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The map label for this gene is purF [H]

Identifier: 116625015

GI number: 116625015

Start: 7453862

End: 7455280

Strand: Direct

Name: purF [H]

Synonym: Acid_5945

Alternate gene names: 116625015

Gene position: 7453862-7455280 (Clockwise)

Preceding gene: 116625014

Following gene: 116625017

Centisome position: 74.8

GC content: 62.72

Gene sequence:

>1419_bases
ATGTTCGATAAATTTCACGATGAATGCGGCGTGGTCGCCATTTACGGCCACCCCGAGGCTTCTAAACTTGCGTATCTCGG
GCTCTACTCGCTCCAGCATCGCGGGCAGGAGAGCGCCGGCATCTGCACGTCGGATGGCATCGAGGTCCACACCCACAAGA
CGATGGGGCACGTGGCGGATATCTTCACCAGCGATGTGCTGGCAACGCTGCCGGGCCCGCTCGCGATCGGACACACACGC
TATTCCACCGCCGGCGACACGGTGCTGCTGAACGCCCAGCCCTTCTCGGTGGATTGCAACAAGGGCAAGGTCGCGATCGC
GCACAACGGCAACATCACCAACGCGACGGAACTCCGCAAAGACCTGGAGCGGCGCGGCGCGATCTTCCAGGCGTCGAGCG
ACACCGAAGTCGTGCTGCACCTGATGGCGCACTCTTCGGAACGCACGCTGGCCGGGGCGCTGCGCGACGCGCTGCTGCAA
CTGGAGGGAGCATTCTCCCTGGTATTTCTGGCGGAGGACCGCGTCATTGTGGCGCGCGATCCGCACGGCTTCCGTCCGCT
CGCCATGGGCGAGATGGAGGTGTCGGGCGGACGCAAGTGCACCGTCTTCGCCTCCGAGACCTGCGCCTTCGACCTGATCG
GAGCGGTCTATTTGCACGATGTAGAGCCAGGTGAAATGGTAATCGTGGGACCCGAAGGGGTGACGCGTGAACGCTACGCG
CCGGCGCAGGCGCGGTCGCAATGCGTTTTCGAACACGTTTATTTCGCACGCCCCGATTCCATCGTCTTCGGACGCGCAGT
GGAAGAATCGCGGGAGAATTTGGGGCGCCTGCTGGCGCGCGAATGTCCAGCGGATGCAGACCTGGTGGTTCCCGTGCCGG
ATTCCGGCGTGGCCGCCGCGATCGGATATGCCGATGAATCGGGACTTCCCTTCCGCCAGGCGCTGATCCGCAATCATTAT
GTGGGCCGCACGTTCATCGAGCCCTCGCAGGCGATCCGCGATTTCGGAGTCAAGTTGAAGCTCAACCCGGTGCGGCACCT
GCTGCAGGGTAAGCGGGTCGTGCTGGTGGATGATTCGATCGTGCGCGGTACTACCAGCCGTAAGATCGTCCGCATGGTTC
GCCAGGCCGGCGCGCGCGAAGTGCATCTGCGCATCTCCTGTCCGCCCACGATTTCGCCCTGCTTCTATGGCGTCGATACG
CCGACGCGCAGCGAATTGATCGCGTCCAATCACAGCGTGGAAGAGATCCGCCGCTTCGTGGAAGCCGACTCGGTCGGCTA
TCTTTCGATCGGCTCCCTGCGTAAAGCGGTCGGCGATGATGAGAAGCACGAATACTGCTACGCGTGCTACACAGGCGACT
ACCCGACCGAGCTCGTGAACATCGAAGAGCTCATTACCGCCCGCCAGAAGCGCAAGTAG

Upstream 100 bases:

>100_bases
ACAATTGAGTCAGGAATGGAAATCCGGCAGAGGGGCAATACGCTGGGTTCCTGGGACATCGCGGCACTCAGGGCCGCTTA
CGAGGGGGCACTCGAGGCGC

Downstream 100 bases:

>100_bases
GACGGGCCTCCTGGCCGGCCCACTAGTTACGAGCTATAGACCGCGACCTTCGTAACCGTGCGAAACCCCATCCGCTCGTA
CATACGGAGTCCCGCGGGCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFDKFHDECGVVAIYGHPEASKLAYLGLYSLQHRGQESAGICTSDGIEVHTHKTMGHVADIFTSDVLATLPGPLAIGHTR
YSTAGDTVLLNAQPFSVDCNKGKVAIAHNGNITNATELRKDLERRGAIFQASSDTEVVLHLMAHSSERTLAGALRDALLQ
LEGAFSLVFLAEDRVIVARDPHGFRPLAMGEMEVSGGRKCTVFASETCAFDLIGAVYLHDVEPGEMVIVGPEGVTRERYA
PAQARSQCVFEHVYFARPDSIVFGRAVEESRENLGRLLARECPADADLVVPVPDSGVAAAIGYADESGLPFRQALIRNHY
VGRTFIEPSQAIRDFGVKLKLNPVRHLLQGKRVVLVDDSIVRGTTSRKIVRMVRQAGAREVHLRISCPPTISPCFYGVDT
PTRSELIASNHSVEEIRRFVEADSVGYLSIGSLRKAVGDDEKHEYCYACYTGDYPTELVNIEELITARQKRK

Sequences:

>Translated_472_residues
MFDKFHDECGVVAIYGHPEASKLAYLGLYSLQHRGQESAGICTSDGIEVHTHKTMGHVADIFTSDVLATLPGPLAIGHTR
YSTAGDTVLLNAQPFSVDCNKGKVAIAHNGNITNATELRKDLERRGAIFQASSDTEVVLHLMAHSSERTLAGALRDALLQ
LEGAFSLVFLAEDRVIVARDPHGFRPLAMGEMEVSGGRKCTVFASETCAFDLIGAVYLHDVEPGEMVIVGPEGVTRERYA
PAQARSQCVFEHVYFARPDSIVFGRAVEESRENLGRLLARECPADADLVVPVPDSGVAAAIGYADESGLPFRQALIRNHY
VGRTFIEPSQAIRDFGVKLKLNPVRHLLQGKRVVLVDDSIVRGTTSRKIVRMVRQAGAREVHLRISCPPTISPCFYGVDT
PTRSELIASNHSVEEIRRFVEADSVGYLSIGSLRKAVGDDEKHEYCYACYTGDYPTELVNIEELITARQKRK
>Mature_472_residues
MFDKFHDECGVVAIYGHPEASKLAYLGLYSLQHRGQESAGICTSDGIEVHTHKTMGHVADIFTSDVLATLPGPLAIGHTR
YSTAGDTVLLNAQPFSVDCNKGKVAIAHNGNITNATELRKDLERRGAIFQASSDTEVVLHLMAHSSERTLAGALRDALLQ
LEGAFSLVFLAEDRVIVARDPHGFRPLAMGEMEVSGGRKCTVFASETCAFDLIGAVYLHDVEPGEMVIVGPEGVTRERYA
PAQARSQCVFEHVYFARPDSIVFGRAVEESRENLGRLLARECPADADLVVPVPDSGVAAAIGYADESGLPFRQALIRNHY
VGRTFIEPSQAIRDFGVKLKLNPVRHLLQGKRVVLVDDSIVRGTTSRKIVRMVRQAGAREVHLRISCPPTISPCFYGVDT
PTRSELIASNHSVEEIRRFVEADSVGYLSIGSLRKAVGDDEKHEYCYACYTGDYPTELVNIEELITARQKRK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=39.4891944990177, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI205277386, Length=165, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI4826742, Length=187, Percent_Identity=32.0855614973262, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788651, Length=473, Percent_Identity=38.6892177589852, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790167, Length=191, Percent_Identity=31.9371727748691, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=474, Percent_Identity=39.662447257384, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17532897, Length=188, Percent_Identity=29.2553191489362, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=188, Percent_Identity=29.2553191489362, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=492, Percent_Identity=34.7560975609756, Blast_Score=256, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=42.2131147540984, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=43.1034482758621, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=41.5478615071283, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI21357745, Length=186, Percent_Identity=30.1075268817204, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51747; Mature: 51747

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKFHDECGVVAIYGHPEASKLAYLGLYSLQHRGQESAGICTSDGIEVHTHKTMGHVAD
CCCCCCCCCCEEEEECCCCHHHEEEHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHH
IFTSDVLATLPGPLAIGHTRYSTAGDTVLLNAQPFSVDCNKGKVAIAHNGNITNATELRK
HHHHHHHHHCCCCEEECCCEECCCCCEEEEECCCCEEECCCCEEEEEECCCCCCHHHHHH
DLERRGAIFQASSDTEVVLHLMAHSSERTLAGALRDALLQLEGAFSLVFLAEDRVIVARD
HHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
PHGFRPLAMGEMEVSGGRKCTVFASETCAFDLIGAVYLHDVEPGEMVIVGPEGVTRERYA
CCCCCCEEECEEEECCCCEEEEEECCCHHHHHHCEEEEEECCCCCEEEECCCCCCCCCCC
PAQARSQCVFEHVYFARPDSIVFGRAVEESRENLGRLLARECPADADLVVPVPDSGVAAA
CHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEE
IGYADESGLPFRQALIRNHYVGRTFIEPSQAIRDFGVKLKLNPVRHLLQGKRVVLVDDSI
EECCCCCCCCHHHHHHHCCCCCCEECCHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCH
VRGTTSRKIVRMVRQAGAREVHLRISCPPTISPCFYGVDTPTRSELIASNHSVEEIRRFV
HCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
EADSVGYLSIGSLRKAVGDDEKHEYCYACYTGDYPTELVNIEELITARQKRK
HCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MFDKFHDECGVVAIYGHPEASKLAYLGLYSLQHRGQESAGICTSDGIEVHTHKTMGHVAD
CCCCCCCCCCEEEEECCCCHHHEEEHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHH
IFTSDVLATLPGPLAIGHTRYSTAGDTVLLNAQPFSVDCNKGKVAIAHNGNITNATELRK
HHHHHHHHHCCCCEEECCCEECCCCCEEEEECCCCEEECCCCEEEEEECCCCCCHHHHHH
DLERRGAIFQASSDTEVVLHLMAHSSERTLAGALRDALLQLEGAFSLVFLAEDRVIVARD
HHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
PHGFRPLAMGEMEVSGGRKCTVFASETCAFDLIGAVYLHDVEPGEMVIVGPEGVTRERYA
CCCCCCEEECEEEECCCCEEEEEECCCHHHHHHCEEEEEECCCCCEEEECCCCCCCCCCC
PAQARSQCVFEHVYFARPDSIVFGRAVEESRENLGRLLARECPADADLVVPVPDSGVAAA
CHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEE
IGYADESGLPFRQALIRNHYVGRTFIEPSQAIRDFGVKLKLNPVRHLLQGKRVVLVDDSI
EECCCCCCCCHHHHHHHCCCCCCEECCHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCH
VRGTTSRKIVRMVRQAGAREVHLRISCPPTISPCFYGVDTPTRSELIASNHSVEEIRRFV
HCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
EADSVGYLSIGSLRKAVGDDEKHEYCYACYTGDYPTELVNIEELITARQKRK
HCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]