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The map label for this gene is pyk [H]

Identifier: 116624087

GI number: 116624087

Start: 6308616

End: 6310028

Strand: Reverse

Name: pyk [H]

Synonym: Acid_5001

Alternate gene names: 116624087

Gene position: 6310028-6308616 (Counterclockwise)

Preceding gene: 116624088

Following gene: 116624085

Centisome position: 63.32

GC content: 62.7

Gene sequence:

>1413_bases
ATGCCAAATACAAAAATTGTAGCCACACTTGGGCCGGCCACAGATGCGCCCGGTGTCTTGCGTCAACTGCTCGCCGCAGG
CGTCGATGTTTTTCGACTGAATGCATCGCACGGAGCACAGGATGCGCATGCCGCGCGGATCGACGCGGTGCGAGCTGCGG
CGCGCGAGGCGCACGTTCACGCCGGGATTCTGCTGGATTTGCAGGGGCCGAAGATCCGGCTCGGGCGCTTTGAAAATGGC
GGAGTGACGCTGGTGACCGACGCCATATTTACGATCACGACAGAGCAGGTGATGGGCACCTCCGAGCGCGCATCGACGGG
CTATTCGCGGTTCGCGAAGGACGTGAAGGCGGGCGATCGCATTCTGCTCGCCGATGGCATCATCGAACTGATCGCGCTCG
AATCGGACGGTGTCAGTGTGCAAACCCGTATCGTGAACGGCGGTCCGATCGGCGACCACAAGGGGATCAATCTTCCGGGG
GTGCAGGTGAGCATTCCCTCTCTGACCGAGAAGGACCTGGCCGATCTGCATTTTGGATTGAACGCCGGAGTCGATATCGT
GGCGCTTTCGTTTGTGCGCACGGCGGACGATGTGCGGCAGTTGCGCGACCGGCTCGGGGGGCGGCCGGTCTCGATTGTCG
CCAAGATCGAAAAGCCGGAGGGCTACGAGAATATCGAGCCGATTCTGGATGTGACGGATGGCGTGATGGTGGCGCGCGGG
GACCTGGGCGTAGAGATTTCGCTGGAGCGTGTGCCGCGAATTCAGAAATCGATCATCCGGCGCGCGCGGCGCAAGGGTCG
GTTCGTGATTACGGCGACGCAGATGCTGGAATCCATGATCGAGAATCCACGGCCCACACGGGCCGAGGTGAGCGATGTCG
CCAATGCGATTTACGATGGCACGGACGCGGTGATGCTGTCGGCGGAGACGTCGGTCGGCAAGTATCCGGTGGAAGCCGTG
CGCTACATGGCGAAGATCGCGGCGGAGAGCGAGGATTCGATTCGCAAGAAGGGATATATCGACCCGCCTCACCAACCGCA
GCCATCGAACGCGGAGATCCTGGCGGATGCGGCGCATCACGCCGCGCGCGATTCGGGGGCGGCGGCGATCGTGGTGTTCA
GTTCGCGCGGATCTAGTGCGCGGCTGGTATCGCGGTACCGTCCGCCGGTAGGGGTGTTCGCGATCACGCCGCACGATACT
ACGGCGCGGCAACTCTCCGTCAGTTACGGCGTGACCCCGCTGCTGGCGCCGGATGTCTCGAGCACGGATGAAATGTTGAG
CCAGATGGACCGCGTGCTCATTGAAGGCGGGTATCTGCAGAAGGGGCAGCTGGTGGTGTTTGTCGCAGGTCAGCCGGTGG
GGCGGCCCGGAACGACGAACCTGATGAAGCTGCATCGCGTGGGTCTGGGATAG

Upstream 100 bases:

>100_bases
CGATTCAGCAAGCAGTCGCACGACTCGATGTCAAACCGGCGAAAGTACCTCCGCGTCTGGCCCCGCATATGGCCCCCCAT
CTGGCAAGATAGTCGGAGGG

Downstream 100 bases:

>100_bases
AGGAACAGACTAGGGGAAACTGACGAGAACGGAGTTGGTGCGTTGGGTATCGACGGTCAGGGTGACGAAGACATCGCCTT
TTGCGGCGAGGGTTCGCGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MPNTKIVATLGPATDAPGVLRQLLAAGVDVFRLNASHGAQDAHAARIDAVRAAAREAHVHAGILLDLQGPKIRLGRFENG
GVTLVTDAIFTITTEQVMGTSERASTGYSRFAKDVKAGDRILLADGIIELIALESDGVSVQTRIVNGGPIGDHKGINLPG
VQVSIPSLTEKDLADLHFGLNAGVDIVALSFVRTADDVRQLRDRLGGRPVSIVAKIEKPEGYENIEPILDVTDGVMVARG
DLGVEISLERVPRIQKSIIRRARRKGRFVITATQMLESMIENPRPTRAEVSDVANAIYDGTDAVMLSAETSVGKYPVEAV
RYMAKIAAESEDSIRKKGYIDPPHQPQPSNAEILADAAHHAARDSGAAAIVVFSSRGSSARLVSRYRPPVGVFAITPHDT
TARQLSVSYGVTPLLAPDVSSTDEMLSQMDRVLIEGGYLQKGQLVVFVAGQPVGRPGTTNLMKLHRVGLG

Sequences:

>Translated_470_residues
MPNTKIVATLGPATDAPGVLRQLLAAGVDVFRLNASHGAQDAHAARIDAVRAAAREAHVHAGILLDLQGPKIRLGRFENG
GVTLVTDAIFTITTEQVMGTSERASTGYSRFAKDVKAGDRILLADGIIELIALESDGVSVQTRIVNGGPIGDHKGINLPG
VQVSIPSLTEKDLADLHFGLNAGVDIVALSFVRTADDVRQLRDRLGGRPVSIVAKIEKPEGYENIEPILDVTDGVMVARG
DLGVEISLERVPRIQKSIIRRARRKGRFVITATQMLESMIENPRPTRAEVSDVANAIYDGTDAVMLSAETSVGKYPVEAV
RYMAKIAAESEDSIRKKGYIDPPHQPQPSNAEILADAAHHAARDSGAAAIVVFSSRGSSARLVSRYRPPVGVFAITPHDT
TARQLSVSYGVTPLLAPDVSSTDEMLSQMDRVLIEGGYLQKGQLVVFVAGQPVGRPGTTNLMKLHRVGLG
>Mature_469_residues
PNTKIVATLGPATDAPGVLRQLLAAGVDVFRLNASHGAQDAHAARIDAVRAAAREAHVHAGILLDLQGPKIRLGRFENGG
VTLVTDAIFTITTEQVMGTSERASTGYSRFAKDVKAGDRILLADGIIELIALESDGVSVQTRIVNGGPIGDHKGINLPGV
QVSIPSLTEKDLADLHFGLNAGVDIVALSFVRTADDVRQLRDRLGGRPVSIVAKIEKPEGYENIEPILDVTDGVMVARGD
LGVEISLERVPRIQKSIIRRARRKGRFVITATQMLESMIENPRPTRAEVSDVANAIYDGTDAVMLSAETSVGKYPVEAVR
YMAKIAAESEDSIRKKGYIDPPHQPQPSNAEILADAAHHAARDSGAAAIVVFSSRGSSARLVSRYRPPVGVFAITPHDTT
ARQLSVSYGVTPLLAPDVSSTDEMLSQMDRVLIEGGYLQKGQLVVFVAGQPVGRPGTTNLMKLHRVGLG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=41.0677618069815, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=41.0677618069815, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=39.1836734693878, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=38.5714285714286, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=38.5714285714286, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=39.1003460207612, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=39.1003460207612, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=36.864406779661, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=36.864406779661, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=36.5740740740741, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=42.9787234042553, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=41.8067226890756, Blast_Score=300, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=478, Percent_Identity=39.9581589958159, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=39.1752577319588, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=39.1752577319588, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=39.1752577319588, Blast_Score=284, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=39.2561983471074, Blast_Score=284, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=39.4628099173554, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=38.8548057259714, Blast_Score=283, Evalue=5e-77,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=38.3673469387755, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=38.3673469387755, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=41.6867469879518, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=30.9917355371901, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24646914, Length=286, Percent_Identity=41.2587412587413, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50148; Mature: 50017

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTKIVATLGPATDAPGVLRQLLAAGVDVFRLNASHGAQDAHAARIDAVRAAAREAHVH
CCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEE
AGILLDLQGPKIRLGRFENGGVTLVTDAIFTITTEQVMGTSERASTGYSRFAKDVKAGDR
EEEEEEECCCEEEEEEECCCCEEEEEEEEEEEEHHHHHCCCHHHHCCHHHHHHHHHCCCE
ILLADGIIELIALESDGVSVQTRIVNGGPIGDHKGINLPGVQVSIPSLTEKDLADLHFGL
EEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCC
NAGVDIVALSFVRTADDVRQLRDRLGGRPVSIVAKIEKPEGYENIEPILDVTDGVMVARG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHCCCCCEEEEEC
DLGVEISLERVPRIQKSIIRRARRKGRFVITATQMLESMIENPRPTRAEVSDVANAIYDG
CCCEEEEHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
TDAVMLSAETSVGKYPVEAVRYMAKIAAESEDSIRKKGYIDPPHQPQPSNAEILADAAHH
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AARDSGAAAIVVFSSRGSSARLVSRYRPPVGVFAITPHDTTARQLSVSYGVTPLLAPDVS
HHCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCHHEEEEECCCCEEECCCCC
STDEMLSQMDRVLIEGGYLQKGQLVVFVAGQPVGRPGTTNLMKLHRVGLG
CHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
PNTKIVATLGPATDAPGVLRQLLAAGVDVFRLNASHGAQDAHAARIDAVRAAAREAHVH
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEE
AGILLDLQGPKIRLGRFENGGVTLVTDAIFTITTEQVMGTSERASTGYSRFAKDVKAGDR
EEEEEEECCCEEEEEEECCCCEEEEEEEEEEEEHHHHHCCCHHHHCCHHHHHHHHHCCCE
ILLADGIIELIALESDGVSVQTRIVNGGPIGDHKGINLPGVQVSIPSLTEKDLADLHFGL
EEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCC
NAGVDIVALSFVRTADDVRQLRDRLGGRPVSIVAKIEKPEGYENIEPILDVTDGVMVARG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHCCCCCEEEEEC
DLGVEISLERVPRIQKSIIRRARRKGRFVITATQMLESMIENPRPTRAEVSDVANAIYDG
CCCEEEEHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
TDAVMLSAETSVGKYPVEAVRYMAKIAAESEDSIRKKGYIDPPHQPQPSNAEILADAAHH
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AARDSGAAAIVVFSSRGSSARLVSRYRPPVGVFAITPHDTTARQLSVSYGVTPLLAPDVS
HHCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCHHEEEEECCCCEEECCCCC
STDEMLSQMDRVLIEGGYLQKGQLVVFVAGQPVGRPGTTNLMKLHRVGLG
CHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]