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The map label for this gene is spsL [H]

Identifier: 116625282

GI number: 116625282

Start: 7805102

End: 7805623

Strand: Direct

Name: spsL [H]

Synonym: Acid_6227

Alternate gene names: 116625282

Gene position: 7805102-7805623 (Clockwise)

Preceding gene: 116625281

Following gene: 116625283

Centisome position: 78.32

GC content: 59.96

Gene sequence:

>522_bases
GTGGTCCCCGAATGTGCCCGGGGAATCGGCAGCCTGATCACCTCTCCGTCATCGGAGCACCTGATCTCGGGCGTCAAAGT
CGAGCCCTACGCGCTCTTTCCGGACGATCGCGGCTACTTCCTTGAAGTTCATCGCATGGGCCGCGGCCTCGCCGCAGCTT
TCCCTCCAGCGACCACCCAGATGTCGGCGGCATTAAACTATCCCGGCACCGTTAAGGCCTTCCATTTTCATCTCCACCAG
CACGATTGCTGGGCACCCGTGTACGGCCTGCTGCAGGTGGCCCTGGTGGATCTGCGCCTCGGTTCGGCGACTTACGGCGC
ACGGAACACTCTCTACGTCGGCGCGCTCCGTCCCTGGCAGATCCTGATTCCGCCAGGCGTGGGGCACGGCTACAAAGTAA
TCGGTAACAAGGAAGCCATGCTGGTCTATCTGACCGACCAGTTTTACAATCCCAAGGACGAGGGCCGTGTTCCGTACAAC
GACCCCGGAATCAATTACGATTGGGAGACGCAGAATAAATAG

Upstream 100 bases:

>100_bases
AATTCGCCGCCGAACCGGAAAGGGCGGTCCGGATCTGATGGGTGCCGAAGCGCTACAGGCCGGTGGACGCCGCCTGGCCT
CACATAACGATCTGCAACTG

Downstream 100 bases:

>100_bases
CTTTTCCGGCTGCCATCCGGCGGCCTTTAGGATCTTTTCTTCATGAAAATTCTGGTAACGGGTGGGGCAGGTTTTATCGG
GTCTGCGTTTGTGCGGATGG

Product: dTDP-4-dehydrorhamnose 3,5-epimerase related

Products: dTDP-4-dehydro-6-deoxy-L-mannose

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MVPECARGIGSLITSPSSEHLISGVKVEPYALFPDDRGYFLEVHRMGRGLAAAFPPATTQMSAALNYPGTVKAFHFHLHQ
HDCWAPVYGLLQVALVDLRLGSATYGARNTLYVGALRPWQILIPPGVGHGYKVIGNKEAMLVYLTDQFYNPKDEGRVPYN
DPGINYDWETQNK

Sequences:

>Translated_173_residues
MVPECARGIGSLITSPSSEHLISGVKVEPYALFPDDRGYFLEVHRMGRGLAAAFPPATTQMSAALNYPGTVKAFHFHLHQ
HDCWAPVYGLLQVALVDLRLGSATYGARNTLYVGALRPWQILIPPGVGHGYKVIGNKEAMLVYLTDQFYNPKDEGRVPYN
DPGINYDWETQNK
>Mature_173_residues
MVPECARGIGSLITSPSSEHLISGVKVEPYALFPDDRGYFLEVHRMGRGLAAAFPPATTQMSAALNYPGTVKAFHFHLHQ
HDCWAPVYGLLQVALVDLRLGSATYGARNTLYVGALRPWQILIPPGVGHGYKVIGNKEAMLVYLTDQFYNPKDEGRVPYN
DPGINYDWETQNK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1898

COG function: function code M; dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To dTDP-4-dehydrorhamnose reductase [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR000888
- InterPro:   IPR014710
- ProDom:   PD001462 [H]

Pfam domain/function: PF00908 dTDP_sugar_isom [H]

EC number: 5.1.3.13

Molecular weight: Translated: 19175; Mature: 19175

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPECARGIGSLITSPSSEHLISGVKVEPYALFPDDRGYFLEVHRMGRGLAAAFPPATTQ
CCCHHHHHHHHHHCCCCHHHHHCCCEECCEEECCCCCCEEEEEEECCCCCEECCCCCCCC
MSAALNYPGTVKAFHFHLHQHDCWAPVYGLLQVALVDLRLGSATYGARNTLYVGALRPWQ
EEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEE
ILIPPGVGHGYKVIGNKEAMLVYLTDQFYNPKDEGRVPYNDPGINYDWETQNK
EEECCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVPECARGIGSLITSPSSEHLISGVKVEPYALFPDDRGYFLEVHRMGRGLAAAFPPATTQ
CCCHHHHHHHHHHCCCCHHHHHCCCEECCEEECCCCCCEEEEEEECCCCCEECCCCCCCC
MSAALNYPGTVKAFHFHLHQHDCWAPVYGLLQVALVDLRLGSATYGARNTLYVGALRPWQ
EEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEE
ILIPPGVGHGYKVIGNKEAMLVYLTDQFYNPKDEGRVPYNDPGINYDWETQNK
EEECCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.025 {dTDP-4-dehydro-6-deoxy-D-glucose}} [C]

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose

General reaction: Epimerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]