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The map label for this gene is gnuT [H]

Identifier: 116624619

GI number: 116624619

Start: 6946397

End: 6947740

Strand: Reverse

Name: gnuT [H]

Synonym: Acid_5543

Alternate gene names: 116624619

Gene position: 6947740-6946397 (Counterclockwise)

Preceding gene: 116624620

Following gene: 116624618

Centisome position: 69.72

GC content: 64.73

Gene sequence:

>1344_bases
ATGCCCGCCAATGGGACTTACCTAATCTGCATTACCGTAGCGACCCTGGCCTGCCTTCTTCTCCTCATCCTGGTCGGAAA
ACTGCACGCCTTTCTGGCTCTCCTGATTTCGTCGATGTTCATGGGCCTCGCCGCCGGAATGGCCCCGGAAAAGGTTCTCA
AGTCCATCCAGACCGGCTTCGGCGATGCTCTCGGATTCATCGCGGTGGTGATCGGCCTCGGCGCCATGATCGGCCGTTTC
CTCGAGTACTCCGGCGGCGGCCGCGCGCTCGCCGACTGGCTGCTCGTCAAGTTCGGCAAGAACCACGCCGCATGGGCCGT
GCTCATTGCCTCTTTCCTCGTCGGACTCCCGATCTTCTTCGAGGTCGGATTCATCATCCTCATCCCCATCGTCTGGAGCC
TCACCCGCGAGACCAAGCGCTCCCTGCTTTTCTACGGGCTGCCGATGGCCGCCGCGCTCACCATGACGCATTCCCTGGTG
CCGCCCCACCCCGCTCCCGCGGCCGCCGCGCAGTTGATGGGGGGCGATCTCGCCCACACCATCATCTACGGCGTGCTGAT
GTCGATCCCGATGACCCTGATTGCCGGCATCGTGTATGCCACCTGGGTGGCGCGCCGCATCTATATCCCCGTCCCCGAAA
TCGCAGCCTCGATGACCCTAAAATCCGCCGAGTCACCCGGCGCGCCGCCGCCCGTGGCCGTCGTCGTCCTGCTCCTGTTG
CTCCCGGTCACGCTCACCTTCGGCGCGACTATGGCCAGCTTGCGCAATATTCCCTTCCGCGGAGTCGCAGTCTTCCTCGG
GCATCCGTACACCGCGCTCACCGTCACCACCCTGATCGCCCTCTACTGCTTCGGCCTGCGCCGCGGATTGTCGCGCGACC
AGGTGCTCAAAATGGCCACGGACTCGCTGCTCCCCGTCGGCGGACTGATGTGCATCATCGGCGGCGGCGGCGCGCTGAAA
CAGGTGATCGTGGATTCCGGCGTTGGAACTTACGCCGGTAAGCTGCTGATGACCAGCGCGATCTCGCCGCTGATCGTGGT
CTGGCTGATCGCCGCCTGCATGCGCCTCGCGCAGGGCTCCGCGACTGTCGCCATCATCACGGCCGCCGGCATCGCCGCGC
CGCTGGTCAAGGGACTGCCCGGCTACTCGCCCGACGAGTTGATCCTCGCCCTCTGCTGCGGCGGCTCGGCCTTTTCCCAG
GTCAGCGATTCGGGCTTCTGGATGGTCACCAATTACTTCGGCACCACCGTCGCCCAGACGCTGAAGACCTGGACCACCAT
GAAGATCATCGCCTCCCTGCTGGGGCTCGCCATCATGCTCACCGCGCATGCGCTGCTACGATAG

Upstream 100 bases:

>100_bases
CATGCAAGGCACCGCCGCTACCGTCGGCCTGGACGTCGGCATCAATGTCTTGCGCGAATTCTGGCCTCGAGGCCGCTGAG
CAGTGTAGAATGGCGGCTTC

Downstream 100 bases:

>100_bases
TGAGTGCATGAGCCTTTACGACTTGGAGCCGGGTCCGGATTGCCCTGAGCTCGTCCGCATGATCGTCGAGATCCCGAAGA
ATTCCAGCAACAAATACGAA

Product: gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPANGTYLICITVATLACLLLLILVGKLHAFLALLISSMFMGLAAGMAPEKVLKSIQTGFGDALGFIAVVIGLGAMIGRF
LEYSGGGRALADWLLVKFGKNHAAWAVLIASFLVGLPIFFEVGFIILIPIVWSLTRETKRSLLFYGLPMAAALTMTHSLV
PPHPAPAAAAQLMGGDLAHTIIYGVLMSIPMTLIAGIVYATWVARRIYIPVPEIAASMTLKSAESPGAPPPVAVVVLLLL
LPVTLTFGATMASLRNIPFRGVAVFLGHPYTALTVTTLIALYCFGLRRGLSRDQVLKMATDSLLPVGGLMCIIGGGGALK
QVIVDSGVGTYAGKLLMTSAISPLIVVWLIAACMRLAQGSATVAIITAAGIAAPLVKGLPGYSPDELILALCCGGSAFSQ
VSDSGFWMVTNYFGTTVAQTLKTWTTMKIIASLLGLAIMLTAHALLR

Sequences:

>Translated_447_residues
MPANGTYLICITVATLACLLLLILVGKLHAFLALLISSMFMGLAAGMAPEKVLKSIQTGFGDALGFIAVVIGLGAMIGRF
LEYSGGGRALADWLLVKFGKNHAAWAVLIASFLVGLPIFFEVGFIILIPIVWSLTRETKRSLLFYGLPMAAALTMTHSLV
PPHPAPAAAAQLMGGDLAHTIIYGVLMSIPMTLIAGIVYATWVARRIYIPVPEIAASMTLKSAESPGAPPPVAVVVLLLL
LPVTLTFGATMASLRNIPFRGVAVFLGHPYTALTVTTLIALYCFGLRRGLSRDQVLKMATDSLLPVGGLMCIIGGGGALK
QVIVDSGVGTYAGKLLMTSAISPLIVVWLIAACMRLAQGSATVAIITAAGIAAPLVKGLPGYSPDELILALCCGGSAFSQ
VSDSGFWMVTNYFGTTVAQTLKTWTTMKIIASLLGLAIMLTAHALLR
>Mature_446_residues
PANGTYLICITVATLACLLLLILVGKLHAFLALLISSMFMGLAAGMAPEKVLKSIQTGFGDALGFIAVVIGLGAMIGRFL
EYSGGGRALADWLLVKFGKNHAAWAVLIASFLVGLPIFFEVGFIILIPIVWSLTRETKRSLLFYGLPMAAALTMTHSLVP
PHPAPAAAAQLMGGDLAHTIIYGVLMSIPMTLIAGIVYATWVARRIYIPVPEIAASMTLKSAESPGAPPPVAVVVLLLLL
PVTLTFGATMASLRNIPFRGVAVFLGHPYTALTVTTLIALYCFGLRRGLSRDQVLKMATDSLLPVGGLMCIIGGGGALKQ
VIVDSGVGTYAGKLLMTSAISPLIVVWLIAACMRLAQGSATVAIITAAGIAAPLVKGLPGYSPDELILALCCGGSAFSQV
SDSGFWMVTNYFGTTVAQTLKTWTTMKIIASLLGLAIMLTAHALLR

Specific function: Part Of The Gluconate Utilization System Gnt-I; High- Affinity Intake Of Gluconate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=422, Percent_Identity=41.7061611374408, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790716, Length=439, Percent_Identity=36.2186788154898, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1789097, Length=442, Percent_Identity=36.4253393665158, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI48994939, Length=446, Percent_Identity=34.7533632286996, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI2367370, Length=451, Percent_Identity=36.3636363636364, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1790776, Length=436, Percent_Identity=32.7981651376147, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788707, Length=433, Percent_Identity=31.8706697459584, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46784; Mature: 46653

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANGTYLICITVATLACLLLLILVGKLHAFLALLISSMFMGLAAGMAPEKVLKSIQTGF
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GDALGFIAVVIGLGAMIGRFLEYSGGGRALADWLLVKFGKNHAAWAVLIASFLVGLPIFF
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EVGFIILIPIVWSLTRETKRSLLFYGLPMAAALTMTHSLVPPHPAPAAAAQLMGGDLAHT
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHH
IIYGVLMSIPMTLIAGIVYATWVARRIYIPVPEIAASMTLKSAESPGAPPPVAVVVLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LPVTLTFGATMASLRNIPFRGVAVFLGHPYTALTVTTLIALYCFGLRRGLSRDQVLKMAT
HHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DSLLPVGGLMCIIGGGGALKQVIVDSGVGTYAGKLLMTSAISPLIVVWLIAACMRLAQGS
HCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ATVAIITAAGIAAPLVKGLPGYSPDELILALCCGGSAFSQVSDSGFWMVTNYFGTTVAQT
CEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHCCCCCCEEEHHHHHHHHHHH
LKTWTTMKIIASLLGLAIMLTAHALLR
HHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PANGTYLICITVATLACLLLLILVGKLHAFLALLISSMFMGLAAGMAPEKVLKSIQTGF
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC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HHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]