Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 116625283

GI number: 116625283

Start: 7805666

End: 7806685

Strand: Direct

Name: rfbB [H]

Synonym: Acid_6228

Alternate gene names: 116625283

Gene position: 7805666-7806685 (Clockwise)

Preceding gene: 116625282

Following gene: 116625284

Centisome position: 78.33

GC content: 60.78

Gene sequence:

>1020_bases
ATGAAAATTCTGGTAACGGGTGGGGCAGGTTTTATCGGGTCTGCGTTTGTGCGGATGGCGATCGCGGAAACGGACCTTCA
CGTAGTGAATCTCGACAAATTGACCTACGCCGGGAACCTCGAGAACCTGGCGCCGGTGGATGGCAGCGATCGCTATCGCT
TCGTCCATGGCGATATCTGCGATGCCGCGCTGGTCGAAGCCACGCTCGCGGAAGAGCGGCCCGATGCCATCGTTCACTTT
GCCGCCGAGTCGCACGTTGACCGCAGCATCCTCTCGCCCGAGCCGGTCGTGCGCACCAATTACAACGGAACCTTCACGCT
TCTCGAAGCCGCCCGCCGTCAGAAAATCGCCCGCTTCGTTCACGTCTCCACCGACGAAGTCTATGGCAGCCTCGAAGCGC
CCGCCGAAGCCGATGAGGCGTACGTGCTCAATCCGAGCAGCCCCTATTCGGCATCGAAGGCCGCCAGCGACCTGCTCGCG
CGCTCTTACTTCGTCACTTACAAGCTGCCCGTCCTGATCACTCGCGCGTCCAATAACTACGGGCCCTATCAGTTTCCCGA
GAAGTTGATCCCGCTGATGATCGCCAATGCCCTGGACGACCAGCCGCTGCCCGTCTATGGCGATGGCCAGCAGGTTCGCG
ACTGGCTCTACGTGTACGATCACTGCCGCGGAATTATGGCCGTCCTCAACCAGGGCCGCGAAGGCGAGATCTACAACATC
GGCGGCAATCGCTCGCTGCCTAATCTCGACGTGATCCATCAGGTGCTCGCGATTACCGGAAAGAGCGAAAGCCTCATCGT
CTACGTGAAAGATCGGCCCGGCCACGATCGCCGCTACGCCCTTTCGAGCGAGAAGCTCATGCACGAAACCGGTTGGCAGC
CTCTGATGGATTTCGAAACCGGCCTCGCGCGCACCATCGAGTGGTACCGCGGGAATGCGGCCTGGGTGGCTCGCGTGCGC
AGCGGCGAATATCGCAATTACTACGAACGCAACTACGGCAACCGCACCGCCGTTTCCTAG

Upstream 100 bases:

>100_bases
CCGTGTTCCGTACAACGACCCCGGAATCAATTACGATTGGGAGACGCAGAATAAATAGCTTTTCCGGCTGCCATCCGGCG
GCCTTTAGGATCTTTTCTTC

Downstream 100 bases:

>100_bases
CCGCTGGATTGAATGCCCACTCCTCCAATCCTTCTCGCGTCCCGGCCCGCTCCAATCCGAGCCGCGACCGTTAGGGAGCG
GTCGCTAATTTTACTGTACA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKILVTGGAGFIGSAFVRMAIAETDLHVVNLDKLTYAGNLENLAPVDGSDRYRFVHGDICDAALVEATLAEERPDAIVHF
AAESHVDRSILSPEPVVRTNYNGTFTLLEAARRQKIARFVHVSTDEVYGSLEAPAEADEAYVLNPSSPYSASKAASDLLA
RSYFVTYKLPVLITRASNNYGPYQFPEKLIPLMIANALDDQPLPVYGDGQQVRDWLYVYDHCRGIMAVLNQGREGEIYNI
GGNRSLPNLDVIHQVLAITGKSESLIVYVKDRPGHDRRYALSSEKLMHETGWQPLMDFETGLARTIEWYRGNAAWVARVR
SGEYRNYYERNYGNRTAVS

Sequences:

>Translated_339_residues
MKILVTGGAGFIGSAFVRMAIAETDLHVVNLDKLTYAGNLENLAPVDGSDRYRFVHGDICDAALVEATLAEERPDAIVHF
AAESHVDRSILSPEPVVRTNYNGTFTLLEAARRQKIARFVHVSTDEVYGSLEAPAEADEAYVLNPSSPYSASKAASDLLA
RSYFVTYKLPVLITRASNNYGPYQFPEKLIPLMIANALDDQPLPVYGDGQQVRDWLYVYDHCRGIMAVLNQGREGEIYNI
GGNRSLPNLDVIHQVLAITGKSESLIVYVKDRPGHDRRYALSSEKLMHETGWQPLMDFETGLARTIEWYRGNAAWVARVR
SGEYRNYYERNYGNRTAVS
>Mature_339_residues
MKILVTGGAGFIGSAFVRMAIAETDLHVVNLDKLTYAGNLENLAPVDGSDRYRFVHGDICDAALVEATLAEERPDAIVHF
AAESHVDRSILSPEPVVRTNYNGTFTLLEAARRQKIARFVHVSTDEVYGSLEAPAEADEAYVLNPSSPYSASKAASDLLA
RSYFVTYKLPVLITRASNNYGPYQFPEKLIPLMIANALDDQPLPVYGDGQQVRDWLYVYDHCRGIMAVLNQGREGEIYNI
GGNRSLPNLDVIHQVLAITGKSESLIVYVKDRPGHDRRYALSSEKLMHETGWQPLMDFETGLARTIEWYRGNAAWVARVR
SGEYRNYYERNYGNRTAVS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=39.8119122257053, Blast_Score=256, Evalue=1e-68,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=27.0348837209302, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=27.3529411764706, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=27.3529411764706, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=27.3529411764706, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=52.2222222222222, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=51.5942028985507, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=26.2048192771084, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788366, Length=240, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=36.8580060422961, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=34.7003154574132, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=320, Percent_Identity=26.25, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=346, Percent_Identity=26.5895953757225, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=241, Percent_Identity=25.3112033195021, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=346, Percent_Identity=25.4335260115607, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=28.3076923076923, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37871; Mature: 37871

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAFVRMAIAETDLHVVNLDKLTYAGNLENLAPVDGSDRYRFVHGDIC
CEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCCCEEEEECCHH
DAALVEATLAEERPDAIVHFAAESHVDRSILSPEPVVRTNYNGTFTLLEAARRQKIARFV
HHHHHHHHHHCCCCCEEEEEEHHHCCCHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHH
HVSTDEVYGSLEAPAEADEAYVLNPSSPYSASKAASDLLARSYFVTYKLPVLITRASNNY
CCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEEEEEEEEECCCCC
GPYQFPEKLIPLMIANALDDQPLPVYGDGQQVRDWLYVYDHCRGIMAVLNQGREGEIYNI
CCCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
GGNRSLPNLDVIHQVLAITGKSESLIVYVKDRPGHDRRYALSSEKLMHETGWQPLMDFET
CCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCHHHHHHHCCCCCHHHHHH
GLARTIEWYRGNAAWVARVRSGEYRNYYERNYGNRTAVS
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAFVRMAIAETDLHVVNLDKLTYAGNLENLAPVDGSDRYRFVHGDIC
CEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCCCEEEEECCHH
DAALVEATLAEERPDAIVHFAAESHVDRSILSPEPVVRTNYNGTFTLLEAARRQKIARFV
HHHHHHHHHHCCCCCEEEEEEHHHCCCHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHH
HVSTDEVYGSLEAPAEADEAYVLNPSSPYSASKAASDLLARSYFVTYKLPVLITRASNNY
CCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEEEEEEEEECCCCC
GPYQFPEKLIPLMIANALDDQPLPVYGDGQQVRDWLYVYDHCRGIMAVLNQGREGEIYNI
CCCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
GGNRSLPNLDVIHQVLAITGKSESLIVYVKDRPGHDRRYALSSEKLMHETGWQPLMDFET
CCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCHHHHHHHCCCCCHHHHHH
GLARTIEWYRGNAAWVARVRSGEYRNYYERNYGNRTAVS
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]