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The map label for this gene is hybA [C]

Identifier: 116623297

GI number: 116623297

Start: 5328234

End: 5328977

Strand: Direct

Name: hybA [C]

Synonym: Acid_4204

Alternate gene names: 116623297

Gene position: 5328234-5328977 (Clockwise)

Preceding gene: 116623296

Following gene: 116623298

Centisome position: 53.47

GC content: 63.04

Gene sequence:

>744_bases
ATGTCGAAGGCGATACTGTACGACAGCACCCTCTGCGTCGGATGCAAAGCCTGTGAAGGCGCCTGCGCCGAACGCTGGGG
GCTGCCGTACAACGACAAGATTGCCGCGGAAGAGACAATCTCGGCGCACAAGCTCACTACCATCGAAACCCACGGTGAGC
GCTTCAGCCGCCGGTTGTGCATGAACTGCCAGCAACCGGCCTGCGCCAGTGTTTGCCCCGTCGGGGCGCTCCAGAAAACC
GCACTCGGGCCCGTGACCTACGACGCCGATAAGTGTATGGGCTGCCGCTACTGCATGCAGGCCTGCCCCTTCCAGGTGCC
GTCCTACGAATGGAACCAGCGCCTGCCGAAGATGCGCAAGTGCAACATGTGCTACGAACGGCAGAGCCAGGGCAAACCCA
CTGCCTGCGTGGAAGCCTGCCCGGTCGGAGCCACCATCGGCGGCGAACGCGATGCCCTGCTTGCGGAAGCCCGCAAGCGT
ATGGCCGAGAAGCCTGGCGCCTATTACGACAAAGTCTACGGCGTGCAGGAAGCCGGCGGCACCAGCGTCCTTTACCTCTC
CGCGGTGCCCTTCGAACAAATCGGACTCCGCACCAACATCCCCAACGAGAACCTTCCCGAGACCACCTGGCGCGTGCTCG
AACTCGTTCCCGATATCGTCTCCACCGGTTCCGTCCTGCTCGGCGGAATCTGGTGGATCACCAATCGCCGCGCCGAAGTC
GCCAAAAAAGAGGGCCGCCGATGA

Upstream 100 bases:

>100_bases
GAAGTCACCGCCGAGTTCTTCCTGACCGTGTAGGTTTCTCTGTAAAGATTCCCTACACTTTGGCTCAGTTTTCTGTTAAA
AAGGAATTGAAGGTCTTATT

Downstream 100 bases:

>100_bases
CACTCCGTCTTCCCAAACTCACCGTCTGGCGCGCGATCTTCGCCGCCATCATGCTCAGCGGCGCGTATGCCACCTACCTG
AGAATCGTGTATGGCCTGGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSKAILYDSTLCVGCKACEGACAERWGLPYNDKIAAEETISAHKLTTIETHGERFSRRLCMNCQQPACASVCPVGALQKT
ALGPVTYDADKCMGCRYCMQACPFQVPSYEWNQRLPKMRKCNMCYERQSQGKPTACVEACPVGATIGGERDALLAEARKR
MAEKPGAYYDKVYGVQEAGGTSVLYLSAVPFEQIGLRTNIPNENLPETTWRVLELVPDIVSTGSVLLGGIWWITNRRAEV
AKKEGRR

Sequences:

>Translated_247_residues
MSKAILYDSTLCVGCKACEGACAERWGLPYNDKIAAEETISAHKLTTIETHGERFSRRLCMNCQQPACASVCPVGALQKT
ALGPVTYDADKCMGCRYCMQACPFQVPSYEWNQRLPKMRKCNMCYERQSQGKPTACVEACPVGATIGGERDALLAEARKR
MAEKPGAYYDKVYGVQEAGGTSVLYLSAVPFEQIGLRTNIPNENLPETTWRVLELVPDIVSTGSVLLGGIWWITNRRAEV
AKKEGRR
>Mature_246_residues
SKAILYDSTLCVGCKACEGACAERWGLPYNDKIAAEETISAHKLTTIETHGERFSRRLCMNCQQPACASVCPVGALQKTA
LGPVTYDADKCMGCRYCMQACPFQVPSYEWNQRLPKMRKCNMCYERQSQGKPTACVEACPVGATIGGERDALLAEARKRM
AEKPGAYYDKVYGVQEAGGTSVLYLSAVPFEQIGLRTNIPNENLPETTWRVLELVPDIVSTGSVLLGGIWWITNRRAEVA
KKEGRR

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=275, Percent_Identity=36.7272727272727, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787749, Length=200, Percent_Identity=32.5, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790326, Length=199, Percent_Identity=31.6582914572864, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787122, Length=155, Percent_Identity=38.0645161290323, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI2367345, Length=196, Percent_Identity=33.1632653061224, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787872, Length=155, Percent_Identity=37.4193548387097, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI226510944, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787478, Length=113, Percent_Identity=36.283185840708, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788811, Length=149, Percent_Identity=30.2013422818792, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787740, Length=84, Percent_Identity=41.6666666666667, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI87082180, Length=149, Percent_Identity=32.8859060402685, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=149, Percent_Identity=29.5302013422819, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789067, Length=182, Percent_Identity=30.2197802197802, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 27275; Mature: 27143

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAILYDSTLCVGCKACEGACAERWGLPYNDKIAAEETISAHKLTTIETHGERFSRRLC
CCCCEEECCHHHHCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHEEEEHHHHHHHHHHHH
MNCQQPACASVCPVGALQKTALGPVTYDADKCMGCRYCMQACPFQVPSYEWNQRLPKMRK
CCCCCCCHHHHCCCCHHHHHHCCCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHH
CNMCYERQSQGKPTACVEACPVGATIGGERDALLAEARKRMAEKPGAYYDKVYGVQEAGG
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCHHCCCC
TSVLYLSAVPFEQIGLRTNIPNENLPETTWRVLELVPDIVSTGSVLLGGIWWITNRRAEV
CEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEECCHHHH
AKKEGRR
HHHCCCC
>Mature Secondary Structure 
SKAILYDSTLCVGCKACEGACAERWGLPYNDKIAAEETISAHKLTTIETHGERFSRRLC
CCCEEECCHHHHCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHEEEEHHHHHHHHHHHH
MNCQQPACASVCPVGALQKTALGPVTYDADKCMGCRYCMQACPFQVPSYEWNQRLPKMRK
CCCCCCCHHHHCCCCHHHHHHCCCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHH
CNMCYERQSQGKPTACVEACPVGATIGGERDALLAEARKRMAEKPGAYYDKVYGVQEAGG
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCHHCCCC
TSVLYLSAVPFEQIGLRTNIPNENLPETTWRVLELVPDIVSTGSVLLGGIWWITNRRAEV
CEEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEECCHHHH
AKKEGRR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]