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The map label for this gene is moaA [H]

Identifier: 116625479

GI number: 116625479

Start: 8045584

End: 8046579

Strand: Reverse

Name: moaA [H]

Synonym: Acid_6425

Alternate gene names: 116625479

Gene position: 8046579-8045584 (Counterclockwise)

Preceding gene: 116625480

Following gene: 116625478

Centisome position: 80.74

GC content: 63.96

Gene sequence:

>996_bases
ATGAACGCCACACCCCTGGTCGATTCCTTTCACCGGGTCCACGACAATCTGCGCATCAGCGTCACCGACCGCTGCAACAT
CCGGTGTTTTTACTGCATGCCCGAAACCGGCGTGACGTTCGTGGAGCGCCGCGAAATACTGGATTTCGAGGAGATCGAAC
GCTTCGTGCGGGTCGCGGCCGGCCTCGGGATTCGCAAGCTGCGCGTCACCGGCGGCGAACCCCTCCTGCGCCGCGACTTG
CCCGTGCTGATCGGGCGCCTCGCCTGCATTCCCGGCATTCAAGACCTGGCCCTGACCACCAACGGCGTGCTGCTGGCCGA
GCAGGCCGAGGCGCTCTACGCCGCGGGCCTGCGCCGTCTCAACGTCCACATCGATACGCTGGATCGCGACCGCTTCTTTC
AGATTACGCGGCGCGACGATCTGGACCGTGTGCTTGCCGGCCTCGACGTCGCGCGCCGCACCGGTTTCAAGATCAAGCTC
AATGCCGTTGCTGTGAAGAACCTCGTGGAACCGGACATCGTGCCCCTGGCGCGCTACGCCCGCGAGCACGGCTTCGAGGT
GCGGTACATCGAGTTTATGCCGCTCGATGCGCAGAATCTTTGGGACCGCGGCAAGGTGCTCCTGGCCGACGACATGATCG
ACCAGCTCTCGCGCGAAATCTCTCCCTTGAGTCCCTTGCCCGACCCCGACCCCCGCGCGCCGGCCACCGAATACGTCTAC
CAGGATGGCGGCGGCACGGTCGGCTTCATCGCGTCGGTAAGCCGCCCCTTCTGCCTGAACTGCAATCGCCTCCGCATCAC
CGCCGACGGCAAACTCCGCTACTGCCTGTTCGCCATCAATGAAGACGACGTGAAGACTCTGATGCGCTCCGGCGCATCCG
ACGAAGCCATCGCCGCCCTGATCCGCCGCAATGTCGCAGCCAAATGGGAAGGCCACGAAATCAACTCCAGTAAATTCGTA
GCCCCTCCCCGCCCCATGTACTCCATTGGCGGCTAA

Upstream 100 bases:

>100_bases
TGCGCGATTGCCTGCGCGACCTGCAACCGCGGGTCGAGCTCTCCGACGACATCATGCGGCGGGCGCTGGTCCCGCTGGAG
CGAATGCTCGCGGTGAAATA

Downstream 100 bases:

>100_bases
AAGCCTCACCCCACCGCCGGTAGCACCTGCTGCGCTTCGACAACTGTGGTGGGGAATGCCCTCTTTCCCACTCTTCTTTG
CGTTACTTTGCGGCTTTGCG

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNATPLVDSFHRVHDNLRISVTDRCNIRCFYCMPETGVTFVERREILDFEEIERFVRVAAGLGIRKLRVTGGEPLLRRDL
PVLIGRLACIPGIQDLALTTNGVLLAEQAEALYAAGLRRLNVHIDTLDRDRFFQITRRDDLDRVLAGLDVARRTGFKIKL
NAVAVKNLVEPDIVPLARYAREHGFEVRYIEFMPLDAQNLWDRGKVLLADDMIDQLSREISPLSPLPDPDPRAPATEYVY
QDGGGTVGFIASVSRPFCLNCNRLRITADGKLRYCLFAINEDDVKTLMRSGASDEAIAALIRRNVAAKWEGHEINSSKFV
APPRPMYSIGG

Sequences:

>Translated_331_residues
MNATPLVDSFHRVHDNLRISVTDRCNIRCFYCMPETGVTFVERREILDFEEIERFVRVAAGLGIRKLRVTGGEPLLRRDL
PVLIGRLACIPGIQDLALTTNGVLLAEQAEALYAAGLRRLNVHIDTLDRDRFFQITRRDDLDRVLAGLDVARRTGFKIKL
NAVAVKNLVEPDIVPLARYAREHGFEVRYIEFMPLDAQNLWDRGKVLLADDMIDQLSREISPLSPLPDPDPRAPATEYVY
QDGGGTVGFIASVSRPFCLNCNRLRITADGKLRYCLFAINEDDVKTLMRSGASDEAIAALIRRNVAAKWEGHEINSSKFV
APPRPMYSIGG
>Mature_331_residues
MNATPLVDSFHRVHDNLRISVTDRCNIRCFYCMPETGVTFVERREILDFEEIERFVRVAAGLGIRKLRVTGGEPLLRRDL
PVLIGRLACIPGIQDLALTTNGVLLAEQAEALYAAGLRRLNVHIDTLDRDRFFQITRRDDLDRVLAGLDVARRTGFKIKL
NAVAVKNLVEPDIVPLARYAREHGFEVRYIEFMPLDAQNLWDRGKVLLADDMIDQLSREISPLSPLPDPDPRAPATEYVY
QDGGGTVGFIASVSRPFCLNCNRLRITADGKLRYCLFAINEDDVKTLMRSGASDEAIAALIRRNVAAKWEGHEINSSKFV
APPRPMYSIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=327, Percent_Identity=37.0030581039755, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI116734668, Length=327, Percent_Identity=37.0030581039755, Blast_Score=214, Evalue=8e-56,
Organism=Escherichia coli, GI1786999, Length=336, Percent_Identity=32.4404761904762, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32566329, Length=330, Percent_Identity=36.969696969697, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17561210, Length=330, Percent_Identity=36.6666666666667, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI32566331, Length=313, Percent_Identity=36.741214057508, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI28574488, Length=331, Percent_Identity=38.6706948640483, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI28574490, Length=310, Percent_Identity=38.7096774193548, Blast_Score=228, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37176; Mature: 37176

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATPLVDSFHRVHDNLRISVTDRCNIRCFYCMPETGVTFVERREILDFEEIERFVRVAA
CCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHH
GLGIRKLRVTGGEPLLRRDLPVLIGRLACIPGIQDLALTTNGVLLAEQAEALYAAGLRRL
CCCEEEEEECCCCCHHHHCHHHHHHHHHHCCCCCHHEEECCCEEEEHHHHHHHHHCCEEE
NVHIDTLDRDRFFQITRRDDLDRVLAGLDVARRTGFKIKLNAVAVKNLVEPDIVPLARYA
EEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHEEHHHHCCCCHHHHHHHH
REHGFEVRYIEFMPLDAQNLWDRGKVLLADDMIDQLSREISPLSPLPDPDPRAPATEYVY
HHCCCEEEEEEEEECCHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
QDGGGTVGFIASVSRPFCLNCNRLRITADGKLRYCLFAINEDDVKTLMRSGASDEAIAAL
ECCCCEEEEEECCCCCEEECCCEEEEEECCCEEEEEEEECCHHHHHHHHCCCCHHHHHHH
IRRNVAAKWEGHEINSSKFVAPPRPMYSIGG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNATPLVDSFHRVHDNLRISVTDRCNIRCFYCMPETGVTFVERREILDFEEIERFVRVAA
CCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHH
GLGIRKLRVTGGEPLLRRDLPVLIGRLACIPGIQDLALTTNGVLLAEQAEALYAAGLRRL
CCCEEEEEECCCCCHHHHCHHHHHHHHHHCCCCCHHEEECCCEEEEHHHHHHHHHCCEEE
NVHIDTLDRDRFFQITRRDDLDRVLAGLDVARRTGFKIKLNAVAVKNLVEPDIVPLARYA
EEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHEEHHHHCCCCHHHHHHHH
REHGFEVRYIEFMPLDAQNLWDRGKVLLADDMIDQLSREISPLSPLPDPDPRAPATEYVY
HHCCCEEEEEEEEECCHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
QDGGGTVGFIASVSRPFCLNCNRLRITADGKLRYCLFAINEDDVKTLMRSGASDEAIAAL
ECCCCEEEEEECCCCCEEECCCEEEEEECCCEEEEEEEECCHHHHHHHHCCCCHHHHHHH
IRRNVAAKWEGHEINSSKFVAPPRPMYSIGG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]