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The map label for this gene is dgoD1 [H]

Identifier: 116625827

GI number: 116625827

Start: 8501616

End: 8502944

Strand: Direct

Name: dgoD1 [H]

Synonym: Acid_6778

Alternate gene names: 116625827

Gene position: 8501616-8502944 (Clockwise)

Preceding gene: 116625826

Following gene: 116625828

Centisome position: 85.31

GC content: 63.81

Gene sequence:

>1329_bases
ATGAAGCGCCGAAACCTTTTAGGAATGTTCGCCGCCGCAGGCGCGATGGGCGCGAACCGCCGGCGGCTGGTGGACTGCGG
CTGTGCCATGCAAGTGCAGCACTCGAAGGCCATGCCGCCGGGTTCGGATGTTTTCAAGGACGTCGGTTCGAATCTCCGCA
TCACCAACATGCGCGTGTTCGGCGTGACGCTTGACGAGCGCATCGCGCAGGCGGACCGGCCCTATGTGTTCGTGAAGATC
GAAACCAACCAGGGGGTTGTCGGCTGGGGCGAAGCCACGCTCGAAGGCAAGGCTTCCGCGGCCATGGCCTGCGTAATGGA
CCTGAAGGATTACATCATCGGCAGCGACCCGATGCAGGTGGAGCACCTCTGGCAGCTCATGTACGTCGGCAGCTTCTATC
GGGGCGGTCCGGTGCTCGGGTCGGCCATCTCGGGTATCGACCAGGCGCTGTGGGATATCCGCGGCAAGGTCATGAACCTG
CCCGTATATGAGATGCTGGGCGGCCCGGTGGATCCGCGCGGCGTCCGCGGCTACTATCACGCCTCGGCCTGGTCTCTCTC
TGAGGCGCGCGAGTTGCGCGACCAGACCAGACAAGCGGGAGTTACTGCGCTCAAATTCCAACTCCCCGACCTGCTCGAGT
GGGTGGAGACGGGCGCCAAGATCAAGCGGGCAGTCCGGCATATGGAGATGCTTCGGGAAGGCCTCGGGCCCGACCTGGAT
TTCGCCGTGGACTTTCATGCGCGCCCAAGCCCCACCGTCGCGGCCATCATCCTCCGCGAGATCGAACCGCTACATCTGCT
TTTCGCCGAAGAAATCTGTCCGCCGGAAAACGTGCGCGCTATGGCCCGCGCAGTCAAGTATTCCACCACGCCCATAGCGA
CAGGCGAACGGCTGATCGCGTCGTACGGCTACGCGGAGCTCATCGACCTGGGTGTGGTGGACATCCTTCAGCCCGATATT
GCGCACGTCGGCGGCGTGACCGCCTTATGGAAGGTGTCGGCCGCCGCCGAGGCATCCGGCATCCGTATGGCTCCTCATGC
GTGCGAGGGCCCAATTGGCGGCATCGCCAGCCTGCACGTGGATGCGGCCGGCCCCAATTTCCTGGCGCAGGAGATCTGCG
GCTTTGTGGATGCGGGCGAAAAGGGCAGAGTGTGGGAAGATCTGATGGGCTTCCCGGCCATGCGTATGGTGAACGGCCGT
TATCCCCTTCCCACGCGACCCGGTCTCGGTATCGATATCAGTGAGGCGGCGATCAAGCGATATCCGTTCCAGGGCACGCG
TCCCTTCCCGCCCGCGTTTCACGAGGACGGCTCGCTCGCTTCGCTCTAG

Upstream 100 bases:

>100_bases
AAGAAGCGGCCGAAAACGGTTTCGCCGCCCTGATGAATCGTCCGGGCCTGGGATTCGAGGTGAACCGCAAGGCTCTCGAC
ACGTACAGTGAGGAGCGTAT

Downstream 100 bases:

>100_bases
CGAGGTCTACGACCTCAAACCGACGAGCATACGGCCGGAGCATTTCGTAAAATCCGAGGATGGCGACTATCCCGGAGTTG
TTGGACGGGCACGTCGCGCT

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD 1 [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKRRNLLGMFAAAGAMGANRRRLVDCGCAMQVQHSKAMPPGSDVFKDVGSNLRITNMRVFGVTLDERIAQADRPYVFVKI
ETNQGVVGWGEATLEGKASAAMACVMDLKDYIIGSDPMQVEHLWQLMYVGSFYRGGPVLGSAISGIDQALWDIRGKVMNL
PVYEMLGGPVDPRGVRGYYHASAWSLSEARELRDQTRQAGVTALKFQLPDLLEWVETGAKIKRAVRHMEMLREGLGPDLD
FAVDFHARPSPTVAAIILREIEPLHLLFAEEICPPENVRAMARAVKYSTTPIATGERLIASYGYAELIDLGVVDILQPDI
AHVGGVTALWKVSAAAEASGIRMAPHACEGPIGGIASLHVDAAGPNFLAQEICGFVDAGEKGRVWEDLMGFPAMRMVNGR
YPLPTRPGLGIDISEAAIKRYPFQGTRPFPPAFHEDGSLASL

Sequences:

>Translated_442_residues
MKRRNLLGMFAAAGAMGANRRRLVDCGCAMQVQHSKAMPPGSDVFKDVGSNLRITNMRVFGVTLDERIAQADRPYVFVKI
ETNQGVVGWGEATLEGKASAAMACVMDLKDYIIGSDPMQVEHLWQLMYVGSFYRGGPVLGSAISGIDQALWDIRGKVMNL
PVYEMLGGPVDPRGVRGYYHASAWSLSEARELRDQTRQAGVTALKFQLPDLLEWVETGAKIKRAVRHMEMLREGLGPDLD
FAVDFHARPSPTVAAIILREIEPLHLLFAEEICPPENVRAMARAVKYSTTPIATGERLIASYGYAELIDLGVVDILQPDI
AHVGGVTALWKVSAAAEASGIRMAPHACEGPIGGIASLHVDAAGPNFLAQEICGFVDAGEKGRVWEDLMGFPAMRMVNGR
YPLPTRPGLGIDISEAAIKRYPFQGTRPFPPAFHEDGSLASL
>Mature_442_residues
MKRRNLLGMFAAAGAMGANRRRLVDCGCAMQVQHSKAMPPGSDVFKDVGSNLRITNMRVFGVTLDERIAQADRPYVFVKI
ETNQGVVGWGEATLEGKASAAMACVMDLKDYIIGSDPMQVEHLWQLMYVGSFYRGGPVLGSAISGIDQALWDIRGKVMNL
PVYEMLGGPVDPRGVRGYYHASAWSLSEARELRDQTRQAGVTALKFQLPDLLEWVETGAKIKRAVRHMEMLREGLGPDLD
FAVDFHARPSPTVAAIILREIEPLHLLFAEEICPPENVRAMARAVKYSTTPIATGERLIASYGYAELIDLGVVDILQPDI
AHVGGVTALWKVSAAAEASGIRMAPHACEGPIGGIASLHVDAAGPNFLAQEICGFVDAGEKGRVWEDLMGFPAMRMVNGR
YPLPTRPGLGIDISEAAIKRYPFQGTRPFPPAFHEDGSLASL

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=368, Percent_Identity=35.5978260869565, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1787864, Length=397, Percent_Identity=29.4710327455919, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI226510960, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 48058; Mature: 48058

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRNLLGMFAAAGAMGANRRRLVDCGCAMQVQHSKAMPPGSDVFKDVGSNLRITNMRVF
CCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEHHCCCCCCCHHHHHHCCCCEEEEEEEEE
GVTLDERIAQADRPYVFVKIETNQGVVGWGEATLEGKASAAMACVMDLKDYIIGSDPMQV
EEEHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
EHLWQLMYVGSFYRGGPVLGSAISGIDQALWDIRGKVMNLPVYEMLGGPVDPRGVRGYYH
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCCEEC
ASAWSLSEARELRDQTRQAGVTALKFQLPDLLEWVETGAKIKRAVRHMEMLREGLGPDLD
CCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FAVDFHARPSPTVAAIILREIEPLHLLFAEEICPPENVRAMARAVKYSTTPIATGERLIA
EEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
SYGYAELIDLGVVDILQPDIAHVGGVTALWKVSAAAEASGIRMAPHACEGPIGGIASLHV
HCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEE
DAAGPNFLAQEICGFVDAGEKGRVWEDLMGFPAMRMVNGRYPLPTRPGLGIDISEAAIKR
ECCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHH
YPFQGTRPFPPAFHEDGSLASL
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRRNLLGMFAAAGAMGANRRRLVDCGCAMQVQHSKAMPPGSDVFKDVGSNLRITNMRVF
CCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEHHCCCCCCCHHHHHHCCCCEEEEEEEEE
GVTLDERIAQADRPYVFVKIETNQGVVGWGEATLEGKASAAMACVMDLKDYIIGSDPMQV
EEEHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
EHLWQLMYVGSFYRGGPVLGSAISGIDQALWDIRGKVMNLPVYEMLGGPVDPRGVRGYYH
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCCEEC
ASAWSLSEARELRDQTRQAGVTALKFQLPDLLEWVETGAKIKRAVRHMEMLREGLGPDLD
CCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FAVDFHARPSPTVAAIILREIEPLHLLFAEEICPPENVRAMARAVKYSTTPIATGERLIA
EEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
SYGYAELIDLGVVDILQPDIAHVGGVTALWKVSAAAEASGIRMAPHACEGPIGGIASLHV
HCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEE
DAAGPNFLAQEICGFVDAGEKGRVWEDLMGFPAMRMVNGRYPLPTRPGLGIDISEAAIKR
ECCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHH
YPFQGTRPFPPAFHEDGSLASL
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA