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The map label for this gene is gltX

Identifier: 116624096

GI number: 116624096

Start: 6325694

End: 6327112

Strand: Direct

Name: gltX

Synonym: Acid_5011

Alternate gene names: 116624096

Gene position: 6325694-6327112 (Clockwise)

Preceding gene: 116624095

Following gene: 116624097

Centisome position: 63.48

GC content: 62.72

Gene sequence:

>1419_bases
ATGCATAGAGTCCGTTTTGCGCCTTCTCCCACGGGATTTCTGCACATTGGGAGTGCGCGCACCTACATCTTCAACTGGCT
GTTCGCCCGCCATCACGGCGGCACCATGATCCTGCGGATCGATGATACCGATGTCGGGCGCAATAGCGACGCGTCGGAGA
CCTCTATTTTCGACGGGCTTGCGTGGCTCGGATTGGATTGGGACGAGCAGTACCGCCAATCGGAGCGGCTGCCGCTGCAT
CGCGCGATGGCGGATGCAATTCTCACGAAGGGAATGGCCTATCGCGATTTCACTCCGGCGCAGGCTGGAGACAGTGAAAA
ATCCGGCGCCCAGGGAACGTGGCTGTTCAACGCCGGGATGCGCGAGCTTTCGCGCGAAGAGAGTGACCGCCGCGCGGCGG
CGGGCGAGCCGTTCGCGCTGCGGTTCCGCGTGCCGCACGATCGCGAGGGGAGCGTACAGTTCACGGACGCGGTCTACGGA
GAGCAGGCCAAGGGCTACGGCGATATTGAGGATTTCGCGCTGCTGCGCAGCGACGGGATGCCGACATACCATCTGGCAAA
TTGCGCCGATGACGCCGACCTCCGCATTTCGCACATCATTCGGGGCCAGGAGCACCTGTCCAATGCGTTCAAGCACAAGT
TGATCTTCGACGCGGCCGGCGCTGAATCGCCGACCTTCGCGCACCTGCCGCTATTGATGGCGCCCGACGGGATGAAGCTC
TCCAAGCGCGTCCACGGTCCGGTGGTCAGCGTCACCACGTACCGCGACGCGGGATTCCTGCCGCAGGCCTTCATCAACTT
TCTGAGTCTGCTGGGCTGGTCGCCGAAGAATGATCGCGAGAAACTATCGCGCGAAGAGATGGTGGAGCTCTTCACGCTGG
AGGGGATCAACCGTGCTAACGCGGTGGTCAACTTCACCGAGGAAGAACCCTTCGACGCGAAGGCGCTATGGCTGAACGCG
GAGCACATCCGCGCTATGCCGGCGGCGGACCTGGCCCGCGAGTTGTTGCCTTTCGTGCCGGTGGATCTCGAAAAAATGCT
CCAGATCACCCCGCTGATTCAGGAGCGCATCAAACTGCTGCGTGACGTGCAGAGCGTCGCGGATTTCTTCTTTGTGAAGC
AACTGCCGCCGTACGACAGCGCGGAGTTGATCCCCAAGAAGGGCGACGCGGCGATGGCAAAGAACGTCCTGATCAAGGCC
TGCGATGTACTGGCGACGGCCGAGTTCACGCACGACGGCCTGGAAGTGGCGCTGCGCGCAGCCAGCGCGGAGTTGGGCAT
CAAAGCCGGACAGATGTTCGAGCCCGTGCGGGTGGCGGTTTGCGGACGCAAGACCGCGCCGCCCCTGTTCGGCACGCTGG
AAGTTCTCGGCCGCGAGGCCTGCCTGGCGCGGATCGGCCAAGCCATCGAAAAACTGTGA

Upstream 100 bases:

>100_bases
CGCCGGTCCCAACATCCTCTGCTTCACCGCTGATCCTCGCCCGTAAGCCAAGCGCGCGTTTGGCGTGGAGTGCGCGGCAG
GTTGTTATACTCGTATTTCC

Downstream 100 bases:

>100_bases
AAGAGATTATGACCACTGCAAACCCAGAGCCGGCAGCCGGCCCTTCGAATTTCATTCGTGACATCATCCTGGATGACCTG
AACACAAATAAGTACCAGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHRVRFAPSPTGFLHIGSARTYIFNWLFARHHGGTMILRIDDTDVGRNSDASETSIFDGLAWLGLDWDEQYRQSERLPLH
RAMADAILTKGMAYRDFTPAQAGDSEKSGAQGTWLFNAGMRELSREESDRRAAAGEPFALRFRVPHDREGSVQFTDAVYG
EQAKGYGDIEDFALLRSDGMPTYHLANCADDADLRISHIIRGQEHLSNAFKHKLIFDAAGAESPTFAHLPLLMAPDGMKL
SKRVHGPVVSVTTYRDAGFLPQAFINFLSLLGWSPKNDREKLSREEMVELFTLEGINRANAVVNFTEEEPFDAKALWLNA
EHIRAMPAADLARELLPFVPVDLEKMLQITPLIQERIKLLRDVQSVADFFFVKQLPPYDSAELIPKKGDAAMAKNVLIKA
CDVLATAEFTHDGLEVALRAASAELGIKAGQMFEPVRVAVCGRKTAPPLFGTLEVLGREACLARIGQAIEKL

Sequences:

>Translated_472_residues
MHRVRFAPSPTGFLHIGSARTYIFNWLFARHHGGTMILRIDDTDVGRNSDASETSIFDGLAWLGLDWDEQYRQSERLPLH
RAMADAILTKGMAYRDFTPAQAGDSEKSGAQGTWLFNAGMRELSREESDRRAAAGEPFALRFRVPHDREGSVQFTDAVYG
EQAKGYGDIEDFALLRSDGMPTYHLANCADDADLRISHIIRGQEHLSNAFKHKLIFDAAGAESPTFAHLPLLMAPDGMKL
SKRVHGPVVSVTTYRDAGFLPQAFINFLSLLGWSPKNDREKLSREEMVELFTLEGINRANAVVNFTEEEPFDAKALWLNA
EHIRAMPAADLARELLPFVPVDLEKMLQITPLIQERIKLLRDVQSVADFFFVKQLPPYDSAELIPKKGDAAMAKNVLIKA
CDVLATAEFTHDGLEVALRAASAELGIKAGQMFEPVRVAVCGRKTAPPLFGTLEVLGREACLARIGQAIEKL
>Mature_472_residues
MHRVRFAPSPTGFLHIGSARTYIFNWLFARHHGGTMILRIDDTDVGRNSDASETSIFDGLAWLGLDWDEQYRQSERLPLH
RAMADAILTKGMAYRDFTPAQAGDSEKSGAQGTWLFNAGMRELSREESDRRAAAGEPFALRFRVPHDREGSVQFTDAVYG
EQAKGYGDIEDFALLRSDGMPTYHLANCADDADLRISHIIRGQEHLSNAFKHKLIFDAAGAESPTFAHLPLLMAPDGMKL
SKRVHGPVVSVTTYRDAGFLPQAFINFLSLLGWSPKNDREKLSREEMVELFTLEGINRANAVVNFTEEEPFDAKALWLNA
EHIRAMPAADLARELLPFVPVDLEKMLQITPLIQERIKLLRDVQSVADFFFVKQLPPYDSAELIPKKGDAAMAKNVLIKA
CDVLATAEFTHDGLEVALRAASAELGIKAGQMFEPVRVAVCGRKTAPPLFGTLEVLGREACLARIGQAIEKL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=31.6430020283976, Blast_Score=200, Evalue=3e-51,
Organism=Escherichia coli, GI1788743, Length=478, Percent_Identity=35.3556485355649, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=32.3770491803279, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=504, Percent_Identity=30.7539682539683, Blast_Score=219, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=31.1111111111111, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SOLUE (Q01WJ8)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826252.1
- ProteinModelPortal:   Q01WJ8
- SMR:   Q01WJ8
- STRING:   Q01WJ8
- GeneID:   4426759
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5011
- NMPDR:   fig|234267.9.peg.4756
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52434; Mature: 52434

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRVRFAPSPTGFLHIGSARTYIFNWLFARHHGGTMILRIDDTDVGRNSDASETSIFDGL
CCCEECCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCCHHHHHHCH
AWLGLDWDEQYRQSERLPLHRAMADAILTKGMAYRDFTPAQAGDSEKSGAQGTWLFNAGM
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
RELSREESDRRAAAGEPFALRFRVPHDREGSVQFTDAVYGEQAKGYGDIEDFALLRSDGM
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHCCCC
PTYHLANCADDADLRISHIIRGQEHLSNAFKHKLIFDAAGAESPTFAHLPLLMAPDGMKL
CCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCEEECCEEECCCCCHH
SKRVHGPVVSVTTYRDAGFLPQAFINFLSLLGWSPKNDREKLSREEMVELFTLEGINRAN
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AVVNFTEEEPFDAKALWLNAEHIRAMPAADLARELLPFVPVDLEKMLQITPLIQERIKLL
EEEEECCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RDVQSVADFFFVKQLPPYDSAELIPKKGDAAMAKNVLIKACDVLATAEFTHDGLEVALRA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ASAELGIKAGQMFEPVRVAVCGRKTAPPLFGTLEVLGREACLARIGQAIEKL
HHHHHCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRVRFAPSPTGFLHIGSARTYIFNWLFARHHGGTMILRIDDTDVGRNSDASETSIFDGL
CCCEECCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCCHHHHHHCH
AWLGLDWDEQYRQSERLPLHRAMADAILTKGMAYRDFTPAQAGDSEKSGAQGTWLFNAGM
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
RELSREESDRRAAAGEPFALRFRVPHDREGSVQFTDAVYGEQAKGYGDIEDFALLRSDGM
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHCCCC
PTYHLANCADDADLRISHIIRGQEHLSNAFKHKLIFDAAGAESPTFAHLPLLMAPDGMKL
CCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCEEECCEEECCCCCHH
SKRVHGPVVSVTTYRDAGFLPQAFINFLSLLGWSPKNDREKLSREEMVELFTLEGINRAN
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AVVNFTEEEPFDAKALWLNAEHIRAMPAADLARELLPFVPVDLEKMLQITPLIQERIKLL
EEEEECCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RDVQSVADFFFVKQLPPYDSAELIPKKGDAAMAKNVLIKACDVLATAEFTHDGLEVALRA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ASAELGIKAGQMFEPVRVAVCGRKTAPPLFGTLEVLGREACLARIGQAIEKL
HHHHHCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA