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The map label for this gene is spsI [H]

Identifier: 116625281

GI number: 116625281

Start: 7804266

End: 7805039

Strand: Direct

Name: spsI [H]

Synonym: Acid_6226

Alternate gene names: 116625281

Gene position: 7804266-7805039 (Clockwise)

Preceding gene: 116625275

Following gene: 116625282

Centisome position: 78.31

GC content: 60.34

Gene sequence:

>774_bases
ATGAAGGGTGTCGTTTTAGCCGGGGGTACCGGCAGCCGTCTGTTTCCGCTGACCAAGATCACGAATAAACATCTGCTGCC
GATCTACGACCGGCCGATGATTTATTACCCGATCCAGACGCTCGTCGATGCGGGCATCCAGGAGATCCTGATCGTCACCG
GCGGTCGGAATTCCGGCGATTTTCTGCGCCTGCTGGCCAATGGAAAGCAGTTCGGCCTCAAGCACATCAACTATACGTAC
CAGGAAGGCGAGGGCGGCATCGCCGACGCGCTGGCCCTGGCCGAGCATTTCGCCGACGGCCACCAGATCTGCGTCGTCCT
CGGCGACAATATCATCGAAGGCAACATTAAGGCCGCCGCCGATCGTTTCCGCGCTCAGCCTGCCGGCGCCCACATTCTGC
TCAAGGAAGTTCAGGACGCGGAGCGCTTCGGCGTGGCTGAAGTCGCCGGCGACCGCATCGTGGGTATCGAAGAGAAGCCC
AACAACCCCAAATCGAATTACGCCGTGACCGGCATCTATATGTACGATGCCAGCGTCTTCCAGAAGATCAAAACCCTGGT
TCCCTCGGGCCGCGGCGAGCTCGAAATCACCGACGTCAATAACGCCTACATCCGGGAAGGCAACATGAGCTTCTCTTACC
TGGATGGCTGGTGGACCGACGCCGGAACCTTCGATTCCCTGCTGCGCGCCACCAATCTTGTAGCCCAGTCCGCCGCCAAG
ACGGTGGCCGTCCCGGAATTCGCCGCCGAACCGGAAAGGGCGGTCCGGATCTGA

Upstream 100 bases:

>100_bases
CAACATCAGTATCAGTCCTCTCATGTCTCGATTTCCTCCCGGAGTTTTCATTATGCTCGCGTGCCAGGCCGATTGCGTCG
GTACGGTATAATTAACGCCT

Downstream 100 bases:

>100_bases
TGGGTGCCGAAGCGCTACAGGCCGGTGGACGCCGCCTGGCCTCACATAACGATCTGCAACTGGTGGTCCCCGAATGTGCC
CGGGGAATCGGCAGCCTGAT

Product: nucleotidyl transferase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGGRNSGDFLRLLANGKQFGLKHINYTY
QEGEGGIADALALAEHFADGHQICVVLGDNIIEGNIKAAADRFRAQPAGAHILLKEVQDAERFGVAEVAGDRIVGIEEKP
NNPKSNYAVTGIYMYDASVFQKIKTLVPSGRGELEITDVNNAYIREGNMSFSYLDGWWTDAGTFDSLLRATNLVAQSAAK
TVAVPEFAAEPERAVRI

Sequences:

>Translated_257_residues
MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGGRNSGDFLRLLANGKQFGLKHINYTY
QEGEGGIADALALAEHFADGHQICVVLGDNIIEGNIKAAADRFRAQPAGAHILLKEVQDAERFGVAEVAGDRIVGIEEKP
NNPKSNYAVTGIYMYDASVFQKIKTLVPSGRGELEITDVNNAYIREGNMSFSYLDGWWTDAGTFDSLLRATNLVAQSAAK
TVAVPEFAAEPERAVRI
>Mature_257_residues
MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGGRNSGDFLRLLANGKQFGLKHINYTY
QEGEGGIADALALAEHFADGHQICVVLGDNIIEGNIKAAADRFRAQPAGAHILLKEVQDAERFGVAEVAGDRIVGIEEKP
NNPKSNYAVTGIYMYDASVFQKIKTLVPSGRGELEITDVNNAYIREGNMSFSYLDGWWTDAGTFDSLLRATNLVAQSAAK
TVAVPEFAAEPERAVRI

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=241, Percent_Identity=26.5560165975104, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI11761619, Length=241, Percent_Identity=26.5560165975104, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1790224, Length=238, Percent_Identity=46.6386554621849, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1788351, Length=256, Percent_Identity=43.75, Blast_Score=206, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=23.7903225806452, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=252, Percent_Identity=29.3650793650794, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355443, Length=241, Percent_Identity=25.7261410788382, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644084, Length=241, Percent_Identity=25.7261410788382, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 27954; Mature: 27954

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGGRNSGD
CCCEEEECCCCCCEEEEHECCCCCEEEEECCCEEEECHHHHHHCCCEEEEEEECCCCCCH
FLRLLANGKQFGLKHINYTYQEGEGGIADALALAEHFADGHQICVVLGDNIIEGNIKAAA
HHHEECCCHHHCCEECEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHH
DRFRAQPAGAHILLKEVQDAERFGVAEVAGDRIVGIEEKPNNPKSNYAVTGIYMYDASVF
HHHCCCCCCCEEHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECHHHH
QKIKTLVPSGRGELEITDVNNAYIREGNMSFSYLDGWWTDAGTFDSLLRATNLVAQSAAK
HHHHHHCCCCCCCEEEEECCCEEEECCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHC
TVAVPEFAAEPERAVRI
EEECCHHHCCCHHHCCC
>Mature Secondary Structure
MKGVVLAGGTGSRLFPLTKITNKHLLPIYDRPMIYYPIQTLVDAGIQEILIVTGGRNSGD
CCCEEEECCCCCCEEEEHECCCCCEEEEECCCEEEECHHHHHHCCCEEEEEEECCCCCCH
FLRLLANGKQFGLKHINYTYQEGEGGIADALALAEHFADGHQICVVLGDNIIEGNIKAAA
HHHEECCCHHHCCEECEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHH
DRFRAQPAGAHILLKEVQDAERFGVAEVAGDRIVGIEEKPNNPKSNYAVTGIYMYDASVF
HHHCCCCCCCEEHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECHHHH
QKIKTLVPSGRGELEITDVNNAYIREGNMSFSYLDGWWTDAGTFDSLLRATNLVAQSAAK
HHHHHHCCCCCCCEEEEECCCEEEECCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHC
TVAVPEFAAEPERAVRI
EEECCHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]