGDSAVVVGSGMIGLLAMQAAKHAGCTRVIAVDPDEGRLRLAIGAGATDAINPKSTDAAAAIRELTEGRGADLAIE
CVGATDPIRTAIAGVRKGGAVTLVGNVAPEIEFPLQSVVTRQIRLQGSCASNGEYPACIDMMARGAIRVDELITAVAPLE
EGPSWFDRLYRHEPHLMKVILRP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=325, Percent_Identity=32.3076923076923, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4501929, Length=352, Percent_Identity=28.9772727272727, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4501933, Length=354, Percent_Identity=27.9661016949153, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI34577061, Length=348, Percent_Identity=28.448275862069, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI71565152, Length=355, Percent_Identity=26.4788732394366, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI262073058, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI71743840, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI71565154, Length=347, Percent_Identity=27.6657060518732, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI156523966, Length=301, Percent_Identity=26.9102990033223, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4501939, Length=301, Percent_Identity=26.9102990033223, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788073, Length=339, Percent_Identity=32.7433628318584, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790045, Length=279, Percent_Identity=33.6917562724014, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787863, Length=342, Percent_Identity=28.3625730994152, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI226510992, Length=343, Percent_Identity=28.8629737609329, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788075, Length=342, Percent_Identity=28.0701754385965, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1786825, Length=388, Percent_Identity=28.6082474226804, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788407, Length=259, Percent_Identity=31.2741312741313, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786552, Length=356, Percent_Identity=30.8988764044944, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082125, Length=292, Percent_Identity=32.1917808219178, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081918, Length=291, Percent_Identity=31.2714776632302, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790718, Length=300, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787572, Length=381, Percent_Identity=26.509186351706, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082401, Length=313, Percent_Identity=26.1980830670927, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786518, Length=252, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=337, Percent_Identity=35.3115727002967, Blast_Score=193, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17562878, Length=323, Percent_Identity=34.3653250773994, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25146526, Length=354, Percent_Identity=27.683615819209, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71997431, Length=354, Percent_Identity=27.1186440677966, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17565904, Length=334, Percent_Identity=28.1437125748503, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17562584, Length=339, Percent_Identity=31.2684365781711, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71988145, Length=315, Percent_Identity=28.8888888888889, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562582, Length=325, Percent_Identity=29.2307692307692, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=350, Percent_Identity=32.2857142857143, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322619, Length=350, Percent_Identity=32.2857142857143, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323099, Length=349, Percent_Identity=30.0859598853868, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320033, Length=361, Percent_Identity=29.3628808864266, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319257, Length=357, Percent_Identity=28.0112044817927, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319258, Length=366, Percent_Identity=28.9617486338798, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324486, Length=325, Percent_Identity=27.3846153846154, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=319, Percent_Identity=28.2131661442006, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=304, Percent_Identity=27.3026315789474, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319621, Length=329, Percent_Identity=27.355623100304, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319949, Length=138, Percent_Identity=31.1594202898551, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323980, Length=125, Percent_Identity=32, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137530, Length=332, Percent_Identity=34.0361445783133, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17737897, Length=347, Percent_Identity=34.2939481268012, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17737895, Length=350, Percent_Identity=28.8571428571429, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI221457811, Length=350, Percent_Identity=26.5714285714286, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45550770, Length=350, Percent_Identity=26.5714285714286, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45551930, Length=350, Percent_Identity=26.5714285714286, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36539; Mature: 36539

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLLTQYMHLEMVEMPVPAIGADDVLVRVRACGICGSDVHGLDGKTGRRIPPLVMGHE
CCCEEEEHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCH
AAGEVVETGANVTDLRPGDRVTFDSTVYCGRCFHCTRGEVNLCDNREVLGVSPGPYRRHG
HHHHHHHCCCCEEECCCCCCEEECCEEEECEEEEECCCCEEECCCCEEEECCCCCCHHCC
AFAEYVSVPRRIMYRLPDSLSYEQAALIEAVSVAVHAVNLTPVRLGDSAVVVGSGMIGLL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECEEEEECCCEEEECCCHHHHH
AMQAAKHAGCTRVIAVDPDEGRLRLAIGAGATDAINPKSTDAAAAIRELTEGRGADLAIE
HHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
CVGATDPIRTAIAGVRKGGAVTLVGNVAPEIEFPLQSVVTRQIRLQGSCASNGEYPACID
EECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCHHHH
MMARGAIRVDELITAVAPLEEGPSWFDRLYRHEPHLMKVILRP
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MNALLLTQYMHLEMVEMPVPAIGADDVLVRVRACGICGSDVHGLDGKTGRRIPPLVMGHE
CCCEEEEHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCH
AAGEVVETGANVTDLRPGDRVTFDSTVYCGRCFHCTRGEVNLCDNREVLGVSPGPYRRHG
HHHHHHHCCCCEEECCCCCCEEECCEEEECEEEEECCCCEEECCCCEEEECCCCCCHHCC
AFAEYVSVPRRIMYRLPDSLSYEQAALIEAVSVAVHAVNLTPVRLGDSAVVVGSGMIGLL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEECEEEEECCCEEEECCCHHHHH
AMQAAKHAGCTRVIAVDPDEGRLRLAIGAGATDAINPKSTDAAAAIRELTEGRGADLAIE
HHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
CVGATDPIRTAIAGVRKGGAVTLVGNVAPEIEFPLQSVVTRQIRLQGSCASNGEYPACID
EECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCHHHH
MMARGAIRVDELITAVAPLEEGPSWFDRLYRHEPHLMKVILRP
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]